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The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta
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Orientador(es)
Resumo(s)
The ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta’s ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC50 = 60.72 nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies.
Descrição
Palavras-chave
Acetylcholinesterase Capitela teleta Organophosphorous Insecticide Marine Sediment
Contexto Educativo
Citação
Isa D.L. Gomes, Marco F.L. Lemos, Amadeu M.V.M. Soares, Carlos Barata, Melissa Faria, The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta, Marine Pollution Bulletin, Volume 85, Issue 1, 2014, Pages 179-185, ISSN 0025-326X, https://doi.org/10.1016/j.marpolbul.2014.05.053
Editora
Elsevier BV
Licença CC
Sem licença CC