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- Broodstock conditioning of the Portuguese oyster (Crassostrea angulata, Lamarck, 1819): influence of different dietsPublication . Anjos, Catarina; Baptista, Teresa; Joaquim, Sandra; Mendes, Susana; Matias, Ana Margarete; Moura, Paula; Simões, Tiago; Matias, DomitíliaThe Portuguese oyster Crassostrea angulata shows great potential in oyster farming. The conservation of pure populations of this species is important for production diversification and biodiversity preservation. In this way, the zootechnological development for seed hatchery production is extremely important. Broodstock conditioning is a key step in the process of rearing bivalves in a hatchery. Many factors regulate the reproductive cycle, being food one of the most important ones. To evaluate the effect of different diets on C. angulata reproductive performance, broodstock were conditioned with different food regimes formulated fundamentally by flagellates (Diet 1 – Pavlova lutheri and Isochrysis galbana clone T-ISO; Diet 2 – P. lutheri, T-ISO and Skeletonema costatum) and constituted fundamentally by diatoms (Diet 3 – S. costatum and Chaetoceros calcitrans; Diet 4 – P. lutheri, S. costatum and C. calcitrans). During conditioning, samples of oysters were collected to evaluate condition index, gonadal development and biochemical composition. At the end of the conditioning period, oysters were induced to spawn to evaluate reproductive output (fecundity, fertilization rate and D-larvae development). The diets had an impact on the gametogenesis process, energy storage and reproductive output performance, being the best results those obtained in broodstock fed with the diatoms-predominant diets. However, those fed with diets majority flagellates had an unsuccessful performance. Holistic approaches incorporating all results in this study reveal and reinforce the idea that the diatom species used presented the nutritional requirements to C. angulata broodstock, being essential in the conditioning phase.
- Dance and the (Digital) Archive: A Survey of the FieldPublication . Fernandes, Carla; Coelho, Sílvia Pinto; Vieira, Ana BigotteThis paper offers a conspectus of several online dance archives made in the context of the Portuguese research project TKB. The online searches we conducted from 2018 to the end of 2019 suggested four broad categories of resources for what one may call 'online dance archives'. Aiming to observe how dance resources are available on the internet, we made each category correspond to a different operation-to collect (to build up a collection), to accumulate (to gather almost random material), to store (to organize according to a set of rules), to assemble (to compose and curate material). And we posed the same set of questions: For each of these categories: What is the mission of the archive, who are its subjects and objects, and which community of users does it bring together? The outcome is both a general overview, and the possibility of a comparative approach. Our original motivation has been to survey and to analyse a sample of available online resources for dance documentation and/or archiving, in order to feed TKB future projects and experiences. Starting from the TKB project perspective, and aiming at categorizing the different approaches to storage, curation, ownership and availability reflected by those archival platforms, we finally identified three major challenges in the relation between dance and the digital archive: The question of access, the ontology of dance and archive-what it is, what it has been, and what 'dance and archiving' can become in the future-, and the 'Will to archive' (cf. Lepecki 2010). Each one of these challenges will eventually provoke new questions as to the future of the TKB project and of its team of researchers, and the nature of the work they may undertake.
- Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis LuteaPublication . Gangadhar, Katkam N.; Rodrigues, Maria João; Pereira, Hugo; Gaspar, Helena; Malcata, F. Xavier; Barreira, Luísa; Varela, JoãoTisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extracts exhibited in vitro cytotoxic activity against human hepatocarcinoma (HepG2) cells. However, so far, the compound(s) accountable for the observed bioactivity have not been identified. Therefore, the aim of this study was to isolate and identify the chemical component(s) responsible for the bioactivity observed. Bioassay-guided fractionation through a combination of silica-gel column chromatography, followed by preparative thin layer chromatography (PTLC), led to the isolation of two diastereomers of a monoterpenoid lactone, namely, loliolide (1) and epi-loliolide (2), isolated for the first time in this species. The structural elucidation of both compounds was carried out by GC-MS and 1D (1 H and13 C APT) and 2D (COSY, HMBC, HSQC-ed, and NOESY) NMR analysis. Both compounds significantly reduced the viability of HepG2 cells and were considerably less toxic towards a non-tumoral murine stromal (S17) cell line, although epi-loliolide was found to be more active than loliolide. © 2020 by the authors.
- Growth Performance after Agouti-Signaling Protein 1 (Asip1) Overexpression in Transgenic ZebrafishPublication . Godino-Gimeno, Alejandra; Sánchez, Elisa; Guillot, Raúl; Rocha, Ana; Angotzi, Anna Rita; Leal, Esther; Rotllant, Josep; Cerdá-Reverter, José MiguelThe melanocortin system is a key structure in the regulation of energy balance. Overexpression of inverse agonists, agouti-signaling protein (ASIP), and agouti-related protein (AGRP) results in increased food intake, linear growth, and body weight. ASIP regulates dorsal-ventral pigment polarity through melanocortin 1 receptor (MC1R) and overexpression induces obesity in mice by binding to central MC4R. Asip1 overexpression in transgenic zebrafish (asip1-Tg) enhances growth, yet experiments show fish overexpressing Asip1 do not develop obesity even under severe feeding regimes. Asip1-Tg fish do not need to eat more to grow larger and faster; thus, increased food efficiency can be observed. In addition, asip1-Tg fish reared at high density are able to grow far more than wild-type (WT) fish reared at low density, although asip1-Tg fish seem to be more sensitive to crowding stress than WT fish, thus making the melanocortin system a target for sustainable aquaculture, especially as the U.S. Food and Drug Association has recently approved transgenic fish trading.
- Employer Branding Applied to SMEs: A Pioneering Model Proposal for Attracting and Retaining TalentPublication . Monteiro, Beatriz; Santos, Vasco; Reis, Isabel; Sampaio, Marta Correia; Sousa, Bruno; Martinho, Filipa; Sousa, Maria José; Au-Yong-Oliveira, ManuelMost business enterprises are small and medium-sized enterprises (SMEs), and many of them are without a human resource and recruitment department. Thus, one of the challenges that organizations currently face is to find a strategy to retain and attract talent. To overcome this difficulty, enterprises must invest in employer branding policies and be aware of the factors that differentiate them from others when attracting employees. This study aims to develop an employer branding model applicable to SMEs, to increase and enhance the attraction and retention of talents. An exploratory approach based on a quantitative perspective was adopted to develop an employer branding model applied to SMEs, with two major reference employer branding models and frameworks used as the main support. The model of employer branding was applied to SMEs regarding four dimensions, whereby essential questions are asked, namely (1) organizational culture (e.g., do employees have a job description aligned with the corporate culture?), (2) company strategy (e.g., what is the strategy if business volume decreases?), (3) company reputation (e.g., how do you perceive and treat negative feedback?), and (4) reward systems (e.g., do you feel that your employees are motivated intrinsically or extrinsically or both?), ordered by layers based on a logical sequence. The new proposed model is expected to serve as a useful strategic tool and as a basis for attracting, retaining and managing talent, specifically in the SMEs context. This new model provides a set of strategic and competitiveness benefits for SMEs, while contributing to making enterprises more profitable. The model also contributes to SMEs having a better image and reputation, enabling them to stand out from others in the war for talent.
- Bioproducts from forest biomass II. Bioactive compounds from the steam-distillation by-products of Cupressus lusitanica Mill. and Cistus ladanifer L. wastesPublication . Tavares, Cláudia S.; Martins, Alice; Miguel, M. Graça; Carvalheiro, Florbela; Duarte, Luís C.; Gameiro, José A.; Figueiredo, A. Cristina; Roseiro, Luísa B.Obtaining essential oils and hydrolates from underutilized biomass is an economic and sustainable way for production of these high added-value bioproducts. However, this process still generates large amounts of residues as the by-products obtained during distillation, which can be a concern for the environment, but also adequate substrates for other applications. Considering this fact, the waste distilled by-products remaining after steam-distillation of underutilized biomass from Cupressus lusitanica and Cistus ladanifer, were evaluated as a natural source of other high value products with biological activities, namely, phenolic compounds. Thus, the remaining extracted solid residues (ESRs) were characterized and subject to further treatments by ultrasound-assisted extraction (UAE) with ethanol and 70 % acetone, in order to prepare phenolic-rich extracts thereof: ESRs(EtOH) and ESRs(70 % Ace). Together with the distiller condensation waters (DCWs), these extracts were characterized for their phenolic content (total phenols, tannins and flavonoids). Their antioxidant activity was also evaluated by different methodologies. The phenolic profile of DCWs, ESRs(EtOH) and ESRs(70 % Ace) from both waste species was obtained by capillary zone electrophoresis (CZE) and phenolic compounds were tentatively identified. Results obtained for C. lusitanica biomass are here disclosed for the first time. Generally, all samples revealed to be rich in phenolic compounds, being C. ladanifer biomass the one with higher phenolic content. DCWs presented values of 140 mgGAE/g for C. lusitanica and 210 mg GAE/g for C. ladanifer, from which ca. 60 % were tannins. Extracts obtained with 70 % acetone were the ones with the highest results, except for the antioxidant activity by xanthine oxidase and superoxide inhibition, which was higher in DCWs. Catechins were the major compounds found for both species, but gallocatechins and gallic acid were only identified in C. ladanifer. Hydroxycinnamic acid derivatives and salicylic acid were also identified in C. ladanifer, partly justifying the anti-inflammatory effect referred for this species.
- Identification of the main quinolone resistance determinant in Campylobacter jejuni and Campylobacter coli by MAMA-DEG PCRPublication . Hormeño, Lorena; Palomo, Gonzalo; Ugarte-Ruiz, María; Porrero, M. Concepción; Borge, Carmen; Vadillo, Santiago; Píriz, Segundo; Domínguez, Lucas; Campos, Maria Jorge; Quesada, AlbertoAmong zoonotic diseases, campylobacteriosis stands out as the major bacterial infection producing human gastroenteritis. Antimicrobial therapy, only recommended in critical cases, is challenged by resistance mechanisms that should be unambiguously detected for achievement of effective treatments. Quinolone (ciprofloxacin) resistance of Campylobacter jejuni and Campylobacter coli, the2main Campylobacter detected in humans, is conferred by the mutation gyrA C-257-T, which can be genotyped by several methods that require a previous identification of the pathogen species to circumvent the sequence polymorphism of the gene. A multiplex PCR, based on degenerated oligonucleotides, has been designed for unambiguous identification of the quinolone resistance determinant in Campylobacter spp. isolates. The method was verified with 249 Campylobacter strains isolated from humans (141 isolates) and from the 3 most important animal sources for this zoonosis: poultry (34 isolates), swine (38 isolates), and cattle (36 isolates). High resistance to ciprofloxacin, MIC above 4 μg/mL, linked to the mutated genotype predicted by MAMA-DEG PCR (mismatch amplification mutation assay PCR with degenerated primers) was found frequently among isolates from the different hosts.
- Role of Phenolics Extracting from Rosa canina L. on Meat Protein Oxidation During Frozen Storage and Beef Patties ProcessingPublication . Utrera, Mariana; Morcuende, David; Maneta Ganhão, Rui Manuel; Estévez, MarioRecent findings suggested that using frozen material for meat processing leads to products with increased protein oxidation rates and impaired quality traits. Therefore, the effects of frozen storage and the addition of a phenolic-rich dog rose extract (Rosa canina L.; RC), on lipid and protein oxidation, moisture losses, color stability, and hardness of beef patties were investigated. Protein oxidation was assessed by means of tryptophan loss and the formation of specific lysine oxidation products: α-aminoadipic semialdehyde (AAS), α-aminoadipic acid (AAA), and Schiff bases. Frozen storage increased proteins susceptibility towards oxidation during successive technological processes. The addition of the RC extract inhibited the formation of AAS, AAA, and had an antioxidant effect towards tryptophan oxidation, but promoted the formation of Schiff bases and incremented the hardness of beef patties. The antioxidant effect may be attributed to the phenolic compounds, mainly procyanidins, found on the RC extract. Further knowledge on the interactions between phenolics and proteins is needed to optimize the application of these antioxidants against meat protein oxidation.
- The efficacy of MS-222 as anaesthetic agent in four freshwater aquarium fish speciesPublication . Chambel, João; Pinho, Rúben; Sousa, Rita; Ferreira, Tânia; Baptista, Teresa; Severiano, Vera; MENDES, SUSANA; Pedrosa, RuiThe efficacy of anaesthetic tricaine methanesulfonate (MS-222) was evaluated in four freshwater aquarium fish species, Zebrafish (Danio rerio), Guppy (Poecilia reticulata), Discu (Symphysodon discus) and Green swordtail (Xiphophorus helleri). The correct dose of anaesthetic should induce the plane 4 of anaesthesia in less than 180 s, recovery in less than 300 s and must survive when exposed during 30 min to anaesthetic. Fishes were exposed to six concentrations of anaesthetic (75, 100, 125, 150, 200 and 250 mg L-1) and the time of fish reaching plane 4 of anaesthesia, post exposure recovery, and the percentage of survival when fish were subject to 30 min in the anaesthetic were recorded. The optimal doses varied according to the species: D. rerio - 75, 100 and 125 mg L-1, P. reticulata - 125, 150 and 200 mg L-1, S. discus - 75 and 100 mg L-1 and X. helleri - 125 and 150 mg L-1. The induction time generally decreased significantly with increasing concentration of MS-222 for all of the species evaluated. The recovery time had a tendency to increase with the increase of the MS-222 concentration for D. rerio, P. reticulata and S. discus. On the other hand, X. helleri recovery time decreased with the increase of MS-222 concentration. MS-222 proved to be effective in anaesthesia for all the freshwater ornamental species studied. The main results clearly show that the optimal dose to anesthetize is fish species dependent and it is completely wrong to extrapolate optimal anaesthetic concentrations between different species.
- Mono-specific facies of Parazoanthus axinellae in Luiz Saldanha Marine ParkPublication . Gomes-Pereira, J.N.; Vasco-Rodrigues, N.; Afonso, A.; Albuquerque, M.; Berecibar, E.; Bernardes, L.; Calado, A.; Castanheira, A.; Tojeira, I.; Tempera, Fernando; Dias, F.C.; Santos, R.S.; Campos, A.S.We report the first mono-specific facies ofParazoanthus axinellaefrom the western Portuguese coast. The habitat was recorded using a MiniRov Observer on a large outcrop at ca. 55–60 m in the Luiz Saldanha Marine Park. A method to estimate the maximum uncertainty of the ROV position is illustrated and the occurrence of the facies is discussed.