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Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds
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Orientador(es)
Resumo(s)
Cellular adhesion and proliferation inside three-dimensional synthetic scaffolds represent a major challenge in tissue engineering. Besides the surface chemistry of the polymers, it is well recognized that scaffold internal architecture, namely pore size/shape and interconnectivity, has a strong effect on the biological response of cells. This study reports for the first time how polycaprolactone (PCL) scaffolds with controlled micro-architecture can be effectively produced via bioextrusion and used to enhance the penetration of plasma deposited species. Low-pressure nitrogen-based coatings were employed to augment cell adhesion and proliferation without altering the mechanical properties of the structures.
X-ray photoelectron spectroscopy carried out on different sections of the scaffolds indicates a uniform distribution of nitrogen-containing groups throughout the entire porous structure. In vitro biological assays confirm that plasma deposition sensitively promotes the activity of Saos-2 osteoblast cells, leading to a homogeneous colonization of the PCL scaffolds.
Descrição
Palavras-chave
Tissue engineering Biofabrication Scaffolds Surface modification Osteoblast cells
Contexto Educativo
Citação
M. Domingos, F. Intranuovo, A. Gloria, R. Gristina, L. Ambrosio, P.J. Bártolo, P. Favia, Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds, Acta Biomaterialia, Volume 9, Issue 4, 2013, Pages 5997-6005, ISSN 1742-7061, https://doi.org/10.1016/j.actbio.2012.12.031.
Editora
Elsevier