Repository logo
 
Publication

Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model

2020Journal article Restricted access
Simple item page
dc.contributor.authorSilva, Joana
dc.contributor.authorAlves, Celso
dc.contributor.authorPinteus, Susete
dc.contributor.authorMendes, Susana
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2020-08-28T14:09:50Z
dc.date.available2020-08-28T14:09:50Z
dc.date.issued2020
dc.descriptionFunding This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/2020 granted to MARE—Marine and Environmental Sciences Centre, through Red2Discovery project (PTDC/MAR-BIO/6149/2014), co-fnanced by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015-LISBOA-01-0145-FEDER-016405) and through CrossAtlantic project (PTDC/BIA-OUT/29250/2017), co-fnanced by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/BD/103255/2014).
dc.description.abstractNeurodegenerative diseases, such as Parkinson’s disease, represent a biggest challenge for medicine, imposing high social and economic impacts. As a result, it is of utmost importance to develop new therapeutic strategies. The present work evaluated the neuroprotective potential of seaweeds extracts on an in vitro dopamine (DA)-induced neurotoxicity cellular model. The neuroprotective effects on SH-SY5Y cells’ viability were estimated by the MTT assay. Changes in mitochondrial membrane potential (MMP), caspase-3 activity, and hydrogen peroxide (H2O2) production were determined. DA (30–3000 μM; 24 h) treatment decreased SH-SY5Y cells’ viability in concentration and time-dependent manner, increasing the H2O2 production, MMP depolarization, and caspase-3 activity. On the other hand, DA (1000 μM; 24 h) toxicity was reduced (10–15%) with Sargassum muticum and Codium tomentosum extracts (1000 μg/mL; 24 h). The highest neuroprotective activity was exhibited by a methanolic extract obtained from Saccorhiza polyschides, which completely blunted DA effects. Results show that the marine seaweed S. polyschides contain substances with high neuroprotective potential against the toxicity induced by DA, exhibiting anti-apoptotic effects associated with both mitochondrial protection and caspase-3 inhibition. S. polyschides reveals, therefore, to be an excellent source of bioactive molecules, for new drugs development aiming PD therapeutics.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, J. Alves, C., Pinteus, S., Mendes, S., & Pedrosa, R. (2020). Seaweeds’ neuroprotective potential set in vitro on a human cellular stress model. Molecular and Cellular Biochemistry. DOI:10.1007/s11010-020-03824-5.pt_PT
dc.identifier.doi10.1007/s11010-020-03824-5pt_PT
dc.identifier.issn0300-8177
dc.identifier.urihttp://hdl.handle.net/10400.8/5117
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationUID/MAR/04292/2020pt_PT
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.relationPOCI-01-0145-FEDER-016791pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidantpt_PT
dc.subjectOxidative stresspt_PT
dc.subjectMitochondrial potentialpt_PT
dc.subjectApoptosispt_PT
dc.subjectParkinson’s diseasept_PT
dc.subjectMarine natural compoundspt_PT
dc.subjectAlgaept_PT
dc.titleSeaweeds’ neuroprotective potential set in vitro on a human cellular stress modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-OUT%2F29250%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F103255%2F2014/PT
oaire.citation.titleMolecular and Cellular Biochemistrypt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamPOR_CENTRO
person.familyNameSilva
person.familyNameAlves
person.familyNamePinteus
person.familyNameMENDES
person.familyNamePedrosa
person.givenNameJoana
person.givenNameCelso
person.givenNameSusete
person.givenNameSUSANA
person.givenNameRui
person.identifier98885
person.identifier159091
person.identifier159292
person.identifier322266
person.identifier349272
person.identifier.ciencia-id6317-6F15-BA35
person.identifier.ciencia-id501C-F268-2E60
person.identifier.ciencia-id661E-9677-74C5
person.identifier.ciencia-id4514-12E2-1FD9
person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0001-9681-3169
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id33568138000
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication23e68f76-0f7a-4f39-9da8-f1a26e2e2cd7
relation.isAuthorOfPublicationba5ab7c2-7474-4ea9-9bf0-ca15fe42809a
relation.isAuthorOfPublicationdb45e9a7-9e25-4fbf-903e-9feac4a23d61
relation.isAuthorOfPublicationdb5be03b-2077-482b-a9cf-8b3e84861276
relation.isAuthorOfPublication94496a40-57cf-472f-94e1-5db9d5da78b6
relation.isAuthorOfPublication.latestForDiscoverydb45e9a7-9e25-4fbf-903e-9feac4a23d61
relation.isProjectOfPublication26a2e10e-ee41-49ad-9279-4aeaa596b99f
relation.isProjectOfPublication4e599bd2-3733-4520-8d96-9035a23f9e78
relation.isProjectOfPublication.latestForDiscovery26a2e10e-ee41-49ad-9279-4aeaa596b99f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Silva et al._2020_Seaweeds’ neuroprotective potential set in vitro on a human cellular.pdf
Size:
1.06 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

MARE - Artigos com peer review
ESTM - Artigos em revistas internacionais