Repository logo
 
Publication

Chironomus riparius proteome responses to spinosad exposure

2020Journal article Open access
Simple item page
dc.contributor.authorMonteiro, Hugo R.
dc.contributor.authorPestana, João L.T.
dc.contributor.authorSoares, A.M.V.M.
dc.contributor.authorDevreese, Bart
dc.contributor.authorLemos, Marco F.L.
dc.date.accessioned2021-08-20T13:47:56Z
dc.date.available2021-08-20T13:47:56Z
dc.date.issued2020
dc.descriptionFunding: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UIDB/04292/2020, CESAM: UIDP/50017/2020+UIDB/50017/2020, FCT/MCTES through national funds, the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020, and project PROTEOME (PTDC/AAG-MAA/1302/2014) co-financed by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Program of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, and IF/01420/2015, respectively).
dc.description.abstractThe potential of proteome responses as early-warning indicators of insecticide exposure was evaluated using the non-biting midge Chironomus riparius (Meigen) as the model organism. Chironomus riparius larvae were exposed to environmentally relevant concentrations of the neurotoxic pesticide spinosad to uncover molecular events that may provide insights on the long-term individual and population level consequences. The iTRAQ labeling method was performed to quantify protein abundance changes between exposed and non-exposed organisms. Data analysis revealed a general dose-dependent decrease in the abundance of globin proteins as a result of spinosad exposure. Additionally, the downregulation of actin and a larval cuticle protein was also observed after spinosad exposure, which may be related to previously determined C. riparius life-history traits impairment and biochemical responses. Present results suggest that protein profile changes can be used as early warning biomarkers of pesticide exposure and may provide a better mechanistic interpretation of the toxic response of organisms, aiding in the assessment of the ecological effects of environmental contamination. This work also contributes to the understanding of the sublethal effects of insecticides in invertebrates and their molecular targets.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMonteiro HR, Pestana JLT, Soares AMVM, Devreese B, Lemos MFL. Chironomus riparius Proteome Responses to Spinosad Exposure. Toxics. 2020 Dec 11;8(4):117. doi: 10.3390/toxics8040117. PMID: 33322338; PMCID: PMC7768432.pt_PT
dc.identifier.doi10.3390/toxics8040117pt_PT
dc.identifier.issn2305-6304
dc.identifier.urihttp://hdl.handle.net/10400.8/6124
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPOCI-01-0145-FEDER-016773pt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationProTEoME - PROteomic Tools to assess Endocrine disruptiOn MEchanisms
dc.relationPROTEOME - PROTEOMIC TOOLS TO ASSESS ENDOCRINE DISRUPTION MECHANISTIC ECOTOXICITY
dc.relationAn integrative approach to assess the knock-on effects of psychiatric drugs in the freshwater environment
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAquatic insectspt_PT
dc.subjectEcotoxicoproteomicspt_PT
dc.subjectHemoglobinpt_PT
dc.subjectiTRAQpt_PT
dc.subjectNeurotoxic insecticidespt_PT
dc.titleChironomus riparius proteome responses to spinosad exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleProTEoME - PROteomic Tools to assess Endocrine disruptiOn MEchanisms
oaire.awardTitlePROTEOME - PROTEOMIC TOOLS TO ASSESS ENDOCRINE DISRUPTION MECHANISTIC ECOTOXICITY
oaire.awardTitleAn integrative approach to assess the knock-on effects of psychiatric drugs in the freshwater environment
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAAG-MAA%2F1302%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F80988%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01420%2F2015%2FCP1302%2FCT0016/PT
oaire.citation.issue4pt_PT
oaire.citation.titleToxicspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamInvestigador FCT
person.familyNameMonteiro
person.familyNameSoares
person.familyNameLemos
person.givenNameHugo Ricardo
person.givenNameAmadeu
person.givenNameMarco
person.identifier1119826
person.identifier996337
person.identifier.ciencia-id3415-56EB-358E
person.identifier.ciencia-idD512-6DD0-0E6F
person.identifier.orcid0000-0003-4913-1194
person.identifier.orcid0000-0003-0879-9470
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridC-7853-2012
person.identifier.ridA-8304-2008
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id56487254800
person.identifier.scopus-author-id57217386906
person.identifier.scopus-author-id7006042884
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd0845585-ad16-4565-adb0-63148ffb543c
relation.isAuthorOfPublication548bbf68-2cb9-444a-a52a-1303fe8def35
relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscoveryd0845585-ad16-4565-adb0-63148ffb543c
relation.isProjectOfPublication54154db1-7a14-4ce1-b54e-b3a01fcd44f5
relation.isProjectOfPublication9ad419d0-85f4-4d4b-9643-de9a80ebe442
relation.isProjectOfPublication0a413db3-a106-4c92-9220-99db283bff25
relation.isProjectOfPublication4e0a9ed1-4c4e-4869-a92e-b9c3b9527c79
relation.isProjectOfPublication46c02362-bd97-45d0-86c9-6bb4cb18a9eb
relation.isProjectOfPublication55e0ad86-600b-4ae8-bf7d-2d7143e3e172
relation.isProjectOfPublication.latestForDiscovery54154db1-7a14-4ce1-b54e-b3a01fcd44f5

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Monteiro et al_2020_Toxics.pdf
Size:
1.37 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

MARE - Artigos com peer review