BSA-PEG Hydrogel: A Novel Protein-Ligand Binding 3D Matrix

Coelho, Carlos D. F.; Jesus, João A.; Vaz, Daniela C.; Lagoa, Ricardo; Moreno, Maria João

http://hdl.handle.net/10400.8/7803

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Abstract(s)

Hydrogel materials have good biomimetic properties and high potential for biomedical and bioanalytical applications. In this work, a hydrogel of serum albumin crosslinked with poly-(ethylene glycol) was prepared and characterized for its water content, protein structure and stability. The ability of the hydrogel to bind small molecule ligands with different hydrophobicity was evaluated using a homologous series of amphiphiles (NBD-Cn, n = 4, 6 and 8) and the calculated binding affinities were similar to that of free protein in solution. Overall, the results indicate this type of hydrogel system as a convenient tool for studying the binding of xenobiotics to tissue proteins.

Keywords

Ligand binding Serum albumin Hydrogel Protein structure

URI

http://hdl.handle.net/10400.8/7803

Citation

Coelho, C.D.F.; Jesus, J.A.; Vaz, D.C.; Lagoa, R.; Moreno, M.J. BSA-PEG Hydrogel: A Novel Protein-Ligand Binding 3D Matrix. Med. Sci. Forum 2022, 11, 1. https://doi.org/10.3390/ BiTaP-12878