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Publication
Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta)
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Advisor(s)
Abstract(s)
Brachionus plicatilis is a cosmopolitan rotifer used as a model organism in several research areas and as live food in aquaculture. Being a species complex, responses to stressors vary even among strains of the same species and, thus, the responses of one species are not representative of the whole complex. This study aimed to address the effects of extreme salinity ranges, and different concentrations of hydrogen peroxide, copper, cadmium, and chloramphenicol, in two strains of B. koreanus (MRS10 and IBA3) from B. plicatilis species complex, by assessing effects on their survival and swimming capacity. Neonates (0–4 h old) were exposed to the stressors in 48 well-microplates, for 24 and 6 h, to evaluate lethal and behavioural effects, respectively. Tested conditions of chloramphenicol did not show any effects on rotifers. The behavioural endpoint showed to be particularly sensitive to assess the effects of high salinity, hydrogen peroxide, and copper sulfate, as swimming capacity impairment was observed for both strains in the lowest concentrations used in lethal tests. Overall, results showed that IBA3 was more tolerant to the majority of stressors, comparing to MRS10, which may be due to differences in physiological characteristics, highlighting the importance of performing multiclonal experiments. Also, swimming capacity inhibition proved to be a good alternative to the classical lethality tests, being sensitive to lower concentrations and with shorter exposure periods.
Description
Open access funding provided by FCT|FCCN (b-on). This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project granted to MARE – Marine and Environmental Sciences Centre (UIDB/04292/2020 and UIDP/04292/2020) and the project granted to the Associated Laboratory ARNET
(LA/P/0069/2020). Luana Granada also wish to acknowledge the financial support given by FCT (SFRH/BD/102036/2014). Sara Novais is funded by national funds through FCT, in the scope of the framework
contract foreseen in the numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July.
Keywords
Aquaculture Behavior Brachionus koreanus Metals Monogonont rotifer Swimming capacity
Citation
Granada L., Lemos M.F.L., Bossier P., Novais S.C. AUTHOR FULL NAMES: Granada, Luana (56513933800); Lemos, Marco F. L. (7006042884); Bossier, Peter (6603773863); Novais, Sara C. (23025463300) 56513933800; 7006042884; 6603773863; 23025463300 Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta) (2023) Environmental Science and Pollution Research, Cited 0 times. DOI: 10.1007/s11356-023-26190-3
Publisher
Springer Science and Business Media