Percorrer por autor "Rodrigues, Ana"
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- Algae from Portuguese Coast Presented High Cytotoxicity and Antiproliferative Effects on an In vitro Model of Human Colorectal CancerPublication . Alves, Celso; Pinteus, Susete; Rodrigues, Ana; Horta, André; Pedrosa, RuiBackground: The marine environment has shown to be an interesting source of new antitumor agents, representing an important tool in cancer research. Objective: The aim of this study was to evaluate the antitumor activities of 12 algae from Peniche coast (Portugal) on an in vitro model of human colorectal cancer (Caco‑2 cells). Materials and Methods: The antitumor potential was accessed by evaluating Caco‑2 cell’s viability and proliferation through the 3‑[4, 5‑dimethylthiazol‑2‑yl]‑2, 5‑diphenyl tetrazolium bromide and calcein‑AM methods. Results: The dichloromethane extracts of Asparagopsis armata and Sphaerococcus coronopifolius induced the highest decrease on cell’s viability (1 mg/mL; 24 h), 98.96% ± 0.39% and 98.08% ± 0.89%, respectively, followed by the methanolic extracts of S. coronopifolius (96.47% ± 1.26%) and A. armata (92.68% ± 1.17%). Regarding cell proliferation, the highest decrease of Caco‑2 cell’s proliferation (1 mg/mL; 24 h) was induced by the dichloromethane extract of A. armata (100% ± 0.48%), S. coronopifolius (99.04 ± 0.51%), and Plocamium cartilagineum (95.05% ± 1.19%). The highest potency was shown by the dichloromethane extract of S. coronopifolius in both, cytotoxicity and antiproliferative tests, with an IC50 of 21.3 and 36.5 µg/mL, respectively. Conclusion: The extracts of A. armata and S. coronopifolius are promising sources of new bioactive molecules with application in cancer therapeutics.
- Contabilidade para a sustentabilidade – Uma análise dos principais trabalhos publicados nas TOP 10 do SCIMAGO Journal RankPublication . Casca, António; Rodrigues, Ana; Eugénio, TeresaObjetivo: Identificação da produção científica em contabilidade ambiental e social no período de 2009 a 2015, nas revistas de contabilidade consideradas Top 10 de acordo com a classificação de Scimago Journal Rank (SJR). Metodologia: Análise de conteúdo dos artigos após uma categorização pré-definida seguindo a metodologia utilizada por Bianchi et al. (2011). Conclusões e Investigação futura: A análise realizada permitiu-nos concluir que as revistas que publicam mais nesta área de investigação são a Accounting, Auditing and Accountability Journal (AAAJ) e Accounting, Organizations and Society (AOS). No que respeita à base teórica subjacente a grande parte destes trabalhos é a teoria da legitimidade. Aplicar metodologias distintas das utilizadas na generalidade dos estudos analisados, de modo a procurar utilizar amostras diferentes, nomeadamente, as pequenas e médias empresas. Por último, a relevância desta área irá impor um alargamento do âmbito das investigações, passando a integrar variáveis mais qualitativas relacionadas com o governo das sociedades, bem como uma maior preocupação com o relatório integrado. Contributos: O presente trabalho é um contributo para a investigação em contabilidade ambiental e social identificando futuras linhas de investigação.
- Cut-off for the legal ages in the Portuguese Population by Third Maturity Index: Measures of AccuracyPublication . Pereira, Cristiana Palmela; Rodrigues, Ana; Santos, Adriana; Salvado, Francisco; Santos, Rui; Cameriere, RobertoObjectives: The aim of this study is to estimate the cut-off points for the Portuguese legal ages through the I3M, and to compare them with the methods of Demirjian, Nolla and Moorrees. Design: The lower third molars were analyzed on 348 orthopantomography's aged between 12 and 23 years in a Portuguese Population. The images were analyzed by ImageJ and the cut-off points were calculated for the respective legal ages of 14, 16, 18 and 21 years old. Results: The correlation between age and the I3M was 0.862, whereas with Demirjian's stadiums, Nolla's stages, and Moorrees’ stages the correlation coefficients were 0.863, 0.842 and 0.844, respectively. For the cut-off point of 0.08 for the age of 18, a sensitivity of 78.99 %, specificity of 93.48 %, an accuracy of 88.54 %, a positive predictive value of 86.24 % and a posteriori Bayes probability of 92.82 %. The cut-off points established for the ages of 14, 16, 18 and 21 years for the Portuguese population achieved an accuracy of 83.67 %, 85.67 %, 88.54 % and 87.11 %, respectively. Conclusion: Demirjian's method and Cameriere's method attain quite similar results. In certain ages, the methods of Nolla and Moorrees show higher sensitivity or higher specificity when compared to Cameriere's method, however this last method is more stable in terms of reliability and more suitable to use in the Portuguese population.
- Dental Age Assessment by I2M and I3M: Portuguese Legal Age Thresholds of 12 and 14 Year OldsPublication . Augusto, Diana; Pereira, Cristiana Palmela; Rodrigues, Ana; Cameriere, Roberto; Salvado, Francisco; Santos, RuiObjective: Better understanding of dental age assessment may help in cases of age estimation in Forensic Clinics. The first aim was to provide essential information on method reliability for upcoming studies using dental age assessment by second molar index (I2M), and third molar index (I3M) for age estimation on legal ages of 12 - 14 years. The second aim was to document forensic method outcomes of the Demirjian method which has already been used in forensic clinic. Material and methods: Two samples were used for this purpose: for I2M, 633 orthopantomographs (270 females / 363 males), the age range from 7 to 17 years and for I3M, 471 orthopantomographs (253 females / 218 males), the age range from 10 to 23 years, from the database population of Lisbon North University Hospital Center, approved by the Ethic Committee. Results: The I3M cut-off point (1.133) for 12- year-olds obtained better results than the cut-off point stated by the I2M (0.135). Besides, I2M cut-off point (0.001) for 14- year- olds showed better results when compared with the cut-off point (0.705) established by the I3M. Both methods are reliable for the legal age thresholds of 12 and 14 years. However, using I2M and I3M allows us to vary the cut-off value to privilege sensitivities or specificity, depending on which is more appropriate to the intended application. Conclusions: The accuracy (88.94%) of I3M obtained better results for the 12- year- old cut-off point (1.133) and the accuracy (90.21%) of the I2M performed better for the 14- year- old cut-off point (0.001).
- Forensic age estimation using new models of mathematical regression formula constructed with molar indexes: dental age assessmentPublication . Pereira, Cristiana Palmela; Rodrigues, Ana; Augusto, Diana; Santos, Adriana; Salvado, Francisco; Santos, Rui; Cameriere, RobertoIntroduction: In forensic science, age estimation for identification purposes of living and deceased individuals, is of great importance. It is crucial for humanitarian reasons in both civil and criminal cases. Objectives: The aim of this study was to contribute to the process of forensic age estimation using dental age assessment with mineralization stages of Demirjian method, and maturity indexes of permanent mandibular second (I2M) and third molars (I3M). Material and methods: Two samples were used from a Portuguese population; for I2M, 591 orthopantomography’s aged between 7 and 15 years and for I3M, 350 orthopantomography’s aged between 12 and 23 years. Age estimation was obtained using linear regression models, each model was estimated with all observations and without observations, in which I2M or I3M = 0. Results: The results of ICC for intra and interobserver validation varied between 0.608 and 0.999 for both indexes. Pearson correlation coefficient between each index and chronological age showed that all indexes were significantly negatively correlated with chronological age. The standard error of estimate and the mean absolute error were continually low without patients with null index. Conclusions: Application of I2M is reproducible in a Portuguese population for medicallegal application in a population aged 7 to 15 years, when the corresponding index is positive. Third molar must be used only if its index is positive. The inclusion of Demirjian staging in both models significantly increases accuracy.
- Produção e caracterização da capacidade antioxidante de gelo suplementado com extrato de Fucus spiralisPublication . Rodrigues, AnaO peixe fresco é suscetível à deterioração causada por reações microbiológicas e químicas. A deterioração lipídica ocorre facilmente e limita a vida de prateleira de peixes gordos durante o armazenamento. Compostos bioativos, como compostos fenólicos presentes em algas como a Fucus spiralis com elevada capacidade antioxidante podem ser utilizadas para retardar ou inibir os processos de oxidação lipídica no pescado fresco. No presente trabalho, avaliou-se a eficiência da utilização Algelo, gelo suplementado com extrato da alga Fucus spiralis, na estabilidade oxidativa da sardinha (Sardina pilchardus). Os compostos bioativos foram extraídos da alga Fucus spiralis com recurso a solventes polares, a água e o etanol com diferentes proporções. Foi avaliada a capacidade antioxidante das diferentes extrações e do Algelo através de dois métodos, a Quantificação Total de Polifenóis (QTP) e a capacidade de redução do radical 1, 1-Diphenyl2-picrylhydrazyl (DPPH). A estabilidade oxidativa da sardinha foi avaliada pela determinação do índice do ácido tiobarbitúrico (TBA) e pela determinação dos parâmetros de cor L* e a*. As extrações com etanol demonstraram elevada capacidade de captação de compostos fenólicos, sendo esta capacidade independente da % de etanol. As soluções resultantes da extração da alga Fucus spiralis demonstraram estabilidade antioxidante durante 60 dias. No entanto, o Algelo mostrou pouca capacidade para reter os compostos antioxidantes, sendo estes maioritariamente lixiviados na água de degelo. Foram feitas duas concentrações da solução utilizada para produção de Algelo, 100 / e 500 mg de eq. de ácido gálico /l, de modo a avaliar o efeito deste na oxidação lipídica dos filetes de sardinha. Apenas a concentração de Algelo mais elevada mostrou reduzir a oxidação lipídica da sardinha às 48 horas. Os parâmetros de cor L* e a* da sardinha não foram alterados pela presença do Algelo. Como conclusão, podemos afirmar que a solução de gelo suplementado com extratos de Fucus spiralis tem elevada capacidade antioxidante, sendo estável ao longo do tempo. O Algelo não se mostrou muito eficaz na inibição da oxidação lipídica da sardinha, facto que poderá estar relacionado com a perda da capacidade antioxidante do Algelo à superfície, com perda das moléculas antioxidante na água de degelo.
- Rural tourism associated with agriculture as an economic alternative for the farmersPublication . Calado, Luísa; Rodrigues, Ana; Silveira, Paulo; Dentinho, TomazThis paper aims at presenting a possible approach to identify the best sites for rural tourism and also to analyze the synergies between agriculture and cultural heritage in Azores, in order to be incorporated in the full range of management concerns into private and public decision‑making. The following territorial aptitudes for alternative were used to simulate this exercise: urban, touristic, horticulture, agricultural, cattle and forestry. Soil potential was defined in a number of classes from I to VII. The best hypothetical sites for rural tourism were defined using Geographical Information Systems (GIS).