Percorrer por autor "Domingos, M."
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- BioCell Printing : Integrated automated assembly system for tissue engineering constructsPublication . Bártolo, P.; Domingos, M.; Gloria, A.; Ciurana, J.The production methodology of 3D constructs for tissue regeneration is usually a complex discontinuous process involving three different stages: (1) production of 3D matrices; (2) matrix sterilisation and cell seeding; (3) in vitro dynamic cell culture. This paper presents a novel automated bench-top manufacturing system called BioCell Printing, designed for the integrated, continuous and fully automated production and in vitro dynamic culture of tissue engineering constructs. The BioCell aims at the rapid production of tissue-engineered substitutes with low risk of contamination, increasing the chances of direct clinical application.
- Effect of process parameters on the morphological and mechanical properties of 3D Bioextruded poly(ε‐caprolactone) scaffoldsPublication . Domingos, M.; Chiellini, F.; Gloria, A.; Ambrosio, L.; Bártolo, P.; Chiellini, E.Purpose – This paper aims to report a detailed study regarding the influence of process parameters on the morphological/mechanical properties of poly(1-caprolactone) (PCL) scaffolds manufactured by using a novel extrusion-based system that is called BioExtruder. Design/methodology/approach – In this study the authors focused investigations on four parameters, namely the liquefier temperature (LT), screw rotation velocity (SRV), deposition velocity (DV) and slice thickness (ST). Scaffolds were fabricated by employing three different values of each parameter. Through a series of trials, scaffolds were manufactured varying iteratively one parameter while maintaining constant the other ones. The morphology of the structures was investigated using a scanning electron microscope (SEM), whilst the mechanical performance was assessed though compression tests. Findings – Experimental results highlight a direct influence of the process parameters on the PCL scaffolds properties. In particular, DV and SRV have the highest influence in terms of road width (RW) and consequently on the porosity and mechanical behaviour of the structures. Research limitations/implications – The effect of process and design parameters on the biological response of scaffolds is currently under investigation. Originality/value – The output of this work provides a major insight into the effect of process parameters on the morphological/mechanical properties of PCL scaffolds. Moreover, the potential and feasibility of this novel extrusion-based system open a new opportunity to study how structural features may influence the characteristics and performances of the scaffolds, enabling the development of integrated biomechanical models that can be used in CAD systems to manufacture customized structures for tissue regeneration.
- Evaluation of in vitro degradation of PCL scaffolds fabricated via BioExtrusion. Part 1: Influence of the degradation environmentPublication . Domingos, M.; Chiellini, F.; Cometa, S.; De Giglio, E.; Grillo-Fernandes, E.; Bártolo, P.; Chiellini, E.One of the most promising approaches in tissue engineering (TE) comprises the development of 3D porous scaffolds which are able to promote tissue regeneration. Biocompatible and biodegradable poly(e-caprolactone) (PCL) structures are increasingly used as temporary extra-cellular matrices for bone tissue engineering. To ensure an appropriate bone restoration over the long term, the selected material must have a degradation rate that match the in-growth of new bone. The in vivo process, by which the scaffold degrades and is resorbed transferring the load and function back to the host tissue, is complex. Consequently, an appropriate preliminary in vitro study is required. A novel extrusion-based technology called BioExtruder was used to produce PCL porous scaffolds made with layers of directionally aligned microfilaments. The in vitro degradation behaviour in both simulated body fluid (SBF) and phosphate buffer solution (PBS) were investigated over 6 months. The characterization of the degradation behaviour of the structures was performed at specific times by evaluating changes in the average molecular weight, the weight loss and its thermal properties. Morphological and surface chemical analyses were also performed using a Scanning Electron Microscopy (SEM) and an X-ray Photoelectron Spectroscopy (XPS), respectively.
- Polymers, Biomanufacturing and Regenerative MedicinePublication . Mitchell, Geoffrey R.; Domingos, M.; Bártolo, Paulo J.Polymers have a major contribution to make in the field of regenerative medicine in order to develop and deploy biological substitutes that restore, maintain, or improve tissue function. There are realistic opportunities to create tissues using an engineered extracellular matrix or scaffold and biologically active molecules. A number of different approaches to preparing scaffolds have been explored and the requirements for materials and personalization identified. We review the methodologies and materials available and report on recent work at the CDRSP to integrate and provide control of the processes to provide a realistic biomanufacturing process.