Non-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxide

Granada, Luana; Morão, Inês F.C.; Lemos, Marco F. L.; Bossier, Peter; Novais, Sara C.

http://hdl.handle.net/10400.8/15222

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Abstract(s)

The rotifer Brachionus sp. is of great importance for aquaculture, as the reproduction cycle under rearing conditions of many economically important species larvae depends on the use of rotifers as first live feed. Establishing a protocol that results in an improved tolerance of rotifers to environmental stressors will allow for a more stable rotifer production. The exposure to non-lethal heat shocks (NLHS) already proved to enhance the tolerance, not only to heat stress, but also to other stressors in several aquatic species, by activating the heat shock response and epigenetic mechanisms. This study aimed to determine the potential of a single NLHS to induce tolerance to different abiotic stressors in two strains of B. koreanus (MRS10 and IBA3) and to evaluate possible molecular mechanisms involved in the achievement of increased tolerance to hydrogen peroxide induced by NLHS. Cross-tolerance was achieved for both strains, namely to high salinity, cadmium chloride, and hydrogen peroxide. Scale-up tests resulted in increased tolerance to hydrogen peroxide only for MRS10. During the exposure to this substance, heat-shocked MRS10 rotifers showed an up-regulation of genes related to oxidative stress response and histone modifications, increased production of HSP70, and higher levels of total acetylation of histone H3. A single NLHS proved to induce epigenetic effects when rotifers were exposed to other stressor later in life. However, further studies should elucidate if the NLHS conditions used in this study can yield a persistent outcome, allowing the establishment of tolerant rotifer strain lines and, consequently, a more stable production.

Keywords

Epigenetic effects Gene expression Histone modifications qPCR Swimming capacity

URI

http://hdl.handle.net/10400.8/15222

Citation

Luana Granada, Inês F.C. Morão, Marco F.L. Lemos, Peter Bossier, Sara C. Novais, Non-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxide, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Volume 300, 2026, 110389, ISSN 1532-0456, https://doi.org/10.1016/j.cbpc.2025.110389.