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Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly: In Vitro and In Vivo Analysis
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Orientador(es)
Resumo(s)
In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-<jats:italic>ε</jats:italic>-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results<jats:italic>in vitro</jats:italic>showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed.<jats:italic>In vivo</jats:italic>testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery.
Descrição
Article number - 302659.
Acknowledgments The authors would like to gratefully acknowledge the valuable support of José Manuel Correia Costa, from Laboratório de Parasitologia, Instituto Nacional de Saúde Dr. Ricardo Jorge (INSRJ), Porto, Portugal. They would also like to acknowledge the support from Liliana Matos from the Human Genetics Lab of the same Institute for the technical support in the nucleic acid preparation. The authors would also like to gratefully acknowledge Simone Bompasso for the technical assistance for the histological processing of tissues.
Acknowledgments The authors would like to gratefully acknowledge the valuable support of José Manuel Correia Costa, from Laboratório de Parasitologia, Instituto Nacional de Saúde Dr. Ricardo Jorge (INSRJ), Porto, Portugal. They would also like to acknowledge the support from Liliana Matos from the Human Genetics Lab of the same Institute for the technical support in the nucleic acid preparation. The authors would also like to gratefully acknowledge Simone Bompasso for the technical assistance for the histological processing of tissues.
Palavras-chave
nerve regeneration neurotmesis rat model poly(DL-lactide-ε-caprolactone) mesenchymal stem cells Wharton’s jelly
Contexto Educativo
Citação
Pereira, T., Gärtner, A., Amorim, I., Almeida, A., Caseiro, A. R., Armada-da-Silva, P. A. S., Amado, S., Fregnan, F., Varejão, A. S. P., Santos, J. D., Bartolo, P. J., Geuna, S., Luís, A. L., & Maurício, A. C. (2014). Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly: In Vitro and In Vivo Analysis. BioMed Research International, 2014, Article ID 302659. https://doi.org/10.1155/2014/302659
Editora
Wiley