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A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms

2012Journal article Restricted access
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dc.contributor.authorPereira, David M.
dc.contributor.authorVinholes, Juliana
dc.contributor.authorde Pinho, Paula Guedes
dc.contributor.authorValentao, Patricia
dc.contributor.authorMouga, Teresa
dc.contributor.authorTeixeira, Natercia
dc.contributor.authorAndrade, Paula B.
dc.date.accessioned2019-09-06T10:17:59Z
dc.date.available2019-09-06T10:17:59Z
dc.date.issued2012
dc.date.updated2019-09-04T14:55:15Z
dc.description.abstractIn this work a fast and simple multi-target gas chromatography–mass spectrometry (GC–MS) method for the simultaneous detection and absolute quantification of amino acids, fatty acids and sterols in marine organisms is proposed. The methodology was applied to the characterization of the echinoderm Marthasterias glacialis Linnaeus spiny sea star extracts. The main factors influencing the extraction of target compounds were evaluated by using different extraction procedures, solvent systems and temperature conditions and a comparison with a reference technique was performed. The most suitable procedure, capable of successfully extract the three classes of target compounds, was ethanol as solvent at 40 1C under magnetic stirring. Good analytical parameters were obtained since calibrations curves for the 40 compounds under analysis (15 amino acids, 16 fatty acids and 9 sterols) showed regression coefficients (r 2 ) ranging from 0.9844 to 0.9978, with low RSD (from 0.00 to 9.45%), and detection limits varying from 0.03 to 15.40 mg/L. The RSD values for intra- and interday variations studies were also good (RSDo13.5%, for both) and recoveries were higher than 92%. Variation in samples from different harvests and origins and their chemical composition during the year is reported. The fact that no previous treatment of samples is required can make this a useful technique for metabolite profiling in marine organisms, among others, both in biomedical and nutritional studies. Moreover, due to the fast and robust character of the proposed method it seems to be suitable for the implementation as routine analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationD.M. Pereira, et al., A gas chromatography–mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms, Talanta (2012), http://dx.doi.org/10.1016/j.talanta.2012.08.004pt_PT
dc.identifier.doi10.1016/j.talanta.2012.08.004
dc.identifier.otherWOS:000313773300052
dc.identifier.slugcv-prod-149974
dc.identifier.urihttp://hdl.handle.net/10400.8/4067
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - LA 6 - 2011-2012
dc.relation.ispartofTalantaen_US
dc.subjectMulti-target GC–MSpt_PT
dc.subjectMarine organismspt_PT
dc.subjectMarthasterias glacialis Linnaeuspt_PT
dc.subjectAmino acidspt_PT
dc.subjectFatty acidspt_PT
dc.subjectSterolspt_PT
dc.titleA gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organismspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F62663%2F2009/PT
oaire.citation.endPage400pt_PT
oaire.citation.startPage391pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume100pt_PT
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameMouga
person.givenNameTeresa
person.identifier.ciencia-idE312-FDCC-1ED8
person.identifier.orcid0000-0003-4849-8840
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidE312-FDCC-1ED8 | Teresa Mouga
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication901e8c7b-9818-4493-8e96-75d0c5ad718c
relation.isAuthorOfPublication.latestForDiscovery901e8c7b-9818-4493-8e96-75d0c5ad718c
relation.isProjectOfPublication4ef7a632-13d0-492f-ac17-5b6f22236ced
relation.isProjectOfPublication9f2dc794-a3e0-4621-abe3-9e242497cfdc
relation.isProjectOfPublication.latestForDiscovery4ef7a632-13d0-492f-ac17-5b6f22236ced

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