Argan oil as a rich source of linoleic fatty acid for dietetic structured lipids production

Simões, Tiago; Ferreira, Jessica; Lemos, Marco F.L.; Augusto, Ana; Félix, Rafael; Silva, Susana F.J.; Ferreira-Dias, Suzana; Tecelão, Carla

http://hdl.handle.net/10400.8/7577

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Authors

Ferreira-Dias, Suzana

Tecelão, Carla

Abstract(s)

Argan oil is rich in long-chain unsaturated fatty acids (FA), mostly oleic and linoleic, and natural antioxidants. This study addresses the production of low-calorie structured lipids by acidolysis reaction, in a solvent-free system, between caprylic (C8:0; system I) or capric (C10:0; system II) acids and argan oil, used as triacylglycerol (TAG) source. Three commercial immobilized lipases were tested: Novozym® 435, Lipozyme® TL IM, and Lipozyme® RM IM. Higher incorporation degree (ID) was achieved when C10:0 was used as acyl donor, for all the lipases tested. Lipozyme® RM IM yielded the highest ID for both systems (28.9 +- 0.05 mol.% C10:0, and 11.4 +- 2.2 mol.% C8:0), being the only catalyst able to incorporate C8:0 under the reaction conditions for biocatalyst screening (molar ratio 2:1 FA/TAG and 55ºC). The optimal conditions for Lipozyme® RM IM in system II were found by response surface methodology (66ºC; molar ratio FA/TAG of 4:1), enabling to reach an ID of 40.9 mol.% of C10:0. Operational stability of Lipozyme® RM IM in system II was also evaluated under optimal conditions, after eight consecutive 24 h-batches, with biocatalyst rehydration between cycles. The biocatalyst presented a half-life time of 103 h.

Description

Funding: This study had the support of Fundação para a Ciência e a Tecnologia (FCT), Portugal, to the research units of MARE (UID/MAR/04292/2020) and LEAF-Linking Landscape, Environment, Agriculture and Food Research Centre (UIDB/04129/2020), and of the grants awarded to Tiago Simões (SFRH/BD/98266/2013), Ana Augusto (SFRH/BD/131465/2017) and Rafael Félix (SFRH/ BD/139763/2018). The authors also acknowledge the project ValorMar (24517, 10/SI/2016- Mobilizing R&TD Programs, Portugal 2020), co-funded by the European Regional Development Fund (ERDF) and by the European Social Fund (ESF). The work was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) co-funded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund.

Keywords

Argania spinosa oil Capric acid Caprylic acid Commercial immobilized lipases Low-calorie structured lipids

URI

http://hdl.handle.net/10400.8/7577

Citation

Simões, T.; Ferreira, J.; Lemos, M.F.L.; Augusto, A.; Félix, R.; Silva, S.F.J.; Ferreira-Dias, S.; Tecelão, C. Argan Oil as a Rich Source of Linoleic Fatty Acid for Dietetic Structured Lipids Production. Life 2021, 11, 1114. https://doi.org/10.3390/life11111114