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Publication
Natural approaches for neurological disorders: The neuroprotective potential of Codium tomentosum
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Advisor(s)
Abstract(s)
Parkinson’s disease (PD) is the second most common neurodegenerative disorder, and is characterized by a progressive degeneration of the dopaminergic neurons in the substantia nigra. Although not completely understood, several abnormal cellular events are known to be related with PD progression, such as oxidative stress, mitochondrial dysfunction and apoptosis. Accordingly, the aim of this study was to evaluate the neuroprotective e ects of Codium tomentosum enriched fractions in a neurotoxicity model mediated by 6-hydroxydopamine (6-OHDA) on SH-SY5Y human cells, and the disclosure of their mechanisms of action. Additionally, a preliminary chemical screening of the most promising bioactive fractions of C. tomentosum was carried out by GC-MS analysis. Among the tested fractions, four samples exhibited the capacity to revert the neurotoxicity induced by 6-OHDA to values higher or similar to the vitamin E (90.11 +- 3.74% of viable cells). The neuroprotective e ects were mediated by the mitigation of reactive oxygen species (ROS) generation, mitochondrial dysfunctions and DNA damage, together with the reduction of Caspase-3 activity. Compounds
belonging to different chemical classes, such as terpenes, alcohols, carboxylic acids, aldehydes, esters,
ketones, saturated and unsaturated hydrocarbons were tentatively identified by GC-MS. The results
show that C. tomentosum is a relevant source of neuroprotective agents, with particular interest for
preventive therapeutics.
Description
Funding: This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the strategic project UID/MAR/04292/2020 to MARE—Marine and Environmental Sciences Centre and UID/Multi/04046/2020 and UIDB/04046/2020 granted to BioISI—BioSystems and Integrative Sciences Institute, through POINT4PAC project (Oncologia de Precisão: Terapias e Tecnologias Inovadoras, SAICTPAC/0019/2015-LISBOA- 01-0145-FEDER-016405), through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017),
co-financed by COMPETE (POCI-01-0145-FEDER-029250) and through Molecules for Health project (PTDC/BIA-BQM/28355/2017). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund.
Acknowledgments: The authors are very grateful for the financial support of the Projects and Programmes described in the funding section. FCT is also acknowledged for the grants attributed to JS (SFRH/BD/103255/2014), and SP (SFRH/BD/96203/2013).
Acknowledgments: The authors are very grateful for the financial support of the Projects and Programmes described in the funding section. FCT is also acknowledged for the grants attributed to JS (SFRH/BD/103255/2014), and SP (SFRH/BD/96203/2013).
Keywords
Seaweed Marine natural products Neurodegenerative disease SH-SY5S Oxidative stress Mitochondrial dysfunction
Citation
Silva, J.; Martins, A.; Alves, C.; Pinteus, S.; Gaspar, H.; Alfonso, A.; Pedrosa, R. Natural Approaches for Neurological Disorders—The Neuroprotective Potential of Codium tomentosum. Molecules 2020, 25, 5478. https://doi.org/10.3390/molecules25225478
Publisher
MDPI