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Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly: In Vitro and In Vivo Analysis

2014-07-10Artigo de investigação Acesso aberto
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datacite.subject.fosCiências Médicas::Medicina Básica
datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorPereira, T.
dc.contributor.authorGärtner, A.
dc.contributor.authorAmorim, I.
dc.contributor.authorAlmeida, A.
dc.contributor.authorCaseiro, A.R.
dc.contributor.authorArmada-da-Silva, Paulo A. S.
dc.contributor.authorAmado, Sandra
dc.contributor.authorFregnan, Federica
dc.contributor.authorVarejão, A. S. P.
dc.contributor.authorSantos, J. D.
dc.contributor.authorBártolo, P. J.
dc.contributor.authorGeuna, S.
dc.contributor.authorLuís, A. L.
dc.contributor.authorMaurício, A. C.
dc.date.accessioned2026-05-08T11:18:14Z
dc.date.available2026-05-08T11:18:14Z
dc.date.issued2014-07-10
dc.descriptionArticle number - 302659.
dc.descriptionAcknowledgments The authors would like to gratefully acknowledge the valuable support of José Manuel Correia Costa, from Laboratório de Parasitologia, Instituto Nacional de Saúde Dr. Ricardo Jorge (INSRJ), Porto, Portugal. They would also like to acknowledge the support from Liliana Matos from the Human Genetics Lab of the same Institute for the technical support in the nucleic acid preparation. The authors would also like to gratefully acknowledge Simone Bompasso for the technical assistance for the histological processing of tissues.
dc.description.abstractIn peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-<jats:italic>ε</jats:italic>-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results<jats:italic>in vitro</jats:italic>showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed.<jats:italic>In vivo</jats:italic>testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery.eng
dc.description.sponsorshipThe authors would like to acknowledge the financial support from the program COMPETE, Programa Operacional Factores de Competitividade, project Pest-OE/AGR/UI0211/2011, from projects QREN I&DT Cluster in Development of Products for Regenerative Medicine and Cell Therapies, Projects Biomat & Cell QREN 2008/1372. This project was also cofinanced by the European Community FEDER fund through ON2—O Novo Norte—North Portugal Regional Operational Program 2007–2013, by project “Hybrid Nanostructured Hydrogels: Bone regeneration using Multifunctional injectable Hydrogels, Rebone,” ENMED/0002/2010 from FCT, Ministério da Educação e da Ciência, and Program Project Euronanomed, reference EraNet-EuroNanoMed JTC2010 and from FCT project PTDC/CVT/103081/2008. The coauthor A Almeida is supported by a Post-Doc grant from FCT under the reference SFRH/BPD/79539/2011.
dc.identifier.citationPereira, T., Gärtner, A., Amorim, I., Almeida, A., Caseiro, A. R., Armada-da-Silva, P. A. S., Amado, S., Fregnan, F., Varejão, A. S. P., Santos, J. D., Bartolo, P. J., Geuna, S., Luís, A. L., & Maurício, A. C. (2014). Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly: In Vitro and In Vivo Analysis. BioMed Research International, 2014, Article ID 302659. https://doi.org/10.1155/2014/302659
dc.identifier.doi10.1155/2014/302659
dc.identifier.issn2314-6133
dc.identifier.issn2314-6141
dc.identifier.urihttp://hdl.handle.net/10400.8/16257
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationStrategic Project - UI 211 - 2011-2012
dc.relationHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
dc.relationNew approaches for inactivation, treatment and immunoprophylaxy of cryptosporidiosis
dc.relationANTIBODIES AND RECOMBINANT PROTEIN DEVELOPMENT: TOOLS FOR DIAGNOSIS AND IMMUNOPROPHYLAXIS OF INFECTIOUS DISEASES
dc.relation.hasversionhttps://onlinelibrary.wiley.com/doi/10.1155/2014/302659
dc.relation.ispartofBioMed Research International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectnerve regeneration
dc.subjectneurotmesis
dc.subjectrat model
dc.subjectpoly(DL-lactide-ε-caprolactone)
dc.subjectmesenchymal stem cells
dc.subjectWharton’s jelly
dc.titlePromoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly: In Vitro and In Vivo Analysiseng
dc.typeresearch article
dspace.entity.typePublication
oaire.awardNumberPEst-OE/AGR/UI0211/2011
oaire.awardNumberENMED/0002/2010
oaire.awardNumberPTDC/CVT/103081/2008
oaire.awardNumberSFRH/BPD/79539/2011
oaire.awardTitleStrategic Project - UI 211 - 2011-2012
oaire.awardTitleHybrid Nanostuctured Hydrogels: Bone regeneration using multifunctional injectable hydrogels
oaire.awardTitleNew approaches for inactivation, treatment and immunoprophylaxy of cryptosporidiosis
oaire.awardTitleANTIBODIES AND RECOMBINANT PROTEIN DEVELOPMENT: TOOLS FOR DIAGNOSIS AND IMMUNOPROPHYLAXIS OF INFECTIOUS DISEASES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0211%2F2011/PT
oaire.awardURIhttp://hdl.handle.net/10400.8/16236
oaire.awardURIhttp://hdl.handle.net/10400.8/14408
oaire.awardURIhttp://hdl.handle.net/10400.8/16256
oaire.citation.endPage17
oaire.citation.startPage1
oaire.citation.titleBioMed Research International
oaire.citation.volume2014
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamEuroNanoMed - Second Joint Call 2010
oaire.fundingStreamConcurso para Projectos de I&D em todos os Domínios Científicos - 2008
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCaseiro Santos
person.familyNameAmado
person.familyNameBartolo
person.givenNameAna Rita
person.givenNameSandra
person.givenNamePaulo
person.identifier804352
person.identifier203086
person.identifier.ciencia-idD617-149A-8102
person.identifier.ciencia-id5810-9BF9-4522
person.identifier.orcid0000-0002-1143-2461
person.identifier.orcid0000-0001-8356-4810
person.identifier.orcid0000-0003-3683-726X
person.identifier.ridO-6988-2016
person.identifier.ridF-2421-2013
person.identifier.scopus-author-id16038616600
person.identifier.scopus-author-id6603353041
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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