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Non-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxide

2026-02Journal article Open access
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorGranada, Luana
dc.contributor.authorMorão, Inês F.C.
dc.contributor.authorLemos, Marco F. L.
dc.contributor.authorBossier, Peter
dc.contributor.authorNovais, Sara C.
dc.date.accessioned2026-01-05T15:16:08Z
dc.date.available2026-01-05T15:16:08Z
dc.date.issued2026-02
dc.description.abstractThe rotifer Brachionus sp. is of great importance for aquaculture, as the reproduction cycle under rearing conditions of many economically important species larvae depends on the use of rotifers as first live feed. Establishing a protocol that results in an improved tolerance of rotifers to environmental stressors will allow for a more stable rotifer production. The exposure to non-lethal heat shocks (NLHS) already proved to enhance the tolerance, not only to heat stress, but also to other stressors in several aquatic species, by activating the heat shock response and epigenetic mechanisms. This study aimed to determine the potential of a single NLHS to induce tolerance to different abiotic stressors in two strains of B. koreanus (MRS10 and IBA3) and to evaluate possible molecular mechanisms involved in the achievement of increased tolerance to hydrogen peroxide induced by NLHS. Cross-tolerance was achieved for both strains, namely to high salinity, cadmium chloride, and hydrogen peroxide. Scale-up tests resulted in increased tolerance to hydrogen peroxide only for MRS10. During the exposure to this substance, heat-shocked MRS10 rotifers showed an up-regulation of genes related to oxidative stress response and histone modifications, increased production of HSP70, and higher levels of total acetylation of histone H3. A single NLHS proved to induce epigenetic effects when rotifers were exposed to other stressor later in life. However, further studies should elucidate if the NLHS conditions used in this study can yield a persistent outcome, allowing the establishment of tolerant rotifer strain lines and, consequently, a more stable production.eng
dc.description.sponsorshipThis study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project granted to MARE – Marine and Environmental Sciences Centre (UIDB/04292/2020 and UIDP/04292/ 2020) and the project granted to the Associate Laboratory ARNET (LA/ P/0069/2020). Luana Granada also wishes to acknowledge the financial support given by FCT (SFRH/BD/102036/2014). Sara Novais is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July.
dc.identifier.citationLuana Granada, Inês F.C. Morão, Marco F.L. Lemos, Peter Bossier, Sara C. Novais, Non-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxide, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Volume 300, 2026, 110389, ISSN 1532-0456, https://doi.org/10.1016/j.cbpc.2025.110389.
dc.identifier.doi10.1016/j.cbpc.2025.110389
dc.identifier.issn1532-0456
dc.identifier.urihttp://hdl.handle.net/10400.8/15222
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationMarine and Environmental Sciences Centre
dc.relationAquatic Research Infrastructure Network
dc.relationEpiStress - Epigenetic mechanisms of stress tolerance in Nereis diversicolor
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1532045625002704
dc.relation.ispartofComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEpigenetic effects
dc.subjectGene expression
dc.subjectHistone modifications
dc.subjectqPCR
dc.subjectSwimming capacity
dc.titleNon-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxideeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleAquatic Research Infrastructure Network
oaire.awardTitleEpiStress - Epigenetic mechanisms of stress tolerance in Nereis diversicolor
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0069%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F102036%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F04292%2F2020/PT
oaire.citation.titleComparative Biochemistry and Physiology Part C: Toxicology and Pharmacology
oaire.citation.volume300
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
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person.givenNameLuana
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person.givenNameSara
person.identifier996337
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person.identifier.scopus-author-id56513933800
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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