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Can Phlorotannins Purified Extracts Constitute a Novel Pharmacological Alternative for Microbial Infections with Associated Inflammatory Conditions?

2012Journal article Open access
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dc.contributor.authorLopes, Graciliana
dc.contributor.authorSousa, Carla
dc.contributor.authorSilva, Luís R.
dc.contributor.authorPinto, Eugénia
dc.contributor.authorAndrade, Paula B.
dc.contributor.authorBernardo, João
dc.contributor.authorMouga, Teresa
dc.contributor.authorValentão, Patrícia
dc.date.accessioned2019-09-06T09:47:22Z
dc.date.available2019-09-06T09:47:22Z
dc.date.issued2012
dc.date.updated2019-09-04T14:53:07Z
dc.description.abstractBacterial and fungal infections and the emerging multidrug resistance are driving interest in fighting these microorganisms with natural products, which have generally been considered complementary to pharmacological therapies. Phlorotannins are polyphenols restricted to brown seaweeds, recognized for their biological capacity. This study represents the first research on the antibacterial, antifungal, anti-inflammatory and antioxidant activity of phlorotannins purified extracts, which were obtained from ten dominant brown seaweeds of the occidental Portuguese coast. Phlorotannins content was determined by the specific dimethoxybenzaldehyde (DMBA) method and a yield between 75 and 969 mg/Kg phloroglucinol units (dry matter) was obtained. Fucus spiralis ranked first, followed by three Cystoseira species. The antiinflammatory potential of the purified extracts was assessed via inhibitory effect on nitric oxide (NO) production by lipopolysaccharide-stimulated RAW 264.7 macrophage cells, Cystoseira tamariscifolia being the one showing promising activity for the treatment of inflammation. NO scavenging ability was also addressed in cell free systems, F. spiralis being the species with highest capacity. The antimicrobial potential of the extracts was checked against five Gram-positive and four Gram-negative bacteria and three fungi strains, that commonly colonize skin and mucosa and are responsible for food contamination. The different extracts were more effective against Gram-positive bacteria, Staphylococcus epidermidis being the most susceptible species. Concerning antifungal activity, Trichophyton rubrum was the most sensitive species. Although the molecular mechanisms underlying these properties remain poorly understood, the results obtained turn phlorotannins purified extracts a novel and potent pharmacological alternative for the treatment of a wide range of microbial infections, which usually also present an inflammatory component. In addition to the biological properties demonstrated herein, phlorotannins extracts may also be preferred, in order to avoid side effects and allergic reactions commonly associated with synthetic drugs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLopes G, Sousa C, Silva LR, Pinto E, Andrade PB, et al. (2012) Can Phlorotannins Purified Extracts Constitute a Novel Pharmacological Alternative for Microbial Infections with Associated Inflammatory Conditions? PLoS ONE 7(2): e31145. doi:10.1371/journal.pone.0031145pt_PT
dc.identifier.doi10.1371/journal.pone.0031145
dc.identifier.otherWOS:000301979000022
dc.identifier.slugcv-prod-149962
dc.identifier.urihttp://hdl.handle.net/10400.8/4065
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationStrategic Project - LA 6 - 2011-2012
dc.relationMACROALGAE FROM BERLENGAS ARCHIPELAGO AS SOURCE OF BIOACTIVE COMPOUNDS
dc.relation.ispartofPLoS ONEen_US
dc.titleCan Phlorotannins Purified Extracts Constitute a Novel Pharmacological Alternative for Microbial Infections with Associated Inflammatory Conditions?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardTitleMACROALGAE FROM BERLENGAS ARCHIPELAGO AS SOURCE OF BIOACTIVE COMPOUNDS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F61565%2F2009/PT
oaire.citation.endPagee31145pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPagee31145pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume7pt_PT
oaire.fundingStream6820 - DCRRNI ID
person.familyNameMouga
person.givenNameTeresa
person.identifier.ciencia-idE312-FDCC-1ED8
person.identifier.orcid0000-0003-4849-8840
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidE312-FDCC-1ED8 | Teresa Mouga
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication901e8c7b-9818-4493-8e96-75d0c5ad718c
relation.isAuthorOfPublication.latestForDiscovery901e8c7b-9818-4493-8e96-75d0c5ad718c
relation.isProjectOfPublication4ef7a632-13d0-492f-ac17-5b6f22236ced
relation.isProjectOfPublicationd41ae0b4-6231-4e6d-b6c0-18923a048c4e
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