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- Country Performance Analysis of Swiss Tourism, Leisure and Hospitality Management ResearchPublication . Cardoso, Lucília; Araújo, Arthur Filipe; Lima Santos, Luís; Schegg, Roland; Breda, Zélia; Costa, CarlosBased on tradition and high standards, Swiss higher education in tourism and hospitality is ranked among the best in the world. Although scientific research is the foundation of a coun-try’s higher education system, the Swiss Tourism, Leisure and Hospitality Management Scientific Research (Swiss TL&HM-SR) has not yet been subject to a systematic analysis. This paper aims to fill this gap by assessing the Swiss TL&HM-SR performance, as well as identifying and discussing its most prominent topics. To this end, bibliometric data were gathered from the Scopus database and analyzed through a bibliometric mixed-method approach. Results provide a new performance indicator for the Swiss TL&HM-SR in this field of research, and show that innovation and sustainable destination management are particularly prominent topics within Swiss TL&HM-SR. In this context, contributions to these topics in particular are discussed in more detail. The findings provide useful insights for stakeholders aiming to improve sustainability performance through strategic management of destinations, as well as for researchers aiming to follow the latest trends, identify emerging topics and formulate more attractive projects for financing institutions. The study also provides a new and innovative methodological contribution, as it combines different methods of scientific research performance assessment, which can be further employed in other countries or knowledge areas.
- An in situ assessment of key biomarkers in limpets exposed to sewage pollutionPublication . Silva, Cátia SE; Novais, Sara; Gravato, Carlos; Simões, Tiago; Caramalho, Márcia; Neves, Janete; Tecelão, Carla; Rodrigues, Maria J.; Maranhão, Paulo; Lemos, Marco F. L.
- EditorialPublication . Novais, Sara C.; Maranhão, Paulo
- Portal of security and safety engineering as a tool to increase the sustainable development of higher education in security in the European Union and beyondPublication . Lovecek, Tomas; Ristvej, Jozef; Kampova, Katarina; Vaculík, Juraj; Gamboa, Roberto; Zagorecki, AdamIn this article we describe a project "Competency Based e-Portal of Security and Safety Engineering" - eSEC portal approved by The Education, Audio-visual and Culture Executive Agency (EACEA), developed in the period between 10/2009 and 10/2012. The eSEC portal is one of 10 centralised multilateral projects approved in 2009 by EACEA and only one with the University of Zilina as a coordinator, with total budget 356.138 Euros. The EACEA is responsible for the management of certain elements of the EU's programs in the fields of education and culture. The idea for the eSEC project originated from the demand to increase the quality of education in the field of security and safety. One of the key problems of the education is disparity between the knowledge received from educational institutions and the practical requirements. It is necessary to redefine how students are prepared for the challenges of the labour market: identifying competencies that are currently in demand and how it would be possible to link the educational systems of various institutions more efficiently. The aim of the eSEC project was to develop the competencies of students, teachers, researchers and professionals working in the field of security and safety, within the EU and the world. To achieve these objectives, an electronic portal was developed at www.esecportal.eu. The portal was launched in May 2012, hopefully starting the way to become an established and well known Internet portal on the security and safety education.
- Development of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tardaPublication . Pinto, Micaela Ferreira; Baptista, Teresa; Afonso, Clélia Correia NevesDisease assessment and management in cultured aquatic animals is a major concern in commercial aquaculture. Disease outbreaks have direct effects on fish production, causing serious economic losses in this industry. This can be overcome by early detection through molecular high sensitivity tools such as PCR (polymerase chain reaction). One of the most critical steps in the study of bacterial fish diseases is the precise identification of the infectious agent. Considering the damage that some bacteria can cause to fish and humans, the development of a rapid detection method for the four target species that demonstrates to be simple, accurate and low cost is an essential step for the prevention and early treatment of these diseases. Edwardsiella tarda (ACC 36.1), Vibrio harveyi (DSM 19023), Vibrio anguillarum (AQV 55.1) and Vibrio alginolyticus (CECT 521) were selected as targets of a multiplex PCR tool. The multiplex PCR reactions were performed in various reaction conditions, including different annealing temperatures (between 49 °C and 55 °C) and changes in the MgCl2 concentration from 2mMto 8 mM. Best results were obtained at 51 °C and MgCl2 concentration from 4mM to 6 mM. Primers were tested using purified DNA from the respective bacterial strains, yielding bands at expected sizes, which can be identified on agarose gels with clearness and without overlapping sizes. Results indicate that this multiplex PCR tool is suitable for the detection of target pathogens and may, in the future, have extended practical application in commercial aquaculture.
- Cleaning interactions of fishes at São Tomé Island, West AfricaPublication . Vasco Rodrigues, Nuno; Fontes, Jorge; Bertoncini, A.This study reports six new types of cleaning interactions among tropical reef fish species at São Tomé Island, and confirms previously reported interactions for this area. Spicara melanurus was recorded as a cleaner for the first time, and Abudefduf saxatilis was confirmed in this role, following reports from the Southwest Atlantic. Individuals belonging to the family Labridae were the most commonly-observed cleaners, involved in over 85% of recorded interactions. Juveniles of Bodianus speciosus played a key role as cleaners, interacting with at least seven client species. In addition, some of the client fishes, including Paranthias furcifer and Acanthurus monroviae, interacted with at least four cleaner fish species. All cleaner fish species reported in this study were facultative; obligate cleaners appear to be absent from this region.
- The influence of cadmium contamination and salinity on the survival, growth and phytoremediation capacity of the saltmarsh plant Salicornia ramosissimaPublication . Pedro, Carmen; Santos, Márcia S.S.; Ferreira, Susana; Gonçalves, Sílvia C.The major aim of this study was to evaluate the capacity of Salicornia ramosissima on Cadmium phytoremediation under distinct salinities and, consequently, the toxic effects on the plant’s development. A greenhouse experiment was performed, using two Cd concentrations (50 and 100 mg l 1) in different salinities (0, 5 and 10). Mortality and weight variation, observed at the end of the experiment, showed significant differences between some treatments, meaning that these variables were affected by the salinity and Cd concentrations. The highest Cd accumulation was detected in the roots, and decreased with the increase of salinity and Cd concentration. S. ramosissima is a potential candidate for Cd phytoremediation at salinities close to 0 and its capabilities in Cd phytoaccumulation and phytoestabilization proved to be quite interesting. The optimization of phytoremediation processes by S. ramosissima could turn possible the use of this plant in the recovery of contaminated ecosystems.
- ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in ArabidopsisPublication . Rodrigues, Américo; Adamo, Mattia; Crozet, Pierre; Margalha, Leonor; Confraria, Ana; Martinho, Cláudia; Elias, Alexandre; Rabissi, Agnese; Lumbreras, Victoria; González-Guzmán, Miguel; Antoni, Regina; Rodriguez, Pedro L.; Baena-González, ElenaPlant survival under environmental stress requires the integration of multiple signaling pathways into a coordinated response, but the molecular mechanisms underlying this integration are poorly understood. Stress-derived energy deprivation activates the Snf1-related protein kinases1 (SnRK1s), triggering a vast transcriptional and metabolic reprogramming that restores homeostasis and promotes tolerance to adverse conditions. Here, we show that two clade A type 2C protein phosphatases (PP2Cs), established repressors of the abscisic acid (ABA) hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation. Accordingly, SnRK1 repression is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpression, sugar hypersensitivity during early seedling development. Reporter gene assays and SnRK1 target gene expression analyses further demonstrate that PP2C inhibition by ABA results in SnRK1 activation, promoting SnRK1 signaling during stress and once the energy deficit subsides. Consistent with this, SnRK1 and ABA induce largely overlapping transcriptional responses. Hence, the PP2C hub allows the coordinated activation of ABA and energy signaling, strengthening the stress response through the cooperation of two key and complementary pathways.
- Talitrid and Tylid crustaceans bioecology as a tool to monitor and assess sandy beaches’ ecological quality conditionPublication . Gonçalves, Sílvia C.; Anastácio, Pedro M.; Marques, João C.In the last decades, the sharp increase of human activities on sandy beaches has introduced several detrimental impacts on these ecosystems, highlighting the importance of developing environmental quality assessments and sustainable management and protection plans for these environments. The study of key species at the population level represents an adequate and important approach to the ecosystem's ecological quality if the impacts of environmental disturbances, namely derived from human activities, are to be assessed. Talitrid and Tylid crustaceans often form abundant populations in temperate exposed sandy beaches, and might represent the bulk of the macrofaunal communities. In the present case study, the comparison of distinct Talitrid and Tylid populations on Atlantic (Portugal) and Mediterranean (Italy and Tunisia) beaches allowed to assess the adaptation of these animals on local and macro scale scenarios and evaluate the importance of bioecological studies as auxiliary tools in environmental monitoring and in the ecological quality assessment of sandy beaches. The results revealed that Talitrid and Tylid populations have a strong plasticity over geographic gradients and to local environments, presenting a high degree of variation on population ecology, namely latitudinal clines on several life history traits. Also, since the studies were performed on sandy beaches with similar degrees of exposure to wave action and low degrees of human disturbance, the present work may play a relevant role as reference knowledge in environmental monitoring studies and as an ecological quality assessment tool that might be used to evaluate the impacts of environmental disturbances on sandy beach ecosystems, namely from human origin.
- Cytotoxic effects of Ridolfia segetum (L.) Moris phytoproducts in cancer cellsPublication . Beeby, Ellie; Magalhães, Mariana; Lemos, Marco F. L.; Pires, Isabel M.; Cabral, CéliaEthnopharmacological relevance: The past few years have witnessed an increasing interest in essential oils (EOs) as potential therapeutic agents against a wide variety of pathologies, including cancer. EOs extracted from Ridolfia segetum (L.) Moris (R. segetum) are a clear example of a phytoproduct with therapeutic applications, as it is widely used in traditional medicine due to its antioxidant and anti-inflammatory properties, and these properties were already validated by previous studies. Although, it is well established that inflammation is a key hallmark of cancer, with a key role promoting tumorigenesis, and being chronic inflammation often associated with tumorigenic processes, there are no previous studies regarding the assessment of the antitumoural potential of R. segetum EOs. Aim of the study: The present study intends to be the first to evaluate the antitumoural proprieties of R. segetum EO phytoproducts in cancer cell models. Materials and methods: For this, R. segetum EOs were extracted from plants collected at either flowering (RS_Fl) or fruiting (RS_Fr) stage. The impact on proliferation and viability of treatment with R. segetum EO extracts was assessed using in vitro 2D and 3D models. Results: Both R. segetum EOs presented effective antiproliferative/viability effects, evidence noted by low IC50 values in 2D models, and significant reduction of spheroid size in 3D in vitro models. Mechanistically, treatment with R. segetum EOs was associated with an altered G1 (associated with p21 stabilisation), and subsequent induction of apoptosis. Conclusions: Overall, these results indicate that R. segetum EOs have potential as suitable antitumoural therapeutic agents.