Browsing by Author "Pereira, Hugo"
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- Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis LuteaPublication . Gangadhar, Katkam N.; Rodrigues, Maria João; Pereira, Hugo; Gaspar, Helena; Malcata, F. Xavier; Barreira, Luísa; Varela, JoãoTisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extracts exhibited in vitro cytotoxic activity against human hepatocarcinoma (HepG2) cells. However, so far, the compound(s) accountable for the observed bioactivity have not been identified. Therefore, the aim of this study was to isolate and identify the chemical component(s) responsible for the bioactivity observed. Bioassay-guided fractionation through a combination of silica-gel column chromatography, followed by preparative thin layer chromatography (PTLC), led to the isolation of two diastereomers of a monoterpenoid lactone, namely, loliolide (1) and epi-loliolide (2), isolated for the first time in this species. The structural elucidation of both compounds was carried out by GC-MS and 1D (1 H and13 C APT) and 2D (COSY, HMBC, HSQC-ed, and NOESY) NMR analysis. Both compounds significantly reduced the viability of HepG2 cells and were considerably less toxic towards a non-tumoral murine stromal (S17) cell line, although epi-loliolide was found to be more active than loliolide. © 2020 by the authors.
- Atividade física, exercício físico e fitness em Portugal: passado, presente e futuroPublication . Monteiro, Diogo; Chaves, César; Bastos, Vasco; Pereira, Hugo; Teixeira, Diogo; Rodrigues, FilipeThis article sought, analytically and narratively, to analyze the trends in physical activity and exercise practice in Portugal over the past two decades. Additionally, the context of fitness in the national territory was examined, based on records released over the past years. Specifically, physical exercise was analyzed based on the fitness context, where interest was focused on the economic response to attrition rates and the weekly frequency of members. The current work is structured into several parts, namely: i) physical activity practiced in Portugal; ii) the panorama of physical exercise and fitness in Portugal; and iii) the economic analysis of the fitness sector in Portugal; iv) levels of physical exercise, and v) reasons for non-practice and abandonment of physical exercise. Finally, we will present a discussion highlighting practical implications. In conclusion, this study highlights the difficulty of the fitness sector in reversing inactivity trends. There is a need to review the methods used to assess physical activity and exercise. Additionally, it underscores the importance of understanding the reasons for dropout in fitness centers and implementing reforms to improve customer experience and reduce attrition rates.
- History of Fitness in Portugal: A Brief Review of the Influences that Shaped the Current National PanoramaPublication . Teixeira, Diogo; Cerca, Luís; Bastos, Vasco; Rodrigues, Filipe; Pereira, HugoFitness in Portugal results from a set of historical intersections marked by various influences and personalities. To a large extent, knowledge of this path is essential to understanding the current reality and some of the problems the area faces. The present opinion intends to situate and summarise the leading influences of national and international events and some time frames that led to the development of the intervention area commonly called fitness. For this purpose, the work is structured in three parts: i) the beginnings of fitness, where some of the international influences that were at the origin of this area will be presented; ii) some of the main influences and factors that allowed the growth of fitness in Portugal; and iii) the effect of the historical trajectory of fitness in the current context and future considerations about the area. Given the scarcity of records on the subject, the present work, as an opinion article, will also be a self-report of the authors, who, having experienced different moments of this history, intend to leave a contribution to this record and for future reflections of its impact on the development of the area, professionals, and respective practices.
- Treino personalizado: recomendações para a elevação da qualidade do serviço prestadoPublication . Teixeira, Diogo Santos; Pereira, Hugo; Sousa, Ana; Chaves, César; Ruivo, Rodrigo; Asseisseira, Pedro; Dias, Amândio; Monteiro, Diogo; Cid, LuisA intervenção individualizada estruturada através do treino personalizado é uma ferramenta importante para os profissionais de exercício físico e um serviço de potencial relevo na saúde e bem-estar dos praticantes. O treino personalizado é uma prática desenvolvida nacionalmente por milhares de profissionais e relativamente comum em vários contextos, onde os ginásios e health clubs se destacam em particular. Considerando a reduzida produção nacional de literatura neste tópico, e assumindo a importância e relevância da emissão de recomendações atualizadas e que derivem de limitações concretas observadas em contexto de prática, este artigo apresenta como objetivo principal emitir orientações e recomendações operacionais que permitam uma aferição e regulação do trabalho implementado neste contexto, visando uma reflexão crítica e melhoria do serviço prestado. As recomendações emitidas também procuram, em acréscimo ao apoio associado ao desempenho da função profissional, ajudar na clarificação do que deverá constituir e delimitar a prestação deste serviço perante a visão de um praticante, apontando um caminho que vise uma uniformização de comportamentos e ações ética e profissionalmente recomendáveis, que possam e devam ser assumidas na prestação do treino personalizado, e permitam criar uma imagem social mais clara, objetiva e delimitada deste serviço individualizado.