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Physicochemical and nutritional characterization of the leaves, flowers and fruits of Chorão da praia (Carpobrotus edulis) from Portuguese west shores

2020Conference object Open access
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dc.contributor.authorNeves, Marta
dc.contributor.authorAntunes, Madalena
dc.contributor.authorAzevedo, Zélia M.
dc.contributor.authorFreitas, Victor
dc.contributor.authorRocha, João M.
dc.contributor.authorTecelão, Carla
dc.date.accessioned2021-02-24T15:06:06Z
dc.date.available2021-02-24T15:06:06Z
dc.date.issued2020
dc.descriptionThis study had the support of Fundação para a Ciência e a Tecnologia (FCT), Portugal, to the research unit of MARE (UID/MAR /04292/2019). The authors M.N., M.A., W.F. and C.T. also wish to acknowledge the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund and the Project Ocean2Oils “Integrated approach for seaweeds application as sustainable source of functional compounds for edible oils stabilization and food processing”, co-funded by Fundo Azul program through the Direção-Geral de Política do Mar. This work is based upon the work from COST Action 18101 SOURDOMICS - Sourdough biotechnology network towards novel, healthier and sustainable food and bioprocesses, where the author J.M.R is the Chair and Grant Holder Scientific Representative. The authors J.M.R., V.F. and Z.M.A. acknowledge the Institute of Sciences, Technologies and Agribusiness (ICETA), as well as the Green Chemistry Laboratory (LAQV), Chemistry and Technology Network (REQUIMTE), Department of Chemistry and Biochemistry (DQB), Faculty of Sciences of University of Porto (FCUP), and the Food Polyphenol Lab, Research group of Applied Organic Chemistry (QUINOA).
dc.description.abstractLeaves, flowers and fruits from the edible halophyte Chorão da praia (Carpobrotus edulis), harvested in Portuguese west shores, were characterized regarding their proximate composition, phenolic compounds and antioxidant activity. Flowers and leaves had similar protein content (5.42±0.15 and 5.22 ±0.13 % DW, respectively), being higher than fruits (4.67±0.22 %). No significant differences were observed in the lipid fraction, ranging from 1.70 ± 0.12 in flowers to 1.90 ± 0.16 % DW in leaves. Fruits presented the highest value of total carbohydrates (71.5±1.2 % DW), whereas ash was more abundant in leaves (32.00 ± 0.35 % DW) that also showed the highest chlorophylls content, comprising 52.0±7.2 μg/g chl a and 26.2±8.2 μg/g chl b. Similar levels of total carotenoids were observed in leaves and flowers (5.7±1.6 and 4.6±1.4 μg/g DW, respectively), being significantly higher than fruits (2.8±0.2 μg/g DW). Aqueous-ethanolic (1:1, v/v) extract of flowers exhibited the highest total phenolic content (299±8 μg/mg of gallic acid equivalents), by the Folin–Ciocalteu method, as well as the strongest antioxidant activity (3.25±0.21 and 2.38±0.07 μmol/mg of trolox equivalents by the DPPH and FRAP methods, respectively), followed by leaves (1.68±0.16 and 1.36±0.14 μmol/mg) and fruits (0.58±0.13 and 0.71±0.03 μmol/mg). Bulk polyphenol extracts were further purified through Solid-Phase Extraction (SPE), using C18 reversed-phase sorbent Oasis® Prime HLB Extraction cartridges. Polyphenols in purified extracts were initially analyzed by RP-HPLC-DAD and a separation chromatographic method was developed and optimized. The optimal elution program was further used for the separation and identification of polyphenols via RP-HPLC-ESI-MS/MS. Mass spectra unfolded the complete or partial separation of molecular species of polyphenols. Moreover, mass and tandem mass spectra revealed that the most abundant species in all extracts were glycosylated and/or acetylated O-Methyl flavonols. It was observed compounds mainly derived from syringetin and laricitrin. Each of these two aglycones revealed two major compounds: two [MH]- ions with m/z 653 (syringetine derivatives) and two [M-H]- ions with m/z 639 (laricitrin derivatives). In both cases, the MS2 data of the [M-H]- ions showed high intensity fragment ions with m/z 345 and 331, respectively, corresponding to the loose of a glucosyl-alfa-araninofuranoside or an acetylated glucoside [M-308]. Moreover, the MS3 data of the two most intense fragment resulted in successive loss of methyl groups with the first one presenting the highest intensity. Regarding the profile in polyphenols, when comparing leaves, flowers and fruits, one concluded that there are clearly differences in the relative amount of these polyphenols being flowers richer in the syringetin derivatives.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNeves, M., Antunes, M., Fernandes, W., Azevedo, Z.M., Freitas, V., Rocha, J.M., & Tecelão, C. (2020, Nov. 5-6). Physicochemical and nutritional characterization of the leaves, flowers and fruits of Chorão da praia (Carpobrotus edulis) from Portuguese west shores [Abstract]. IberPhenol International Conference, Coimbra, Portugalpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.8/5390
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.relationMARE - Marine and Environmental Sciences Centre
dc.subjectPhenolpt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectCarpobrotus edulispt_PT
dc.titlePhysicochemical and nutritional characterization of the leaves, flowers and fruits of Chorão da praia (Carpobrotus edulis) from Portuguese west shorespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMARE - Marine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2019/PT
oaire.citation.startPage46pt_PT
oaire.citation.titleiberPhenol International Conference: Advances in the role of phenols in health effects and other uses, Coimbra, Portugal, 5-6 November 2020pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamede Melo Lopes Neves
person.familyNameAntunes
person.familyNameTecelão
person.givenNameMarta Maria
person.givenNameMadalena
person.givenNameCarla
person.identifier1685584
person.identifier.ciencia-idEF15-1119-B28F
person.identifier.ciencia-idAB15-9BB4-375F
person.identifier.ciencia-id9B1D-4AC0-1B03
person.identifier.orcid0000-0003-1553-4745
person.identifier.orcid0000-0003-2451-1406
person.identifier.orcid0000-0003-2423-0495
person.identifier.scopus-author-id7801371939
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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