Detection of mcr-1 Gene in undefined Vibrio species isolated from clams

Valdez, Christian; Costa, Cátia; Simões, Marco; Carvalho, Carla C. C. R. De; Baptista, Teresa; Campos, Maria

http://hdl.handle.net/10400.8/6723

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Authors

Valdez, Christian

Costa, Cátia

Simões, Marco

Carvalho, Carla C. C. R. De

Baptista, Teresa

Campos, Maria

Abstract(s)

The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic Vibrio are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filterfeeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive Vibrio spp. and 17 coliforms, three Vibrio spp. with MICs to colistin > 512 mg L−1 were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the mcr‐1 gene. Genotypic analysis for virulence genes in EB07V indicated chiA gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein‐coding gene sequences (recA, rpoB, groEL and dnaJ), grouped the isolates into the genus Vibrio but distinguished them as different from any known Vibrio spp.

Description

Funding: This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 granted to MARE and grant agreement nº POCI‐01‐0247‐FEDER‐035234; AlgaValor, Portugal 2020 program.

Keywords

Antimicrobial resistance Colistin mcr‐1 Vibrio spp.

URI

http://hdl.handle.net/10400.8/6723

Citation

Valdez, C.; Costa, C.; Simões, M.; de Carvalho, C.C.C.R.; Baptista, T.; Campos, M.J. Detection of mcr‐1 gene in undefined Vibrio species isolated from clams. Microorganisms 2022, 10, 394. https://doi.org/10.3390/ microorganisms10020394