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Publication
Evaluation of multivariate biomarker indexes application in ecotoxicity tests with marine diatoms exposed to emerging contaminants
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Advisor(s)
Abstract(s)
Worldwide anthropogenic activities result in the production and release of potentially damaging toxic pollutants into ecosystems, thereby jeopardizing their health and continuity. Research studies and biomonitoring programs attend to this emerging problematic by applying and developing statistically relevant indexes that integrate complex biomarker response data to provide a holistic approach, reflecting toxically induced alterations at the organism or population level.
Ultimately, indexes allow simple result communications, enhancing policy makers understanding,
and contributing to better resource and environmental managing policies. In this study three indexes,
the integrated biomarker response index (IBR), the bioeffects assessment index (BAI) and principal
components analysis (PCA), were evaluated for their sensitivity in revealing toxically induced stress
patterns in cells of the diatom Phaeodactylum tricornutum under contaminant exposure. The set of
biomarkers selected for index construction comprised the anti-oxidant enzymes APX, CAT and SOD,
and the lipid peroxidation marker TBARS. Several significant correlations with the applied concentration
gradients were noticed for all indexes, although IBR excelled for its reliability in delivering statistically significant dose-response patterns for four out of the five tested compounds.
Description
The authors would like to thank Fundação para a Ciência e a Tecnologia (FCT) for
funding the research via project grants PTDC/MAR-EST/3048/2014 (BIOPHARMA), PTDC/CTAAMB/30056/2017 (OPTOX), UIDB/04292/2020 and UID/MULTI/04046/2019. Work was also
funded by the Integrated Programme of SR&TD SmartBioR (reference Centro-01-0145-FEDER000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European
Regional Development Fund. B. Duarte and V. F. Fonseca were supported by researcher contracts
(CEECIND/00511/2017 and DL57/2016/CP1479/CT0024).
Keywords
Antioxidant enzymes Pharmaceutical residues Pesticides Detergents Integrative indexes
Citation
Pires, V.L.; Novais, S.C.; Lemos, M.F.L.; Fonseca, V.F.; Duarte, B. Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants. Appl. Sci. 2021, 11, 3878. https://doi.org/10.3390/ app11093878
Publisher
MDPI