Loading...
Publication
Seasonal evaluation of Pholorotannin-Enriched extracts from brown macroalgae Fucus spiralis
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.43 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Fucus spiralis that was collected in the four seasons was submitted to an extraction with ethanol:water (crude extracts Et80), followed by a liquid–liquid fractionation with organic solvents (fraction He from n-hexane; aqueous fractions AQ1, AQ2, AQ3 and AQ4; ethyl acetate fraction EA), with the aim of obtaining phlorotannin-enriched extracts. All the extracts (Et80, He, AQ1, AQ2, AQ3, AQ4 and EA) that were obtained for the F. spiralis of the four seasons were evaluated for their antioxidant capacity and total phenolic compounds. The summer extracts presented the highest contents in polyphenols (TPC), as well as the highest ferric reducing antioxidant power (FRAP), when compared to the samples from the other seasons. The reductive percentage of the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) compound was similar between the seasons. For all the seasons, the EA extract showed the highest polyphenol content (TPC), and the highest antioxidant capacity (highest ferric reducing power (FRAP) and lowest concentration needed to reduce 50% of the DPPH compound), which is in agreement with a phlorotannin-enriched fraction. This study revealed that the polyphenol content and antioxidant power of the F. spiralis extracts are influenced by the time of harvest, as well as by the solvents used for their extraction.
Description
Funding: This research was funded by FCT (Fundação para a Ciência e a Tecnologia), grant number UID/MAR/04292/2020. This work was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018). The work performed at FCT NOVA was supported by the Applied Molecular Biosciences Unit—UCIBIO, which is financed by national funds from FCT (UIDP/04378/2020 and UIDB/04378/2020). The NMR spectrometers at FCT NOVA are part of Rede Nacional de RMN (PTNMR), supported by FCT—Fundação para a Ciência e Tecnologia (ROTEIRO/0031/2013 – PINFRA/22161/2016) co-financed by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC).
Keywords
Fucus spiralis Antioxidant activity Polyphenols Phlorotannins
Citation
Almeida B, Barroso S, Ferreira ASD, Adão P, Mendes S, Gil MM. Seasonal Evaluation of Phlorotannin-Enriched Extracts from Brown Macroalgae Fucus spiralis. Molecules. 2021 Jul 15;26(14):4287. doi: 10.3390/molecules26144287. PMID: 34299561; PMCID: PMC8304218.
Publisher
MDPI