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Effects of cadmium and resource quality on freshwater detritus processing chains: a microcosm approach with two insect species
Publication . Campos, Diana; Alves, Artur; Lemos, Marco F. L.; Correia, António; Soares, Amadeu M. V. M.; Pestana, João L. T.
Detritus processing is vital for freshwater ecosystems that depend on the leaf litter from riparian vegetation and is mediated by microorganisms and aquatic invertebrates. Shredder invertebrates transform coarse particulate organic matter into fine particulate organic matter used as food by collector species. Direct and indirect effects of contaminants can impair detritus processing and thus affect the functioning of these ecosystems. Here, we assessed the combined effects of a toxic metal (cadmium) and resource quality (leaf species) on detritus processing and shredder-collector interactions. We considered two types of leaves, alder and eucalyptus that were microbially conditioned under different Cd concentrations in the laboratory. The microbial communities present on leaves were analyzed by Denaturing Gradient Gel Electrophoresis (DGGE), and we also measured microbial respiration rates. Sericostoma vittatum (a caddisfly shredder) and Chironomus riparius (a midge collector) were also exposed to Cd and allowed to consume the corresponding alder or eucalyptus leaves. We evaluated C. riparius growth and leaf mass loss in multispecies microcosms. Cadmium exposure affected leaf conditioning and fungal diversity on both leaf species, as assessed by DGGE. Cadmium exposure also affected the mass loss of alder leaves by reductions in detritivore feeding, and impaired C. riparius growth. Chironomus riparius consumed alder leaf discs in the absence of shredders, but S. vittatum appear to promote C. riparius growth in treatments containing eucalyptus. These results show that indirect effects of contaminants along detritus-processing chains can occur through effects on shredder-collector interactions such as facilitation but they also depend on the nutritional quality of detritus and on sensitivity and feeding plasticity of detritivore species.
Design of tissue engineering scaffolds based on hyperbolic surfaces: Structural numerical evaluation
Publication . Almeida, Henrique A.; Bártolo, Paulo J.
Tissue engineering represents a new field aiming at developing biological substitutes to restore, maintain, or improve tissue functions. In this approach, scaffolds provide a temporary mechanical and vascular support for tissue regeneration while tissue in-growth is being formed. These scaffolds must be biocompatible, biodegradable, with appropriate porosity, pore structure and distribution, and optimal vascularization with both surface and structural compatibility. The challenge is to establish a proper balance between porosity and mechanical performance of scaffolds.This work investigates the use of two different types of triple periodic minimal surfaces, Schwarz and Schoen, in order to design better biomimetic scaffolds with high surface-to-volume ratio, high porosity and good mechanical properties. The mechanical behaviour of these structures is assessed through the finite element method software Abaqus. The effect of two parametric parameters (thickness and surface radius) is also evaluated regarding its porosity and mechanical behaviour.
Life satisfaction in young footballers: The contribution of basic needs satisfaction and passion
Publication . Braz, Diogo Salgado; Gouveia, Élvio R; Teixeira, Diogo S; Sarmento, Hugo; Monteiro, Diogo
The present study was grounded in Self-Determination Theory and the Dualistic Model of Passion. The objective of the study was to examine the relationships between basic psychological need satisfaction, passion (harmonious and obsessive), and life satisfaction in youth football players. The sample comprised 403 male youth football players aged 13 to 17 years who completed validated self-report measures assessing autonomy, competence, relatedness, passion for football, and global life satisfaction. The structural equation modeling approach was employed to assess direct and indirect
associations among variables, as well as to ascertain invariance of the model across age groups. The results of the study indicated that the satisfaction of basic psychological needs was associated with a propensity toward harmonious passion, while concurrently demonstrating an inverse relationship with obsessive passion. The harmonious passion–life satisfaction relationship was found to be positive, while the obsessive passion–life satisfaction relationship was found to be negative. The present study examined the relationship between need satisfaction and life satisfaction, with the hypothesis that passion would mediate this relationship. It was predicted that the way players internalize their motivation would play a central role in translating sport experiences into broader well-being. The structural model demonstrated invariance across age groups, thereby suggesting developmental robustness of these motivational processes throughout adolescence. These findings underscore the significance of need-supportive environments in youth football, which are instrumental in fostering adaptive forms of passion and promoting long-term psychological well-being.
A stochastic approach to optimize Maritime pine (Pinus pinaster Ait.) stand management scheduling under fire risk. An application in Portugal
Publication . Ferreira, L.; Constantino, M.; Borges, J. G.
The paper discusses research aiming at the development of a management scheduling model for even-aged stands that may take into consideration fuel treatments to address the risk of wildfires. A Stochastic dynamic programming (SDP) approach is proposed to determine the policy (e.g. the fuel treatment and thinning schedules and the rotation age) that produces the maximum expected discounted net revenue. Fuel treatment activities encompass shrub cleanings. Emphasis was on combining a deterministic stand-level growth and yield model with wildfire occurrence and damage models to design a SDP network. SDP stages are defined by age and state variables include both the stand basal area and the number of years since the last fuel treatment. Fire occurrence and damage scenarios are addressed at each stage. Results from an application to Maritime pine (Pinus pinaster Ait.) stand management scheduling in Leiria National Forest, Portugal, are presented. Results suggest that the modeling strategy may help assess the impact of wildfire risk on the optimal stand management schedule. They confirm that the maximum expected discounted net revenues decreases. Further, albeit some timber may be salvaged after the wildfire, rotation age also decreases when the risk of fire is considered. Finally, they provide interesting insights about the role of thinning and fuel treatment policies in mitigating risk.
Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly: In Vitro and In Vivo Analysis
Publication . Pereira, T.; Gärtner, A.; Amorim, I.; Almeida, A.; Caseiro, A.R.; Armada-da-Silva, Paulo A. S.; Amado, Sandra; Fregnan, Federica; Varejão, A. S. P.; Santos, J. D.; Bártolo, P. J.; Geuna, S.; Luís, A. L.; Maurício, A. C.
In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-ε-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Resultsin vitroshowed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed.In vivotesting evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery.