ESTG - Artigos em revistas internacionais
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- 3D Photo-Fabrication for Tissue Engineering and Drug DeliveryPublication . Brás Pereira, Rúben Filipe; Bartolo, PauloThe most promising strategies in tissue engineering involve the integration of a triad of biomaterials, living cells, and biologically active molecules to engineer synthetic environments that closely mimic the healing milieu present in human tissues, and that stimulate tissue repair and regeneration. To be clinically effective, these environments must replicate, as closely as possible, the main characteristics of the native extracellular matrix (ECM) on a cellular and subcellular scale. Photo-fabrication techniques have already been used to generate 3D environments with precise architectures and heterogeneous composition, through a multi-layer procedure involving the selective photocrosslinking reaction of a light-sensitive prepolymer. Cells and therapeutic molecules can be included in the initial hydrogel precursor solution, and processed into 3D constructs. Recently, photo-fabrication has also been explored to dynamically modulate hydrogel features in real time, providing enhanced control of cell fate and delivery of bioactive compounds. This paper focuses on the use of 3D photo-fabrication techniques to produce advanced constructs for tissue regeneration and drug delivery applications. State-of-the-art photo-fabrication techniques are described, with emphasis on the operating principles and biofabrication strategies to create spatially controlled patterns of cells and bioactive factors. Considering its fast processing, spatiotemporal control, high resolution, and accuracy, photo-fabrication is assuming a critical role in the design of sophisticated 3D constructs. This technology is capable of providing appropriate environments for tissue regeneration, and regulating the spatiotemporal delivery of therapeutics.
- 3D printing of new biobased unsaturated polyesters by microstereo-thermal-lithographyPublication . Gonçalves, Filipa A. M. M.; Costa, Cátia S. M. F.; Fabela, Inês G. P.; Farinha, Dina; Faneca, Henrique; Simões, Pedro N.; Serra, Arménio C.; Bártolo, Paulo J.; Coelho, Jorge F. J.New micro three-dimensional (3D) scaffolds using biobased unsaturated polyesters (UPs) were prepared by microstereo-thermal-lithography (μSTLG). This advanced processing technique offers indubitable advantages over traditional printing methods. The accuracy and roughness of the 3D structures were evaluated by scanning electron microscopy and infinite focus microscopy, revealing a suitable roughness for cell attachment. UPs were synthesized by bulk polycondensation between biobased aliphatic diacids (succinic, adipic and sebacic acid) and two different glycols (propylene glycol and diethylene glycol) using fumaric acid as the source of double bonds. The chemical structures of the new oligomers were confirmed by proton nuclear magnetic resonance spectra, attenuated total reflectance Fourier transform infrared spectroscopy and matrix assisted laser desorption/ionization-time of flight mass spectrometry. The thermal and mechanical properties of the UPs were evaluated to determine the influence of the diacid/glycol ratio and the type of diacid in the polyester's properties. In addition an extensive thermal characterization of the polyesters is reported. The data presented in this work opens the possibility for the use of biobased polyesters in additive manufacturing technologies as a route to prepare biodegradable tailor made scaffolds that have potential applications in a tissue engineering area.
- 3D-printed multisampling holder for microcomputed tomography applied to life and materials science researchPublication . Vasconcelos, Isabel; Franco, Margarida; Pereira, Mário; Duarte, Isabel; Ginjeira, António; Alves, NunoThe aim of this work was to design, fabricate, test and validate a 3D-printed multisampling holder for multi-analysis by microcomputed tomography. Different raw materials were scanned by microcomputed tomography. The raw material chosen was used to fabricate the holder by 3D printing. To validate the multisampling holder, five teeth were filled with a high density-material and scanned in two ways: a single and a multisampling scan mode. For each tooth, the root canal filling volume, porosity volume, closed pore volume, and open pore volume were calculated and compared when the same tooth was scanned in the two sampling scan mode. ABSplus P430™ allowed a high transmission value (84.3 %), and then it was the polymeric material selected to fabricate the holder. In a single sampling scan mode, the scan duration for scanning five teeth was 87.42 min, contrasting with 21.51 min for a multisampling scan mode, which scanned five teeth at the same time. The scan duration time and the cost using a multisampling holder represented a reduction of 75 % and the data volume generated represented a reduction of 60 %. Comparing the two scan modes, the results also showed that the difference of root canal filling volume, porosity volume, closed pore volume, and open pore volume was not statistically significant (p > .05). The multisampling holder was validated to do multi-analysis by microcomputed tomography without significant loss of quantitative accuracy data, allowing a reduction in scan duration time, imaging cost, and data storage.
- A bi-criteria minimum spanning tree routing model for MPLS/overlay networksPublication . José Craveirinha; João Clímaco; Lúcia Martins; Silva, Carlos Gomes da; Nuno FerreiraThe MPLS platform enables the implementation of advanced multipath and multicast routing schemes. This work develops and analyses the performance of a new bicriteria minimum spanning tree model intended for routing broadcast messages in MPLS networks or constructing treebased overlay networks. The aim of the model is to obtain spanning trees which are compromise solutions with respect to two important traffic engineering metrics: load balancing cost and average delay bound. An exact solution to the formulated bi-criteria optimization problem is presented, which is based on an algorithm that enables the computation of the set of supported non-dominated spanning trees. An application model and a set of experiments on randomly generated Internet type topologies will also be presented. Finally a network performance analysis of the model considering three network performance metrics will be shown.
- Additive manufactured porous biomaterials targeting orthopedic implants: A suitable combination of mechanical, physical and topological propertiesPublication . Bartolomeu, F.; Dourado, N.; Pereira, F.; Alves, N.; Miranda, G.; Silva, F. S.Orthopedic implants are under incessant advancement to improve their interactions with surrounding bone tissue aiming to ensure successful outcomes for patients. A successful biological interaction between implant and surrounding bone depends on the combination of mechanical, physical and topological properties. Hence, Ti6Al4V cellular structures appear as very promising solutions towards the improvement of conventional orthopedic implants. This work addresses a set of fundamental tools that allow improving the design of Ti6Al4V cellular structures produced by Selective Laser Melting (SLM). Three-point bending tests were carried out to estimate the elastic modulus of the produced structures. Morphological analysis allowed to evaluate the dimensional differences that were noticed between the model CAD and the SLM structures. Finite element models (adjusted CAD) were constructed with the experimentally obtained dimensions to replicate the mechanical response of the SLM structures. Linear correlations were systematically found for the dimensions of the SLM structures as a function of the designed model CAD dimensions. This has also been observed for the measured porosities as a function of the designed CAD models. This data can be used in further FE analyses as design guidelines to help engineers fabricating near-net-shape SLM Ti6Al4V cellular structures. Besides, polished and sandblasted surface treatments performed on the Ti6Al4V cellular structures allowed to obtain suitable properties regarding roughness and wettability when compared to as-produced surfaces. The capillarity tests showed that all the analyzed Ti6Al4V structures are able to transport fluid along its structure. The cell viability tests demonstrate Ti6Al4V cellular structures SLM produced did not release toxic substances to the medium, indicating that these structures can assure a suitable environment for cells to proliferate and attach. This study proposes a design methodology for Ti6Al4V cellular structures, that owe suitable mechanical properties but also provide a proper combination of porosity, roughness, wettability, capillarity and cell viability, all of them relevant for orthopedic applications. A Ti6Al4V cellular structured hip implant prototype gathering the suitable features addressed in this study was successfully SLM-produced.
- Advances in phytochemical delivery systems for improved anticancer activityPublication . Lagoa, Ricardo; Silva, João; Rodrigues, Joaquim Rui; Bishayee, AnupamNatural compounds have significant anticancer pharmacological activities, but often suffer from low bioavailability and selectivity that limit therapeutic use. The present work critically analyzes the latest advances on drug delivery systems designed to enhance pharmacokinetics, targeting, cellular uptake and efficacy of anticancer phytoconstituents. Various phytochemicals, including flavonoids, resveratrol, celastrol, curcumin, berberine and camptothecins, carried by liposomes, nanoparticles, nanoemulsions and films showed promising results. Strategies to avoid drug metabolism, overcome physiological barriers and achieve higher concentration at cancer sites through skin, buccal, nasal, vaginal, pulmonary and colon targeted delivery are presented. Current limitations, challenges and future research directions are also discussed.
- AlineaGA - a genetic algorithm with local search optimization for multiple sequence alignmentPublication . Silva, Fernando José Mateus da; Pérez, Juan Manuel Sánchez; Pulido, Juan Antonio Gómez; Rodríguez, Miguel A. VegaThe alignment and comparison of DNA, RNA and Protein sequences is one of the most common and important tasks in Bioinformatics. However, due to the size and complexity of the search space involved, the search for the best possible alignment for a set of sequences is not trivial. Genetic Algorithms have a predisposition for optimizing general combinatorial problems and therefore are serious candidates for solving multiple sequence alignment tasks. Local search optimization can be used to refine the solutions explored by Genetic Algorithms. We have designed a Genetic Algorithm which incorporates local search for this purpose: AlineaGA. We have tested AlineaGA with representative sequence sets of the globin family. We also compare the achieved results with the results provided by T-COFFEE.
- An alternative method to produce metal/plastic hybrid components for orthopedics applicationsPublication . Silva, M.; Mateus, A.; Oliveira, D.; Malça, C.The demand for additive processes that provide components with high technological performance became overriding regardless of the application area. For medical applications, the orthopedics field—multimaterial orthoses and splints—can clearly benefit from direct additive manufacturing using a hybrid process instead of the traditional handmade manufacturing, which is slow, expensive, inaccurate, and difficult to reproduce. The ability to provide faster better orthoses, using innovative services and technologies, resulting in lower recovery times, reduced symptoms, and improved functional capacity, result in a significant impact on quality of life and the well-being of citizens. With these purposes, this work presents an integrate methodology, that includes the tridimensional (3D) scanning, 3D computer-aided design modeling, and the direct digital manufacturing of multimaterial orthoses and splints. Nevertheless, additive manufacturing of components with functional gradients, multimaterial components, e.g. metal/plastic is a great challenge since the processing factors for each one of them are very different. This paper proposes the addition of two advanced additive manufacturing technologies, the selective laser melting and the stereolithography, enabling the production of a photopolymerization of the polymer in the voids of a 3D metal mesh previously produced by selective laser melting. Based on biomimetic structures concept, this mesh is subject to a previous design optimization procedure in order to optimize its geometry, minimizing the mass involved and evidencing increased mechanical strength among other characteristics. A prototype of a hybrid additive manufacturing device was developed and its flexibility of construction, geometrical freedom, and different materials processability is demonstrated through the case study—arm orthosis—presented in this work.
- Analysis of fatigue crack propagation in laser sintering metalPublication . Borrego, L. F. P.; Antunes, F. V.; Ferreira, J. A. M.; Costa, J. D.; Capela, C.Laser sintering metal has recently been used in the manufacture of components for different applications like aerospace or medicine. The approach to engineering design based on the cracks propagation assumption applying the concepts of linear elastic fracture mechanics (LEFM) is commonly used for aerospace engineering. However, fatigue crack propagation is linked to irreversible and non-linear mechanisms at the crack tip, therefore LEFM parameters can be successfully replaced by non-linear crack parameters, namely the plastic CTOD. A model linking da/dN with plastic CTOD is proposed here to characterize fatigue crack propagation. A comparison is made with other materials showing that for the same plastic CTOD the laser sintering material has a relatively large crack growth rate.
- Analysis of friction in the ejection of thermoplastic mouldingsPublication . Correia, Mário Simões; Miranda, Antonio Sousa; Oliveira, Marta Cristina; Capela, Carlos Alexandre; Pouzada, Antonio SergioThe ejection force of injection-moulded thermoplastics depends on the contact conditions at the moment of ejection. Replication of the polymer part surface occurs onto the mould surface during injection of the melt. Ejection takes place in a very short time, hence the static coefficient of friction must be considered for modelling the ejection process. To understand the contribution of the mechanisms involved in the friction during the ejection stage, a mixed approach was followed: analytical simulation of the ploughing, numerical simulation of the deformation, and experimental inference of the adhesion. The relevance of roughness, temperature and contact pressure in the coefficient of friction was evidenced.