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The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta

2014-08-15Artigo científico Acesso restrito
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorGomes, Isa D. L.
dc.contributor.authorLemos, Marco F. L.
dc.contributor.authorSoares. Amadeu M. V. M.
dc.contributor.authorBarata, Carlos
dc.contributor.authorFaria, Melissa
dc.date.accessioned2026-06-26T14:46:16Z
dc.date.available2026-06-26T14:46:16Z
dc.date.issued2014-08-15
dc.description.abstractThe ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta’s ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC50 = 60.72 nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies.eng
dc.description.sponsorshipIsa D.L. Gomes acknowledges the “Consortium Erasmus Centro”, a partnership between European Union and the Portuguese national agency PROALV – Programa Aprendizagem ao Longo da Vida, for a partial grant to the internship at CSIC. Melissa Faria acknowledges the FCT fellowship SFRH/BPD/78342/2011, Portugal. Capitella teleta specimens were provided by Henriette Selck (Department of Life Sciences and Chemistry, University of Roskilde). The study was supported by European Funds through COMPETE, by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013 and by the Spanish MICINN grant and FEDER funds (CTM2011-30471-C02-01).
dc.identifier.citationIsa D.L. Gomes, Marco F.L. Lemos, Amadeu M.V.M. Soares, Carlos Barata, Melissa Faria, The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta, Marine Pollution Bulletin, Volume 85, Issue 1, 2014, Pages 179-185, ISSN 0025-326X, https://doi.org/10.1016/j.marpolbul.2014.05.053
dc.identifier.doi10.1016/j.marpolbul.2014.05.053
dc.identifier.issn0025-326X
dc.identifier.urihttp://hdl.handle.net/10400.8/16482
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationStrategic Project - LA 17 - 2013-2014
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0025326X14003440
dc.relation.ispartofMarine Pollution Bulletin
dc.rights.uriN/A
dc.subjectAcetylcholinesterase
dc.subjectCapitela teleta
dc.subjectOrganophosphorous
dc.subjectInsecticide
dc.subjectMarine
dc.subjectSediment
dc.titleThe use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teletaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPEst-C/MAR/LA0017/2013
oaire.awardTitleStrategic Project - LA 17 - 2013-2014
oaire.awardURIhttp://hdl.handle.net/10400.8/16264
oaire.citation.endPage185
oaire.citation.issue1
oaire.citation.startPage179
oaire.citation.titleMarine Pollution Bulletin
oaire.citation.volume85
oaire.fundingStreamConcurso para Financiamento de Projectos de IC&DT Estratégicos e de Interesse Público promovidos por Laboratórios Associados e Unidades de I&D - 2013 - COMPETE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLemos
person.givenNameMarco
person.identifier996337
person.identifier.ciencia-id971F-ACCA-C0D1
person.identifier.orcid0000-0001-9887-1864
person.identifier.ridF-7951-2011
person.identifier.scopus-author-id7006042884
relation.isAuthorOfPublicationf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isAuthorOfPublication.latestForDiscoveryf21e5540-df76-43e9-ad64-93edd70da1f1
relation.isProjectOfPublication0c167545-2fe7-4e35-9158-eb44bd29c56a
relation.isProjectOfPublication.latestForDiscovery0c167545-2fe7-4e35-9158-eb44bd29c56a

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