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Neuromodulatory effects of Calyptranthes grandifolia extracts against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells

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dc.contributor.authorKich, Débora Mara
dc.contributor.authorBitencourt, Shanna
dc.contributor.authorAlves, Celso
dc.contributor.authorSilva, Joana
dc.contributor.authorPinteus, Susete
dc.contributor.authorPedrosa, Rui
dc.contributor.authorLaufer, Stefan
dc.contributor.authorSouza, Claucia Fernanda Volken de
dc.contributor.authorGoettert, Márcia Inês
dc.date.accessioned2021-08-24T15:28:02Z
dc.date.available2021-08-24T15:28:02Z
dc.date.issued2016
dc.description.abstractAlzheimer’s and Parkinson's diseases are neurodegenerative disorders characterized by progressive neuronal dysfunction. Previous studies revealed that some natural products have neuroprotective properties, including species of the Myrtaceae family. However, the neuromodulatory potential of Calyptranthes grandifolia is not clear. In the present study, we examined the ability of the ethanol and hexane leaf extracts of C. grandifolia to prevent 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in vitro. Initially, we investigated the potential of the extracts to inhibit the neurodegenerative-related enzymes c-Jun N-terminal kinase 3 (JNK3) and acetylcholinesterase (AChE). In addition, SH-SY5Y cell viability was assessed by MTT assay after 100 mM 6-OHDA-induced cell damage. In order to verify the possible effects of both extracts on 6-OHDA-induced cell death, hydrogen peroxide generation, mitochondrial potential and caspases-3 activity were assessed. Our findings revealed that ethanol extract exhibited inhibitory activity against JNK3 and AChE. In addition, when co-treating SH-SY5Y cells with 6-OHDA and the extracts, oxidative stress was inhibited by both extracts through a decrease of mitochondrial depolarization and caspases-3 activity. In summary, ethanol and hexane extracts of C. grandifolia have some suppressive property against neurotoxicity induced by 6-OHDA.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDébora Mara Kich, Shanna Bitencourt, Celso Alves, Joana Silva, Susete Pinteus, Rui Pedrosa, Stefan Laufer, Claucia Fernanda Volken de Souza, Márcia Inês Goettert, Neuromodulatory effects of Calyptranthes grandifolia extracts against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells, Biomedicine & Pharmacotherapy, Volume 84, 2016, Pages 382-386, ISSN 0753-3322, https://doi.org/10.1016/j.biopha.2016.09.063.pt_PT
dc.identifier.doi10.1016/j.biopha.2016.09.063pt_PT
dc.identifier.issn0753-3322
dc.identifier.urihttp://hdl.handle.net/10400.8/6148
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0753332216309477pt_PT
dc.subjectPhytopharmacologypt_PT
dc.subjectNeurodegenerative diseasespt_PT
dc.subject6-OHDApt_PT
dc.subjectJNK3pt_PT
dc.subjectMitochondrial potentialpt_PT
dc.subjectCaspase-3pt_PT
dc.titleNeuromodulatory effects of Calyptranthes grandifolia extracts against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage386pt_PT
oaire.citation.startPage382pt_PT
oaire.citation.titleBiomedicine & Pharmacotherapypt_PT
oaire.citation.volume84pt_PT
person.familyNameAlves
person.familyNameSilva
person.familyNamePinteus
person.familyNamePedrosa
person.givenNameCelso
person.givenNameJoana
person.givenNameSusete
person.givenNameRui
person.identifier159091
person.identifier98885
person.identifier159292
person.identifier349272
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person.identifier.ciencia-id661E-9677-74C5
person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id7005010300
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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