Algae from Portuguese Coast Presented High Cytotoxicity and Antiproliferative Effects on an In vitro Model of Human Colorectal Cancer

Alves, Celso; Pinteus, Susete; Rodrigues, Ana; Horta, André; Pedrosa, Rui

http://hdl.handle.net/10400.8/4973

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Abstract(s)

Background: The marine environment has shown to be an interesting source of new antitumor agents, representing an important tool in cancer research. Objective: The aim of this study was to evaluate the antitumor activities of 12 algae from Peniche coast (Portugal) on an in vitro model of human colorectal cancer (Caco‑2 cells). Materials and Methods: The antitumor potential was accessed by evaluating Caco‑2 cell’s viability and proliferation through the 3‑[4, 5‑dimethylthiazol‑2‑yl]‑2, 5‑diphenyl tetrazolium bromide and calcein‑AM methods. Results: The dichloromethane extracts of Asparagopsis armata and Sphaerococcus coronopifolius induced the highest decrease on cell’s viability (1 mg/mL; 24 h), 98.96% ± 0.39% and 98.08% ± 0.89%, respectively, followed by the methanolic extracts of S. coronopifolius (96.47% ± 1.26%) and A. armata (92.68% ± 1.17%). Regarding cell proliferation, the highest decrease of Caco‑2 cell’s proliferation (1 mg/mL; 24 h) was induced by the dichloromethane extract of A. armata (100% ± 0.48%), S. coronopifolius (99.04 ± 0.51%), and Plocamium cartilagineum (95.05% ± 1.19%). The highest potency was shown by the dichloromethane extract of S. coronopifolius in both, cytotoxicity and antiproliferative tests, with an IC50 of 21.3 and 36.5 µg/mL, respectively. Conclusion: The extracts of A. armata and S. coronopifolius are promising sources of new bioactive molecules with application in cancer therapeutics.

Description

This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through Red2Discovery Project (PTDC/MAR‑BIO/6149/2014), co‑financed by COMPETE (POCI‑01‑0145‑FEDER‑016791), and Strategic Project UID/MAR/04292/2013 granted to MARE and the support of the FP7 EU project “BAMMBO: Sustainable Production of Biologically Active Molecules of Marine Based Origin” (FP7 n 265896/http//www.bammbo. eu). C.A, S.P. and A.H. are financially supported by a grant from FCT Fundação para a Ciência e Tecnologia (SFRH/BD/97764/2013, SFRH/ BD/96203/2013 and SFRH/BD/120250/2016, respectively).

Keywords

Bioactive compounds Caco‑2 cells Marine natural products Red macroalgae Seaweed

URI

http://hdl.handle.net/10400.8/4973

Citation

Alves C, Pinteus S, Rodrigues A, Horta A, Pedrosa R. Algae from Portuguese Coast Presented High Cytotoxicity and Antiproliferative Effects on an In vitro Model of Human Colorectal Cancer. Phcog Res 2018;10:24-30.