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A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms
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Orientador(es)
Resumo(s)
In this work a fast and simple multi-target gas chromatography–mass spectrometry (GC–MS) method
for the simultaneous detection and absolute quantification of amino acids, fatty acids and sterols in
marine organisms is proposed. The methodology was applied to the characterization of the echinoderm
Marthasterias glacialis Linnaeus spiny sea star extracts. The main factors influencing the extraction of
target compounds were evaluated by using different extraction procedures, solvent systems and
temperature conditions and a comparison with a reference technique was performed. The most suitable
procedure, capable of successfully extract the three classes of target compounds, was ethanol as solvent
at 40 1C under magnetic stirring. Good analytical parameters were obtained since calibrations curves
for the 40 compounds under analysis (15 amino acids, 16 fatty acids and 9 sterols) showed regression
coefficients (r
2
) ranging from 0.9844 to 0.9978, with low RSD (from 0.00 to 9.45%), and detection limits
varying from 0.03 to 15.40 mg/L. The RSD values for intra- and interday variations studies were also
good (RSDo13.5%, for both) and recoveries were higher than 92%. Variation in samples from different
harvests and origins and their chemical composition during the year is reported. The fact that no
previous treatment of samples is required can make this a useful technique for metabolite profiling in
marine organisms, among others, both in biomedical and nutritional studies. Moreover, due to the fast
and robust character of the proposed method it seems to be suitable for the implementation as routine
analysis.
Descrição
Palavras-chave
Multi-target GC–MS Marine organisms Marthasterias glacialis Linnaeus Amino acids Fatty acids Sterols
Contexto Educativo
Citação
D.M. Pereira, et al., A gas chromatography–mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms, Talanta (2012), http://dx.doi.org/10.1016/j.talanta.2012.08.004
Editora
Elsevier