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Multidrug resistance assessment of indoor air in Portuguese long-term and acute healthcare settings

2025-05Research article Open access
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg17:Parcerias para a Implementação dos Objetivos
dc.contributor.authorSantos-Marques, C.
dc.contributor.authorTeixeira, C.
dc.contributor.authorPinheiro, R.
dc.contributor.authorBrück, W. M.
dc.contributor.authorPereira, Sónia Gonçalves
dc.contributor.authordos Santos Marques, Catarina
dc.contributor.authorSilva Teixeira, Camila
dc.contributor.authorPinheiro, Rafael
dc.contributor.authorGonçalves Pereira, Sónia
dc.date.accessioned2025-04-16T13:50:17Z
dc.date.available2025-04-16T13:50:17Z
dc.date.issued2025-05
dc.date.updated2025-04-13T17:21:22Z
dc.description.abstractBackground: Knowledge about air as a pool of pathogens and multidrug resistance (MDR) in healthcare units apart from hospitals is scarce. Aim: To investigate these features in a Portuguese long-term healthcare unit (LTHU) and a central hospital (CH). Methods: Air samples were collected and their microbial load (bacteria and fungi) determined. Bacterial isolates were randomly selected for further characterization, particularly identification by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, antimicrobial susceptibility testing, and polymerase chain reaction screening of extended-spectrum b-lactamases, carbapenemase genes and mecA gene, with RAPD profile assessment of positive results of the latter. Findings: A total of 192 samples were collected (LTHU: 86; CH: 106). LTHU showed a statistically significantly higher bacterial load. CH bacteria and fungi loads in inpatient sites were statistically significantly lower than in outpatients or non-patient sites. A total of 164 bacterial isolates were identified (MALDI-TOF: 78; presumptively: 86), the majority belonging to Staphylococcus genus (LTHU: 42; CH: 57). The highest antimicrobial resistance rate was to erythromycin and vancomycin the least, in both settings. Eighteen isolates (11%) were classified as MDR (LTHU: 9; CH: 9), with 7 MDR Staphylococcus isolates (LTHU: 4; CH: 3) presenting mecA. Nine non-MDR Staphylococcus (LTHU: 5; CH: 4) also presented mecA. Conclusion: The current study highlights that healthcare unit indoor air can be an important pool of MDR pathogens and antimicrobial resistance genes. Also, LTHUs appear to have poorer air quality than hospitals, as well as supportive areas compared to curative care areas. This may suggest possible yet unknown routes of infection that need to be explored.por
dc.description.sponsorshipTThis work was supported by CCISP-HES.SO Seed Money funding, by Fundação para a Ciência e Tecnologia (FCT), Portugal, and University of Applied Sciences and Arts Western Switzerland (HES-SO), Switzerland (Project n. 105214). S.G.P. also acknowledges her direct funding from FCT (CEECINST/00051/2018; https://doi.org/10.54499/CEECINST/00051/2018/CP1566/CT0004), as well as all ciTechCare based authors for FCT funding for their research unit (UIDB/05704/2020; https://doi.org/10.54499/UIDB/05704/2020
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSantos-Marques, C., C. Teixeira, R. Pinheiro & et al. (2025). Multidrug resistance assessment of indoor air in Portuguese long-term and acute healthcare settings. Journal of Hospital Infection, 159, 115 - 123. https://doi.org/10.1016/j.jhin.2025.02.005
dc.identifier.doi10.1016/j.jhin.2025.02.005en_US
dc.identifier.eissn1532-2939
dc.identifier.issn0195-6701
dc.identifier.slugcv-prod-4376181
dc.identifier.urihttp://hdl.handle.net/10400.8/12810
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCenter for Innovative Care and Health Technology
dc.relationUIDB/05704/2020
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0195670125000362
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectInfection prevention and control
dc.subjectMultidrug resistance
dc.subjectAir quality
dc.titleMultidrug resistance assessment of indoor air in Portuguese long-term and acute healthcare settingsen_US
dc.typeresearch articleen_US
dspace.entity.typePublication
oaire.awardTitleCenter for Innovative Care and Health Technology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05704%2F2020/PT
oaire.citation.endPage123
oaire.citation.startPage115
oaire.citation.titleJournal of Hospital Infectionen_US
oaire.citation.volume159
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamedos Santos Marques
person.familyNameSilva Teixeira
person.familyNamePinheiro
person.familyNameGonçalves Pereira
person.givenNameCatarina
person.givenNameCamila
person.givenNameRafael
person.givenNameSónia
person.identifier461252
person.identifier.ciencia-id6B1D-196D-1238
person.identifier.ciencia-id8513-F117-D554
person.identifier.ciencia-idEB1F-4DB2-B0C9
person.identifier.orcid0000-0002-3460-2275
person.identifier.orcid0000-0003-0599-4051
person.identifier.orcid0000-0002-2369-9016
person.identifier.orcid0000-0001-8197-1850
person.identifier.ridM-7588-2017
person.identifier.scopus-author-id57204116615
person.identifier.scopus-author-id7202024712
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid8615-92D1-7858 | Sónia Gonçalves Pereira
rcaap.rightsopenAccessen_US
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relation.isAuthorOfPublication564475e9-243b-4637-8504-709253dd1239
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