ESTG - Artigos em revistas internacionais
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Browsing ESTG - Artigos em revistas internacionais by Field of Science and Technology (FOS) "Ciências Agrárias::Biotecnologia Agrária e Alimentar"
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- 2-Hydroxy-4-methoxybenzaldehyde fromHemidesmus indicusis antagonistic toStaphylococcus epidermidisbiofilm formationPublication . Kannappan, Arunachalam; Durgadevi, Ravindran; Srinivasan, Ramanathan; Lagoa, Ricardo José Lucas; Packiavathy, Issac Abraham Sybiya Vasantha; Pandian, Shunmugiah Karutha; Ravi, Arumugam VeeraStaphylococcus epidermidis (SE) is an opportunistic nosocomial pathogen that accounts for recalcitrant device-related infections worldwide. Owing to the growing interest in plants and their secondary metabolites targeting bacterial adhesion, this study was intended to uncover the anti-biofilm potential of Hemidesmus indicus and its major constituent 2-hydroxy-4-methoxybenzaldehyde (HMB) against SE. The minimum biofilm inhibitory concentration (MBIC) of H. indicus root extract and HMB were found to be 500 and 250 µg ml−1, respectively. The results of time-dependent biofilm inhibition and mature biofilm disruption assays confirmed that HMB targets initial cell adhesion. Furthermore, interference by HMB in the expression of adhesin genes (icaA, aap and bhp) and biofilm components was associated with an increased susceptibility of SE to oxidative stress and antibiotics. To conclude, this study reports for the first time HMB as a potential drug against SE biofilms.
- Production of parsley and pennyroyal with an African catfish-based aquaponics partially fed with yellow mealworms - Tenebrio molitorPublication . Bernardino, Raul; Vieira, Judite; Vaz, Daniela C.; Santos, Ounísia D.; Ribeiro, Vânia S.; Pires, Cristiana L.; Cotrim, Luís; Bernardino, SusanaInsects can be used as alternative protein/food sources. Here, a novel aquaponic system based on the African catfish (Clarias gariepinus) fed with 30% of yellow mealworms (YM) (Tenebrio molitor) as substitute feed to 100% of fish meal (FM) was employed to produce parsley (Petroselinum crispum) and pennyroyal (Mentha pulegium). The two systems implemented (30YW/70FM and 100FM - control) showed identical water quality parameters. The 30YW/70FM operation led to a reduction by 27% of the carbon footprint, relatively to the 100FM system. Plants cultivated in the 30YW/70FM aquaponic system led to lower plant biomass (↓∼75%), as stated by the statistically significant (p < 0.05) lower values of “plant height”, “foliage diameter”, “leaf number”, “biggest leaf length” and “root length”. Moreover, 100FM parsley showed higher levels of greenness (100%) and health status (97%) than the 30YW/70FM plants (↓50% greenness; ↓20% health status). Likewise, 100FM pennyroyal also showed higher levels of greenness (100%) and health status (100%) than 30YW/70FM pennyroyal (↓56 % greenness; ↓59% health status). Also, even if all plants presented equivalent levels of P, K, Ca and Fe, the plants grown in the 30YW/70FM system showed some Cl-accumulation (>7.0 g Cl.kg−1 for parsley and > 4.0 g Cl.kg−1 for pennyroyal, in dry weight) in the leaves and Cu and Mn accumulation in the roots (> 0.4 g.kg−1 in dry weight). The presence of high concentrations of Cl in T. molitor, and consequently in the water and fish faeces, might have caused some abiotic stress and toxicity to plant tissues, reducing plant growth.
