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Detection of mcr-1 Gene in undefined Vibrio species isolated from clams

2022Journal article Open access
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dc.contributor.authorValdez, Christian
dc.contributor.authorCosta, Cátia
dc.contributor.authorSimões, Marco
dc.contributor.authorCarvalho, Carla C. C. R. De
dc.contributor.authorBaptista, Teresa
dc.contributor.authorCampos, Maria
dc.date.accessioned2022-02-28T14:59:42Z
dc.date.available2022-02-28T14:59:42Z
dc.date.issued2022
dc.date.updated2022-02-18T18:08:15Z
dc.descriptionFunding: This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 granted to MARE and grant agreement nº POCI‐01‐0247‐FEDER‐035234; AlgaValor, Portugal 2020 program.
dc.description.abstractThe increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic Vibrio are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filterfeeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive Vibrio spp. and 17 coliforms, three Vibrio spp. with MICs to colistin > 512 mg L−1 were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the mcr‐1 gene. Genotypic analysis for virulence genes in EB07V indicated chiA gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein‐coding gene sequences (recA, rpoB, groEL and dnaJ), grouped the isolates into the genus Vibrio but distinguished them as different from any known Vibrio spp.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationValdez, C.; Costa, C.; Simões, M.; de Carvalho, C.C.C.R.; Baptista, T.; Campos, M.J. Detection of mcr‐1 gene in undefined Vibrio species isolated from clams. Microorganisms 2022, 10, 394. https://doi.org/10.3390/ microorganisms10020394pt_PT
dc.identifier.doi10.3390/microorganisms10020394pt_PT
dc.identifier.issn2076-2607
dc.identifier.slugcv-prod-2719821
dc.identifier.urihttp://hdl.handle.net/10400.8/6723
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial resistancept_PT
dc.subjectColistinpt_PT
dc.subjectmcr‐1pt_PT
dc.subjectVibrio spp.pt_PT
dc.titleDetection of mcr-1 Gene in undefined Vibrio species isolated from clamspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameValdez Álvarez
person.familyNameGaspar Veríssimo Costa
person.familyNameDias Simões
person.familyNamede Carvalho
person.familyNameBaptista
person.familyNameCampos
person.givenNameChristian Andrés
person.givenNameCátia Filipa
person.givenNameMarco Aurélio
person.givenNameCarla
person.givenNameTeresa
person.givenNameMaria Jorge
person.identifier133107
person.identifier174191
person.identifier.ciencia-idD719-D4E8-0E9B
person.identifier.ciencia-idB61B-C0B9-89DF
person.identifier.ciencia-id1D1C-C217-F68A
person.identifier.ciencia-id541E-0EE5-090F
person.identifier.ciencia-idA61F-25C1-034A
person.identifier.ciencia-id2416-7867-2D40
person.identifier.orcid0000-0002-8558-5786
person.identifier.orcid0000-0001-7995-7304
person.identifier.orcid0000-0002-8142-3835
person.identifier.orcid0000-0003-1168-7461
person.identifier.orcid0000-0002-0883-0610
person.identifier.ridG-8176-2012
person.identifier.ridE-5591-2014
person.identifier.scopus-author-id56169816100
person.identifier.scopus-author-id55767069800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidA61F-25C1-034A | Teresa Maria Coelho Baptista
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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