Fucus spiralis tissue culture for sustainable phlorotannins production

Pinteus, Susete; Alves, Celso; Horta, André; Silva, Joana; Pedrosa, Rui

http://hdl.handle.net/10400.8/7568

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Abstract(s)

Brown algae have the unique ability to produce phlorotannins, which are phenolics with potent antioxidant capacity. These valuable metabolites have numerous applications in medicine, cosmetics, and food industries. Therefore, it is extremely important to develop strategies to continuously produce these compounds without having to constantly harvest seaweeds from the environment. In this work, the possibility of growing Fucus spiralis under artificial conditions by tissue culture techniques was evaluated and optimized. After growing for four months, callus were excited from the original explants and evaluated for phlorotannin content by the Folin- Ciocalteu method and scavenging potential by the 2,2-diphenyl-2-picrilhidrazil (DPPH) scavenging assay. Phlorotannin content and scavenging potential were compared with wild-collected tissue. Fucus spiralis grown callus exposed to 60 μmol photons.m-2.s-1 presented the highest phlorotannins’ content and the highest scavenging potential with 250.02 ± 7.24 mg phloroglucinol equivalents/ g extract and an IC50 of 125.2 (101.3 - 139.5) μg/mL, respectively. This research represents the first evaluation of the potential of Fucus spiralis to be cultured under artificial conditions for phlorotannins extraction, opening a new window for further optimization and development of photobioreactors for continuous phlorotannins production.

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Acknowledgments : This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the strategic project UIDP/04292/2020 and UIDB/04292/2020 granted to MARE—Marine and Environmental Sciences Center, and through CROSS-ATLANTIC project (PTDC/BIAOUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER029250).

Keywords

Antioxidant activity Brown algae Fucales Marine natural compounds Photobioreactor Seaweeds aquaculture

URI

http://hdl.handle.net/10400.8/7568

Citation

Susete Pinteus, Celso Alves, André Horta, Joana Silva, Rui Pedrosa, Fucus spiralis tissue culture for sustainable phlorotannins production, Aquatic Botany, Volume 179, 2022, 103512, ISSN 0304-3770, https://doi.org/10.1016/j.aquabot.2022.103512