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Publication
Chironomus riparius proteome responses to spinosad exposure
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Advisor(s)
Abstract(s)
The potential of proteome responses as early-warning indicators of insecticide exposure was evaluated using the non-biting midge Chironomus riparius (Meigen) as the model organism. Chironomus riparius larvae were exposed to environmentally relevant concentrations of the neurotoxic pesticide spinosad to uncover molecular events that may provide insights on the long-term individual and population level consequences. The iTRAQ labeling method was performed to quantify protein abundance changes between exposed and non-exposed organisms. Data analysis revealed a general dose-dependent decrease in the abundance of globin proteins as a result of spinosad exposure. Additionally, the downregulation of actin and a larval cuticle protein was also observed after spinosad exposure, which may be related to previously determined C. riparius life-history traits impairment and biochemical responses. Present results suggest that protein profile changes can be used as early warning biomarkers of pesticide exposure and may provide a better mechanistic interpretation of the toxic response of organisms, aiding in the assessment of the ecological effects of environmental contamination. This work also contributes to the understanding of the sublethal effects of insecticides in invertebrates and their molecular targets.
Description
Funding: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UIDB/04292/2020, CESAM: UIDP/50017/2020+UIDB/50017/2020, FCT/MCTES through national funds, the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020, and project PROTEOME (PTDC/AAG-MAA/1302/2014) co-financed by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Program of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, and IF/01420/2015, respectively).
Keywords
Aquatic insects Ecotoxicoproteomics Hemoglobin iTRAQ Neurotoxic insecticides
Citation
Monteiro HR, Pestana JLT, Soares AMVM, Devreese B, Lemos MFL. Chironomus riparius Proteome Responses to Spinosad Exposure. Toxics. 2020 Dec 11;8(4):117. doi: 10.3390/toxics8040117. PMID: 33322338; PMCID: PMC7768432.
Publisher
MDPI