Evaluating the in vitro potential of natural extracts to protect lipids from oxidative damage

Félix, Rafael; Valentão, Patrícia; Andrade, Paula B.; Félix, Carina; Novais, Sara C.; Lemos, Marco F.L.

http://hdl.handle.net/10400.8/5088

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
Félix et al_2020_antioxidant.pdf		1.26 MB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

Lipid peroxidation is a chemical reaction known to have negative impacts on living organisms’ health and on consumer products’ quality and safety. Therefore, it has been the subject of extensive scientific research concerning the possibilities to reduce it, both in vivo and in nonliving organic matrices. It can be started by a variety of oxidants, by both ROS-dependent and -independent pathways, all of them reviewed in this document. Another feature of this reaction is the capacity of lipid peroxyl radicals to react with the non-oxidized lipids, propagating the reaction even in the absence of an external trigger. Due to these specificities of lipid peroxidation, regular antioxidant strategies—although being helpful in controlling oxidative triggers—are not tailored to tackle this challenge. Thus, more suited antioxidant compounds or technologies are required and sought after by researchers, either in the fields of medicine and physiology, or in product development and biotechnology. Despite the existence of several laboratory procedures associated with the study of lipid peroxidation, a methodology to perform bioprospecting of natural products to prevent lipid peroxidation (a Lipid Peroxidation Inhibitory Potential assay, LPIP) is not yet well established. In this review, a critical look into the possibility of testing the capacity of natural products to inhibit lipid peroxidation is presented. In vitro systems used to peroxidize a lipid sample are also reviewed on the basis of lipid substrate origin, and, for each of them, procedural insights, oxidation initiation strategies, and lipid peroxidation extent monitoring are discussed.

Description

This study was supported by UIDB/50006/2020 with funding from FCT/MCTES through national funds, by the FCT Strategic Project UID/MAR/04292/2019 granted to MARE, and by the grants awarded to Rafael Félix (SFRH/ BD/139763/2018). The authors also wish to acknowledge the support of the European Union through EASME Blue Labs project AMALIA, Algae-to-MArket Lab IdeAs (EASME/EMFF/2016/1.2.1.4/03/SI2.750419), project VALORMAR (Mobilizing R&TD Programs, Portugal 2020) co-funded by COMPETE (POCI-01-0247-FEDER-024517), the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund, SAICTPAC/0019/2015—LISBOA-01-0145-FEDER-016405 Oncologia de Precisão: Terapias e Tecnologias Inovadoras (POINT4PAC), and project “Valorização dos subprodutos do processo biotecnológico de produção de esqualeno e DHA pela microalga Aurantiochytrium sp.” (AlgaValue) (ref. 17680), ANI—Agência Nacional de Inovação, S.A., FEDER—Fundo Europeu de Desenvolvimento Regional.

Keywords

Lipid peroxidation LPIP Natural products Bioactive compounds In vitro

URI

http://hdl.handle.net/10400.8/5088

Citation

Félix, R.; Valentão, P.; Andrade, P.B.; Félix, C.; Novais, S.C.; Lemos, M.F.L. Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage. Antioxidants 2020, 9, 231.