2-Hydroxy-4-methoxybenzaldehyde from<i>Hemidesmus indicus</i>is antagonistic to<i>Staphylococcus epidermidis</i>biofilm formation

Kannappan, Arunachalam; Durgadevi, Ravindran; Srinivasan, Ramanathan; Lagoa, Ricardo José Lucas; Packiavathy, Issac Abraham Sybiya Vasantha; Pandian, Shunmugiah Karutha; Ravi, Arumugam Veera

http://hdl.handle.net/10400.8/14091

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2-Hydroxy-4-methoxybenzaldehyde from Hemidesmus indicus is antagonistic to Staphylococcus epidermidis biofilm formation.pdf	Staphylococcus epidermidis (SE) is an opportunistic nosocomial pathogen that accounts for recalcitrant device-related infections worldwide. Owing to the growing interest in plants and their secondary metabolites targeting bacterial adhesion, this study was intended to uncover the anti-biofilm potential of Hemidesmus indicus and its major constituent 2-hydroxy-4-methoxybenzaldehyde (HMB) against SE. The minimum biofilm inhibitory concentration (MBIC) of H. indicus root extract and HMB were found to be 500 and 250 µg ml−1, respectively. The results of time-dependent biofilm inhibition and mature biofilm disruption assays confirmed that HMB targets initial cell adhesion. Furthermore, interference by HMB in the expression of adhesin genes (icaA, aap and bhp) and biofilm components was associated with an increased susceptibility of SE to oxidative stress and antibiotics. To conclude, this study reports for the first time HMB as a potential drug against SE biofilms.	3.57 MB	Adobe PDF	Download

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Authors

Kannappan, Arunachalam

Durgadevi, Ravindran

Srinivasan, Ramanathan

Lagoa, Ricardo José Lucas

Packiavathy, Issac Abraham Sybiya Vasantha

Pandian, Shunmugiah Karutha

Ravi, Arumugam Veera

Abstract(s)

Staphylococcus epidermidis (SE) is an opportunistic nosocomial pathogen that accounts for recalcitrant device-related infections worldwide. Owing to the growing interest in plants and their secondary metabolites targeting bacterial adhesion, this study was intended to uncover the anti-biofilm potential of Hemidesmus indicus and its major constituent 2-hydroxy-4-methoxybenzaldehyde (HMB) against SE. The minimum biofilm inhibitory concentration (MBIC) of H. indicus root extract and HMB were found to be 500 and 250 µg ml−1, respectively. The results of time-dependent biofilm inhibition and mature biofilm disruption assays confirmed that HMB targets initial cell adhesion. Furthermore, interference by HMB in the expression of adhesin genes (icaA, aap and bhp) and biofilm components was associated with an increased susceptibility of SE to oxidative stress and antibiotics. To conclude, this study reports for the first time HMB as a potential drug against SE biofilms.

Keywords

Anti-biofilm Hemidesmus indicus 2-hydroxy-4-methoxybenzaldehyde Staphylococcus epidermidis

URI

http://hdl.handle.net/10400.8/14091

Citation

Kannappan, A., Durgadevi, R., Srinivasan, R., Lagoa, R. J. L., Packiavathy, I. A. S. V., Pandian, S. K., & Veera Ravi, A. (2020). 2-Hydroxy-4-methoxybenzaldehyde from Hemidesmus indicus is antagonistic to Staphylococcus epidermidis biofilm formation. Biofouling, 36(5), 549–563. https://doi.org/10.1080/08927014.2020.1777989.