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- How a Tourism City Responds to COVID-19: A CEE Perspective (Kraków Case Study)Publication . Kowalczyk-Anioł, Joanna; Grochowicz, Marek; Pawlusiński, RobertAcademic papers on the COVID-19 pandemic and tourism adopt the perspective of strengthening tourism resilience or recognize the ongoing crisis as an opportunity for reflection and the need to redefine tourism development strategies. This article examines these issues using the example of Kraków, a tourism city that, on the eve of the pandemic outbreak, was experiencing symptoms of overtourism. Using a case study approach, the authors illustrate the strategies applied at the city level, as well as the range of measures and actions used to support the tourism industry during the full pandemic year. The Kraków case shows that the pandemic time can be used to reorganize relations between local tourism stakeholders and to create a new formula for a tourism city in the so-called ‘new normal’. In addition, the exploratory case study allows for the formulation of research problems for further work on tourism cities responses to COVID-19. This can focus on a revision of the tourist offer and profile; the role of culture and the accompanying technology-facilitated innovations; the continuation of ‘being-a-tourist-in-your-own-city’, as promoted in the pandemic; tourists’ reactions to introduced changes; and shaping (new) relations between tourism stakeholders in the (post)pandemic city.
- Effects of cadmium and resource quality on freshwater detritus processing chains: a microcosm approach with two insect speciesPublication . Campos, Diana; Alves, Artur; Lemos, Marco F. L.; Correia, António; Soares, Amadeu M. V. M.; Pestana, João L. T.Detritus processing is vital for freshwater ecosystems that depend on the leaf litter from riparian vegetation and is mediated by microorganisms and aquatic invertebrates. Shredder invertebrates transform coarse particulate organic matter into fine particulate organic matter used as food by collector species. Direct and indirect effects of contaminants can impair detritus processing and thus affect the functioning of these ecosystems. Here, we assessed the combined effects of a toxic metal (cadmium) and resource quality (leaf species) on detritus processing and shredder-collector interactions. We considered two types of leaves, alder and eucalyptus that were microbially conditioned under different Cd concentrations in the laboratory. The microbial communities present on leaves were analyzed by Denaturing Gradient Gel Electrophoresis (DGGE), and we also measured microbial respiration rates. Sericostoma vittatum (a caddisfly shredder) and Chironomus riparius (a midge collector) were also exposed to Cd and allowed to consume the corresponding alder or eucalyptus leaves. We evaluated C. riparius growth and leaf mass loss in multispecies microcosms. Cadmium exposure affected leaf conditioning and fungal diversity on both leaf species, as assessed by DGGE. Cadmium exposure also affected the mass loss of alder leaves by reductions in detritivore feeding, and impaired C. riparius growth. Chironomus riparius consumed alder leaf discs in the absence of shredders, but S. vittatum appear to promote C. riparius growth in treatments containing eucalyptus. These results show that indirect effects of contaminants along detritus-processing chains can occur through effects on shredder-collector interactions such as facilitation but they also depend on the nutritional quality of detritus and on sensitivity and feeding plasticity of detritivore species.
- Human milk fat substitutes: Advances and constraints of enzyme‐catalyzed productionPublication . Ferreira-Dias, Suzana; Tecelão, CarlaThe production of triacylglycerols (TAGs) with similar structures to the human milk fat TAGs is only possible using sn-1,3 regioselective lipases as biocatalysts. The advances on the processes currently performed for the production of human milk fat substitutes (HMFS), namely the biocatalysts used, the best operation conditions, reactor types and operation modes, as well as the major drawbacks of these enzymatic systems, are addressed in this article.
- C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in ArabidopsisPublication . Rodriguez, Lesia; Gonzalez-Guzman, Miguel; Diaz, Maira; Rodrigues, Américo; Izquierdo-Garcia, Ana C.; Peirats-Llobet, Marta; Fernandez, Maria A.; Antoni, Regina; Fernandez, Daniel; Marquez, Jose A.; Mulet, Jose M.; Albert, Armando; Rodriguez, Pedro L.Membrane-delimited abscisic acid (ABA) signal transduction plays a critical role in early ABA signaling, but the molecular mechanisms linking core signaling components to the plasma membrane are unclear. We show that transient calcium-dependent interactions of PYR/PYL ABA receptors with membranes are mediated through a 10-member family of C2-domain ABA-related (CAR) proteins in Arabidopsis thaliana. Specifically, we found that PYL4 interacted in an ABA independent manner with CAR1 in both the plasma membrane and nucleus of plant cells. CAR1 belongs to a plant specific gene family encoding CAR1 to CAR10 proteins, and bimolecular fluorescence complementation and coimmunoprecipitation assays showed that PYL4-CAR1 as well as other PYR/PYL-CAR pairs interacted in plant cells. The crystal structure of CAR4 was solved, which revealed that, in addition to a classical calcium-dependent lipid binding C2 domain, a specific CAR signature is likely responsible for the interaction with PYR/PYL receptors and their recruitment to phospholipid vesicles. This interaction is relevant for PYR/PYL function and ABA signaling, since different car triple mutants affected in CAR1, CAR4, CAR5, andCAR9 genes showed reduced sensitivity to ABA in seedling establishment and root growth assays. In summary, we identified PYR/PYL-interacting partners that mediate a transient Ca2+-dependent interaction with phospholipid vesicles, which affects PYR/PYL subcellular localization and positively regulates ABA signaling.
- Topic Prominence of Tourism and Hospitality Scientific Research: The Case of SwitzerlandPublication . Cardoso, Lucília; Soliman, Mohammad; Araújo-Vila, Noelia; Almeida, Giovana Goretti Feijó deTopic prominence is regarded as a recent indicator that reveals the present momentum of a certain topic by considering the citations, views, and CiteScore thresholds. Topic prominence has been examined in prior research within different contexts, however, there is no known study exploring this trend within tourism and hospitality literature. Therefore, this study aims to analyse and map the topic prominence of Tourism, Leisure and Hospitality Management (TLHM) research by focusing on Switzerland as a case study. The collected data included 337 articles published in 46 journals indexed in Scopus under the subject category of TLHM. The findings concluded that topic prominence is an important indicator for measuring scientific research productivity, including peer-reviewed articles. More particularly, it is revealed that the investigation of topic prominence provides an overall clearer picture of Swiss TLHM research. This study contributes to tourism studies by discussing the usage of the topic prominence metric for tourism and hospitality publications. It also presents practical implications for tourism research managers and researchers by providing solid insights into funded research, scholars’ and institutions’ performance, and momentum of topics associated with the Swiss TLHM articles.
- The golden mackerel: first report of a xanthic Mediterranean horse mackerel Trachurus mediterraneus (Steindachner, 1868) from Sines, PortugalPublication . Vasco-Rodrigues, Nuno; Santos, Simão; Espírito-Santo, Cristina; Almada, FredericoIn this study, a single xanthic case of the Mediterranean horse mackerel Trachurus mediterraneus is reported. The individual, with a total lenght (TL) of 306 mm, was captured off Sines (Portugal) in March 2018, by a local fishing boat, using a seine net. This represents the first record of xanthism for this species and for any species of mackerel. The specimen is described here and compared with those of normal pigmentation. Considerations about the paucity of records for this or similar species are presented here, and the possibility of this condition being more frequent than previously thought is discussed.
- Tourist satisfaction: an analysis of tourists visiting historic centres in PortugalPublication . Duque, Ana Sofia; Carvalho, Paulo; Almeida, NunoAchieving tourist satisfaction is one of the main goals of destinations, companies and managers in the field of tourism. To better understand this key concept, we have studied other associated constructs that we call antecedents and consequences, such as tourist motivations, quality of experience and loyalty to the destination. It was decided to achieve the proposed goals by conducting questionnaire surveys with nonresident tourists in Portugal over the three high tourist season months, which are regarded as June, July and August. The sample was collected in five of Portugal’s main historical centres, namely, Porto, Coimbra, Lisbon, Évora and Faro. With this study it was possible to confirm that the level of satisfaction of tourists, who visit historic centers in Portugal, is very high. It was also possible to verify the existing connection between the various constructs developed in the research, through the hypothesis test.
- Dissemination of Antimicrobial-Resistant Clones of Salmonella enterica Among Domestic Animals, Wild Animals, and HumansPublication . Palomo, Gonzalo; Campos, Maria Jorge; Ugarte, María; Porrero, María Concepción; Alonso, Juan Manuel; Borge, Carmen; Vadillo, Santiago; Domínguez, Lucas; Quesada, Alberto; Píriz, SegundoNon-typhoidal salmonellosis is an important zoonotic disease caused by Salmonella enterica. This work focuses on the identification of Salmonella enterica clonal strains which, presenting a wide distribution potential, express resistance determinants that compromise effectiveness of the antimicrobial therapy. The screening was performed on 506 Salmonella enterica isolates from animals and humans, which were characterized by serovar and phage typing, genome macrorestriction and pulsed-field gel electrophoresis, and detection of phenotypic and genotypic traits for antimicrobial resistance. A Salmonella Enteritidis strain with strong quinolone resistance is spread on three host environments carrying one of the four variants found for the GyrA protein: (1) Asp87Tyr, the major polymorphism found in 39 Salmonella isolates from human origin and six from poultry; (2) Ser83Phe, with four isolates from human origin and one from white stork (Ciconia ciconia); and (3) Asp87Asn or (4) Asp87Gly, with two isolates each from human origins. Several Salmonella Typhimurium strains that presented int1 elements and the classically associated pentaresistance (ACSSuT) phenotype were found distributed between two host environments: domestic animals and humans, domestics and wild animals, or wild fauna plus humans. This study points out the importance of monitoring gut microbiota and its antimicrobial resistance from wildlife, in parallel to livestock animals and humans, especially for animal species that are in close contact with people.
- Seasonal Variation in Proximate Composition and Fatty Acid Profile of Grey Triggerfish Balistes capriscus Captured Along the Coast of PortugalPublication . Simões, Tiago; Carvalho, João; Sousa, Ana; Lemos, Marco F. L.; Gil, Maria M.; Pedrosa, Rui; Tecelão, CarlaProximate composition (ash, moisture, total protein, and fat contents) and fatty acid profile of Balistes capriscus (grey triggerfish) were assessed over a 6-months period (April to September, 2011) in animals captured along the coast of Peniche (Portugal). High protein (18.9% to 21.4%) and low lipid (0.5% to 0.8%) contents were determined. The highest lipid level was found in June-captured animals. High proportions of n-3 polyunsaturated fatty acids (n-3 PUFA) were observed (53.87 ± 2.00%) together with substantial quantities of oleic acid (27.58 ± 1.09%) and palmitic acid (16.52 ± 0.93%). Docosahexaenoic acid accounted for 77% of the total PUFA and was 7 times more abundant than eicosapentaenoic acid, and the n-3/n-6 ratio was 7.2. Gender did not influence lipid levels and the fatty acid profile, but seasonal variations were observed for PUFA and monounsaturated fatty acid contents. Overall, the nutritional properties of this underexploited species may be comparable to those of other lean fish species with higher economic value. Practical Application: Balistes capriscus is an abundant species in the Atlantic and underexploited in the Eastern Atlantic coast, with very few studies reported. This study was aimed to add commercial value to the species through its nutritional evaluation. B. capriscus is characterized by high protein and low fat contents. High proportions of n-3 PUFA, mainly docosahexaenoic acid, were noted for the grey triggerfish. Nutritional properties of this species may be comparable to those of other lean fish species.
- Influências das produções televisivas para a experiência turística – o caso do Carnaval de Torres Vedras, PortugalPublication . Carvalho, Ana Paula dos Santos; López-Bonilla, Jesús Manuel; Almeida, António Sérgio Araújo deA pesquisa sobre os efeitos audiovisuais na experiência turística está muito focada nos comportamentos dos turistas. São ainda escassos os estudos sobre o papel da televisão no turismo e as estratégias económicas emancipadoras para o desenvolvimento local. Este estudo interpreta os efeitos das produções televisivas na experiência da visita ao Carnaval de Torres Vedras (Portugal), através da pesquisa de campo, análise documental e inquérito aos visitantes. O principal objetivo foi identificar um conjunto de fatores e atributos que possam contribuir para a afirmação turística e emancipação comunitária. Os resultados apontam para uma resposta positiva, ou seja, é possível que os efeitos das produções televisivas contribuam para a afirmação turística do Carnaval de Torres Vedras.