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- Acute effects of dynamic stretching on knee joint position sense and dynamic balance in recreational runners: A randomized controlled trialPublication . Simões, Edgar; Tavares, Nuno; Saraiva, MarinaObjectives: Proprioception and dynamic balance are crucial elements of neuromuscular control during running, supporting movement precision and postural adjustments. Dynamic stretching is commonly used in warm-ups to enhance muscle activation and sensorimotor readiness, but its acute effects on proprioception and balance remain unclear. Design: Randomized controlled trial. Participants: Sixty-two healthy recreational runners (25–45 years; ≥20 km/week), randomly assigned to a Dynamic Stretching group (n = 31) or a control group performing light walking (n = 31). Main outcome measures: Joint position sense was assessed through active joint repositioning using 2D video analysis, with Absolute Angular Error, Relative Angular Error, and Variable Angular Error as outcomes. Balance was evaluated using the Y-Balance Test, including anterior, posteromedial, and posterolateral reach directions, as well as a composite score. Results: The Dynamic Stretching group showed significant reductions in Absolute Angular Error (p < 0.05) and Variable Angular Error (p < 0.001), with a between-group difference in Relative Angular Error (p = 0.043). Both groups improved Y-Balance Test scores, but the Dynamic stretching group achieved significantly greater gains in posteromedial reach and Composite Score (p < 0.001). Conclusions: Dynamic stretching acutely improves proprioceptive accuracy and dynamic balance in recreational runners, supporting its inclusion in warm-up routines.
- Non-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxidePublication . Granada, Luana; Morão, Inês F.C.; Lemos, Marco F. L.; Bossier, Peter; Novais, Sara C.The rotifer Brachionus sp. is of great importance for aquaculture, as the reproduction cycle under rearing conditions of many economically important species larvae depends on the use of rotifers as first live feed. Establishing a protocol that results in an improved tolerance of rotifers to environmental stressors will allow for a more stable rotifer production. The exposure to non-lethal heat shocks (NLHS) already proved to enhance the tolerance, not only to heat stress, but also to other stressors in several aquatic species, by activating the heat shock response and epigenetic mechanisms. This study aimed to determine the potential of a single NLHS to induce tolerance to different abiotic stressors in two strains of B. koreanus (MRS10 and IBA3) and to evaluate possible molecular mechanisms involved in the achievement of increased tolerance to hydrogen peroxide induced by NLHS. Cross-tolerance was achieved for both strains, namely to high salinity, cadmium chloride, and hydrogen peroxide. Scale-up tests resulted in increased tolerance to hydrogen peroxide only for MRS10. During the exposure to this substance, heat-shocked MRS10 rotifers showed an up-regulation of genes related to oxidative stress response and histone modifications, increased production of HSP70, and higher levels of total acetylation of histone H3. A single NLHS proved to induce epigenetic effects when rotifers were exposed to other stressor later in life. However, further studies should elucidate if the NLHS conditions used in this study can yield a persistent outcome, allowing the establishment of tolerant rotifer strain lines and, consequently, a more stable production.
- Optimisation of Alginate Extraction and Characterisation of Polysaccharides from Brown Seaweed from the Portuguese CoastPublication . Mendes, Joana Corrêa; Valente, Joana F. A.; Sousa, Fani; Bernardino, Raul; Bernardino, Susana; Afonso, Clélia; Chagas, BárbaraAlginate is a widely used and versatile biopolymer with an ever-expanding range of applications in the pharmaceutical and biomedical industries. This highlights the importance of developing sustainable and renewable production sources. Conventional extraction methods, although effective, are often energy-intensive and rely on harsh chemicals. In this context, brown algae are a promising alternative due to their abundance and renewability. This study investigated the potential of Saccorhiza polyschides and Sargassum muticum as sources of sodium alginate (SA), thus optimising an extraction process that combines acid treatment with an alkaline step. The extracted biopolymers were characterised using FTIR, H-NMR, STA, SEM/EDX, viscosity measurements, dynamic light scattering, and spectrophotometric assays of residual polyphenols and proteins. The optimised extraction conditions produced yields above 20% of high-purity alginate. When compared with commercial SA, the extracted materials showed comparable quality while relying on a simplified, solvent-reduced protocol that improves process efficiency and reduces the environmental impact. These results demonstrate that S. polyschides and S. muticum are promising, locally available sources of high-quality sodium alginate, and that industrially relevant yields (>20%) can be achieved through an environmentally conscious two-step extraction process.
- Interactions Between BMI and Age on Fall Risk in Older AdultsPublication . Rodrigues, Filipe; Monteiro, Diogo; Monteiro, A. M.; Forte, PedroBackground/Objectives: The aging process is typically marked by a reduction in functional fitness, which heightens the likelihood of falling. Although obesity is established as a determinant of poor mobility, the interplay between excess weight and advanced age is still a topic of research interest. Therefore, this research investigated how age, sex, and Body Mass Index (BMI) interact to influence fall risk among older adults living in the community. Methods: This cross-sectional investigation involved 815 participants (Mage = 70.45 ± 6.10 years), stratified by age (sexagenarians, septuagenarians, octogenarians) and BMI (normal weight, overweight, obesity). Fall risk was assessed using the Timed Up and Go test. A Three-Way ANOVA examined the main and interaction effects. Results: No significant three-way interaction (p = 0.334) or main effect of sex (p = 0.079) was found. However, a significant age x BMI interaction was observed (p = 0.007). In sexagenarians and septuagenarians, obesity was associated with significantly slower fall risk performance compared to normal weight (p < 0.001). Conversely, in octogenarians, this difference was not significant (p = 1.000) with normal-weight individuals. Conclusions: Obesity may be a significant risk factor for falls, especially in adults aged 60 to 79 years. In octogenarians, the protective benefit of normal weight disappears, revealing a “weight paradox” likely driven by sarcopenia. Fall risk assessments and weight management strategies should be tailored to age, focusing on preserving muscle mass in octogenarians.
- Short-Term 8-Foot up and Go Responsiveness in Institutionalized vs. Community-Dwelling Older AdultsPublication . Rodrigues, Filipe; Monteiro, Diogo; Forte, Pedro; Monteiro, A. M.This study aimed to examine the combined effects of Body Mass Index (BMI) and living setting on fall risk trajectories in older adults over a 16-week period. A total of 124 older adults were recruited from nursing homes (n = 65; Mage: 84.4 ± 7.7 years) and community settings (n = 59; Mage: 70.3 ± 3.6 years). Participants were stratified by BMI (normal weight, overweight, and obesity) and assessed for functional mobility using the 8-foot Timed Up and Go test at baseline and after 16 weeks. A 3 × 2 × 2 repeated-measures GLM using the multivariate approach (Pillai’s Trace) revealed significant main effects for time (p < 0.001) and Living Setting (p < 0.001), but not for BMI (p = 0.408). A significant time × living setting interaction (p < 0.001) indicated that institutionalized older adults demonstrated a significant reduction in 8-foot Up-and-Go completion time (~16%), whereas community-dwelling peers maintained their baseline levels. These findings suggest that the observed reduction in time reflects a test familiarization effect rather than physiological improvement. Analysis revealed that the living setting profile was strongly associated with performance, showing a more significant link to functional decline than BMI-defined weight status, as no significant differences were found across BMI categories.
- Mapping the Second Victim Experience Among Western Nurses: A Scoping ReviewPublication . Costeira, Cristina; Junqueira, Helena; Quintas, Pedro; Pragosa, Ângela; Mata, Ema; Duarte, Hugo; Bom, Luís; Pais, NelsonBackground/Objectives: The second victim phenomenon is increasingly recognized as a significant issue affecting nurses involved in adverse events resulting from clinical decisions or interventions. Although patients and families, considered the first victims, are directly impacted, nurses often undergo challenges as second victims. With the growing awareness of these effects, this study aimed to map recent evidence on the second victim phenomenon among nurses in Western countries. Methodology: A Scoping Review was conducted following the Joanna Briggs Institute methodology in September 2024 and updated in November 2025. Eligibility criteria were defined using the PCC (Population, Concept, Context) framework. Searches were performed in PubMed, CINAHL, SciELO, and Scopus. Two independent reviewers carried out study selection, data extraction, and synthesis. Rayyan® supported screening, performed in two phases: title/abstract review and full-text analysis. Data extraction was conducted in Excel®, and data were analyzed using descriptive statistics and categorized into thematic areas. The review followed PRISMA-ScR guidelines and was registered in the Open Science Framework. Results: Of the 111 articles retrieved, 39 met the inclusion criteria. Evidence shows that although several support programs exist for nurses as second victims, they are often perceived as inadequate or inconsistently implemented. Second victim experience is associated with physical (e.g., sleep disturbances), emotional (e.g., fear), and psychological (e.g., distress) symptoms, with consequences such as absenteeism, professional dissatisfaction, loss of meaning in life, and even suicide. Conclusion: Findings highlight the need for more comprehensive, accessible, and consistently implemented support strategies to meet the complex needs of nurses affected by the second victim phenomenon.
- Self-selected Recovery in High-intensity Interval Training Promotes Positive Responses in Affect, Pleasure, and Mood in Young PeoplePublication . Sant’Ana, Leandro; Vianna, Jeferson Macedo; Travassos, Bruno; Nakamura, Fábio Yuzo; Teixeira, Diogo; Scartoni, Fabiana Rodrigues; Dias, Amandio; Antunes, Raul; Rodrigues, Filipe; Matos, Rui; Machado, Sérgio; Monteiro, DiogoIntroduction: High-Intensity Interval Training (HIIT) with self-selected recovery can promote positive emotional responses. This study aimed to verify the acute responses to HIIT with fixed recovery and to select high scorers on the state of affect, enjoyment, and mood in healthy young people. Methods: Nineteen participants took part in the study (19.0±1.0 years, 64.0±9.2 kg, 169.1±8.5, and 22.0±2.0 BMI). They performed 10 x 30 s (95% V peak ) with 1 min recovery (HIIT RecA - active - 40% V peak ) and self-selected (HIIT Rec-B ). Affect, enjoyment, and mood were assessed before and after HIIT (both conditions). Results: In both conditions, for time 1 and 2 were positive in positive ( p =.170, EF: .102) and negative ( p =.0.90, EF: .151) affect, pleasure ( p =.010, EF: .315), and mood domains (tension: p <.001, EF: .673; depression: p =.015, EF: .286), anger: p =.033, EF: .230, and mental confusion: p <.001, EF: .451). In contrast, there was a reduction in the vigor domain and an increase in fatigue, with no differences in all emotional variables for condition and time*condition ( p >.05). Conclusion: Thus, selected self-recovery may be a strategy in prescribing HIIT with possibilities of not interfering with the emotional aspects, researched here.
- Practices and Challenges in Portuguese Early Childhood Intervention: A Descriptive StudyPublication . Costeira , Cristina; Lopes, Inês; Lopes, Saudade; Pedrosa, Vanda Varela; Custódio, Susana; Cioga, Elisabete; Silva, Cândida G.Background/Objectives: Early Childhood Intervention (ECI) services are critical for supporting children with developmental needs and their families. Despite an established legislative framework, challenges related to accessibility, equity, resources, and standardization of practices persist. This study aimed to describe the perspectives of early intervention professionals in Portugal regarding current barriers, facilitators, and priority areas for improvement within the system. Methods: A descriptive study was conducted involving 82 professionals working in early intervention in Portugal. Data were collected using a survey specifically developed by the research team, grounded in a comprehensive literature review and professional expertise. The instrument was validated through a Delphi Panel with two rounds involving six experts in ECI. Data from open-ended questions were analyzed using content analysis, identifying categories and sub-categories to describe the responses, and descriptive statistics for the closed-ended questions. Results: Professionals highlighted the need to update the National ECI System (SNIPI), improve accessibility, and ensure equitable access to early intervention services. Participants reported limited resources, a lack of standardization in practices, and emphasized the importance of professional training and continuous professional development. The findings also pointed to the urgent need for investment and functional and structural restructuring of early intervention services. Various barriers and facilitators were identified. Conclusions: The study provides valuable insights into the perspectives of early intervention professionals, identifying critical areas for policy improvement, resource allocation, and practice standardization.