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Effects of Anaesthesia on Insulin Sensitivity and Metabolic Parameters in Wistar Rats

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datacite.subject.fosCiências Médicas::Medicina Clínica
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg17:Parcerias para a Implementação dos Objetivos
dc.contributor.authorGuarino, Maria Pedro
dc.contributor.authorSantos, Ana Isabel
dc.contributor.authorMota-Carmo, Miguel
dc.contributor.authorCosta, Pedro Freire
dc.date.accessioned2026-03-25T15:09:01Z
dc.date.available2026-03-25T15:09:01Z
dc.date.issued2013
dc.description.abstractWe compared the effects of two different anaesthetics, sodium pentobarbital (65 mg/kg) and ketamine (30 mg/kg) plus xylazine (4 mg/kg) (KX) on insulin sensitivity, fasting glycaemia, insulinaemia and free fatty acids (FFA). Four groups of Wistar rats were used: KX group (n=6), pentobarbital group (n=6), high-sucrose diet group (n=6) and the conscious group (n=6). The insulin tolerance test (ITT) was used to measure insulin sensitivity, and metabolic biomarkers were determined using commercial kits. Pentobarbital did not alter plasma insulin, glucose, FFA or the ITT results compared to conscious animals. In contrast, KX anaesthesia induced hyperglycaemia, increased serum FFA and altered the ITT results compared to the conscious animal group. Moreover, under pentobarbital anaesthesia, the ITT proved to be a suitable method to detect insulin resistance in an animal model of diet-induced insulin resistance. We concluded that sodium pentobarbital anaesthesia should be used in metabolic studies since it does not interfere with plasma glucose, insulin, F FA or insulin sensitivity quantification in Wistar rats.eng
dc.description.sponsorshipWe wish to acknowledge Gracinda Menezes and Mariana Lavajo for technical support and Abbot Diabetes Care, Portugal for the glucometers and test strips
dc.identifier.citationGuarino, M. P., Santos, A. I., Mota-Carmo, M., & Costa, P. F. (2013). Effects of anaesthesia on insulin sensitivity and metabolic parameters in Wistar rats. in vivo, 27(1), 127-132.
dc.identifier.issn0258-851X
dc.identifier.urihttp://hdl.handle.net/10400.8/15993
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInternational Institute of Anticancer Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAnaesthesia
dc.subjectInsulin sensitivity
dc.subjectKetamine/xylazine
dc.subjectPentobarbital
dc.subjectSucrose-fed rat
dc.subjectWistar rats
dc.titleEffects of Anaesthesia on Insulin Sensitivity and Metabolic Parameters in Wistar Ratseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage132
oaire.citation.issue1
oaire.citation.startPage127
oaire.citation.titleIn Vivo
oaire.citation.volume27
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGuarino
person.givenNameMaria Pedro
person.identifier.ciencia-idF21A-BD01-2D52
person.identifier.orcid0000-0001-6079-1105
person.identifier.ridB-5594-2015
person.identifier.scopus-author-id56348477000
relation.isAuthorOfPublicationf163d4df-1e45-4278-affa-5994f206becf
relation.isAuthorOfPublication.latestForDiscoveryf163d4df-1e45-4278-affa-5994f206becf

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