Browsing by Author "Bártolo, Paulo Jorge"
Now showing 1 - 6 of 6
Results Per Page
Sort Options
- Bio-Materials and Prototyping Applications in MedicinePublication . Bártolo, Paulo Jorge; Bidanda, Bopaya; Bártolo, Paulo Jorge; Bidanda, BopayaThis second edition maintains a focus on integrated biomaterials, computer-aided design, and physical prototyping techniques as examples of the materials and applications that are found in medical environments. All original chapters, written by renowned experts in the field, have been updated along with the addition of four new chapters on: • Smart insoles • Medical applications of additive manufacturing • Additive manufacturing in craniofacial applications • Additive manufacturing in hearing aids This wide-ranging treatise on biomaterials and prototyping applications in medicine also focuses on solid freeform fabrication, rapid prototyping, layered manufacturing, and computer-aided design in the development of prosthetic devices. This book is a must-have for bioengineers seeking a comprehensive overview of this important subject and examples of medical applications, as well as researchers and academics in the same field.
- Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementationPublication . Moura, Carla; Silva, João Carlos; Faria, Sofia; Fernandes, Paulo Rui; Silva, Cláudia Lobato da; Cabral, Joaquim Manuel Sampaio; Linhardt, Robert; Bártolo, Paulo Jorge; Ferreira, Frederico CasteloCartilage defects resultant from trauma or degenerative diseases (e.g., osteoarthritis) can potentially be repaired using tissue engineering (TE) strategies combining progenitor cells, biomaterial scaffolds and bio physical/chemical cues. This work examines promoting chondrogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BMMSCs) by combining the effects of modified poly (ε-caprolactone) (PCL) scaffolds hydrophilicity and chondroitin sulfate (CS) supplementation in a hypoxic 5% oxygen atmosphere. 3D extruded PCL scaffolds, characterized by mCT, featured a 21 mmL1 surface area to volume ratio, 390 mm pore size and approximately 100% pore interconnectivity. Scaffold immersion in sodium hydroxide solutions for different periods of time had major effects in scaffold surface morphology, wettability and mechanical properties, but without improvements on cell adhesion. In-situ chondrogenic differentiation of BM-MSC seeded in 3D-extruded PCL scaffolds resulted in higher cell populations and ECM deposition along all scaffold structure, when chondrogenesis was preceded by an expansion phase. Additionally, CS supplementation during BM-MSC expansion was crucial to enhance aggrecan gene expression, known as a hallmark of chondrogenesis. Overall, this study presents an approach to tailor the wettability and mechanical properties of PCL scaffolds and supports the use of CSsupplementation as a biochemical cue in integrated TE strategies for cartilage regeneration.
- Comparison of Three-dimensional Extruded Poly (ɛ-Caprolactone) and Polylactic acid Scaffolds with Pore size VariationPublication . Monteiro de Moura, Carla Sofia; Ferreira, Frederico Castelo; Bártolo, Paulo JorgeAdditive manufacturing (AM) has become a prominent approach among the scientific community for the production of three-dimensional (3D) matrices able to support tissue engineering approaches, promoting cell adhesion, proliferation and organization aiming to repair different tissues, such as bone or cartilage. In this study we used an extrusion-based technique for the production of poly (ɛ-caprolactone) (PCL) and polylactic acid (PLA) scaffolds and performed a side-by-side scaffold characteristics comparison. Using this technique we were able to create fully 3D interconnected porous scaffolds with pore size variations ranging from 190 μm to 390 μm with both materials. These scaffolds were assessed for stiffness, wettability and cell adhesion using mesenchymal stem/stromal cells (MSC). Comparisons between these two materials were made. The compressive modulus obtained is on the same order of magnitude for both materials. However, PCL presents a statistically significant higher compressive modulus. Results confirmed that PCL is a more hydrophobic material, so it presents a lower wettability when compared to PLA. Interestingly cell adhesion is similar for PLA and PCL, therefore selection between these two materials for the use of this versatile platform can be defined according with biodegradability aimed.
- A new design of an electrospinning apparatus for tissue engineering applicationsPublication . Dias, Juliana R.; Santos, Cyril dos; Horta, João; Granja, Pedro Lopes; Bártolo, Paulo JorgeThe electrospinning technique is being widely explored in the biomedical field due to its simplicity to produce meshes and its capacity to mimic the micro-nanostructure of the natural extracellular matrix. For skin tissue engineering applications, wound dressings made from electrospun nanofibers present several advantages compared to conventional dressings, such as the promotion of the hemostasis phase, wound exudate absorption, semi-permeability, easy conformability to the wound, functional ability and no scar induction. Despite being a relatively simple technique, electrospinning is strongly influenced by polymer solution characteristics, processing parameters and environmental conditions, which strongly determine the production of fibers and their morphology. However, most electrospinning systems are wrongly designed, presenting a large number of conductive components that compromises the stability of the spinning process. This paper presents a new design of an electrospinning system solving the abovementioned limitations. The system was assessed through the production of polycaprolactone (PCL) and gelatin nanofibers. Different solvents and processing parameters were considered. Results show that the proposed electrospinning system is suitable to produce reproducible and homogeneous electrospun fibers for tissue engineering applications.
- PrefacePublication . Fernandes, Paulo Rui; Bártolo, Paulo JorgeThis book is a contribution for Tissue Engineering seen as multidisciplinary field involving scientists from different backgrounds like medicine, chemistry, material science, engineering and biology with a focus on the development of mathematical methods that are quite relevant to understand cell biology and human tissues as well to model, design and fabricate optimized and smart scaffolds.
- Virtual Prototyping & Bio Manufacturing in Medical ApplicationsPublication . Bidanda, Bopaya; Bártolo, Paulo Jorge; Bidanda, Bopaya; Bártolo, Paulo JorgeThis new edition focuses on modeling and manufacturing in the field of prototyping and bio manufacturing. The principles utilized draw heavily from more traditional engineering fields including mechanical, industrial, civil (structures), electrical, and bio engineering. Written for engineers and academics seeking a comprehensive overview of virtual prototyping and rapid prototyping, this book discusses in detail applications related to surgery, medical imaging, tissue engineering, bone replacement, and more. Seven new chapters address: • Two-photon polymerization for tissue engineering • Molding in medicine • Organ printing • Skin printing • Nerve regeneration and spinal injury repair • Cartilage regeneration • And Structural analysis of the human shoulder.