Percorrer por autor "Bartolo, Paulo"
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- 3D bioprinting of photocrosslinkable hydrogel constructsPublication . Brás Pereira, Rúben Filipe; Bartolo, PauloThree‐dimensional (3D) bioprinting comprises a group of biofabrication technologies for the additive manufacturing of 3D constructs by precisely printing biocompatible materials, cells and biochemicals in predesigned spatial positions. These technologies have been successfully applied to fabricate biodegradable 3D constructs with intricate architectures and heterogeneous composition, assuming a pivotal role in the field of tissue engineering. However, the full implementation of bioprinting strongly depends on the development of novel biomaterials exhibiting fast crosslinking schemes and appropriate printability, cell‐compatibility and biomechanical properties. Photocrosslinkable hydrogels are attractive materials for bioprinting as they provide fast polymerization under cell‐compatible conditions and exceptional spatiotemporal control over the gelation process. Photopolymerization can also be performed during the bioprinting to promote the instantaneous formation of hydrogel with high well‐defined architecture and structural stability. In this review paper, we summarize the most recent developments on bioprinting of photocrosslinkable biodegradable hydrogels for tissue engineering, focusing on the chemical modification strategies and the combination of photocrosslinking reactions with other gelation modalities.
- 3D Photo-Fabrication for Tissue Engineering and Drug DeliveryPublication . Brás Pereira, Rúben Filipe; Bartolo, PauloThe most promising strategies in tissue engineering involve the integration of a triad of biomaterials, living cells, and biologically active molecules to engineer synthetic environments that closely mimic the healing milieu present in human tissues, and that stimulate tissue repair and regeneration. To be clinically effective, these environments must replicate, as closely as possible, the main characteristics of the native extracellular matrix (ECM) on a cellular and subcellular scale. Photo-fabrication techniques have already been used to generate 3D environments with precise architectures and heterogeneous composition, through a multi-layer procedure involving the selective photocrosslinking reaction of a light-sensitive prepolymer. Cells and therapeutic molecules can be included in the initial hydrogel precursor solution, and processed into 3D constructs. Recently, photo-fabrication has also been explored to dynamically modulate hydrogel features in real time, providing enhanced control of cell fate and delivery of bioactive compounds. This paper focuses on the use of 3D photo-fabrication techniques to produce advanced constructs for tissue regeneration and drug delivery applications. State-of-the-art photo-fabrication techniques are described, with emphasis on the operating principles and biofabrication strategies to create spatially controlled patterns of cells and bioactive factors. Considering its fast processing, spatiotemporal control, high resolution, and accuracy, photo-fabrication is assuming a critical role in the design of sophisticated 3D constructs. This technology is capable of providing appropriate environments for tissue regeneration, and regulating the spatiotemporal delivery of therapeutics.
- Biomanufacturing for tissue engineering: Present and future trendsPublication . Bartolo, Paulo; Chua, C. K.; Almeida, Henrique de Amorim; Chou, S. M.; Lim, A. S. C.Tissue engineering, often referred to as regenerative medicine and reparative medicine, is an interdisciplinary field that necessitates the combined effort of cell biologists, engineers, material scientists, mathematicians, geneticists, and clinicians toward the development of biological substitutes that restore, maintain, or improve tissue function. It has emerged as a rapidly expanding approach to address the organ shortage problem and comprises tissue regeneration and organ substitution. Cells placed on/or within constructs is the most common strategy in tissue engineering. Successful cell seeding depends on fast attachment of cell to scaffolds, high cell survival and uniform cell distribution. The seeding time is strongly dependent on the scaffold material and architecture. Scaffolds provide an initial biochemical substrate for the novel tissue until cells can produce their own extra-cellular matrix (ECM). Thus scaffolds not only define the 3D space for the formation of new tissues, but also serve to provide tissues with appropriate functions. These scaffolds are often critical, both in vivo (within the body) or in vitro (outside the body) mimicking in vivo conditions. Additive fabrication processes represent a new group of non-conventional fabrication techniques recently introduced in the biomedical engineering field. In tissue engineering, additive fabrication processes have been used to produce scaffolds with customised external shape and predefined internal morphology, allowing good control of pore size and pore distribution. This article provides a comprehensive state-of-the-art review of the application of biomanufacturing additive processes in the field of tissue engineering. New and moving trends in biomanufacturing technologies and the concept of direct cell-printing technologies are also discussed.
- Biomedical production of implants by additive electro-chemical and physical processesPublication . Bartolo, Paulo; Kruth, Jean-Pierre; Silva, Jorge; Levy, Gideon; Malshe, Ajay; Rajurkar, Kamlakar; Mitsuishi, Mamoru; Ciurana, Joaquim; Leu, MingBiomanufacturing integrates life science and engineering fundamentals to produce biocompatible products enhancing the quality of life. The state-of-the-art of this rapidly evolving manufacturing sector is presented and discussed, in particular the additive electrical, chemical and physical processes currently being applied to produce synthetic and biological parts. This fabrication strategy is strongly materialdependent, so the main classes of biomaterials are detailed. It is explained the potential to process composite materials combining synthetic and biological materials, such as cells, proteins and growth factors, as well the interdependences between materials and processes. The techniques commonly used to increase the bioactivity of clinical implants and improve the interface characteristics between biological tissues and implants are also presented.
- EditorialPublication . Bartolo, Paulo; Chua, C.K.In this issue of Virtual and Physical Prototyping, there are three Virtual Prototyping papers and two Physical Prototyping papers. Interestingly, three of the five papers relate to the area of bone tissue engineering, an area which is of great and current research interest to readers.
- EditorialPublication . Bartolo, Paulo; Chua, C. K.
- EditorialPublication . Bartolo, Paulo; Chua, C. K.
- EditorialPublication . Bartolo, Paulo; Chua, C. K.
- EditorialPublication . Bartolo, Paulo; Chua, C. K.Welcome to a brand new year of 2010! In this editorial, we bring you the latest update concerning rapid prototyping technologies,which of coursemany of you are aware, include many terminologies such as layer manufacturing and solid freeform fabrication, amongst others. The longstanding need to standardize the terminologies within the rapid prototyping andmanufacturing industry has nowbeenmetwith a new ASTMInternational standard, ASTMF2792, Terminology for Additive Manufacturing Technologies. The new document is the first approved standard under the jurisdiction of ASTM Committee F42 on Additive Manufacturing Technologies, which was formed in 2009.
- EditorialPublication . Bartolo, Paulo; Chua, C. K.
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