Repository logo
 
Publication

Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents

2020Journal article Restricted access
Simple item page
dc.contributor.authorAlves, Celso
dc.contributor.authorSerrano, Eurico
dc.contributor.authorSilva, Joana
dc.contributor.authorRodrigues, Carlos
dc.contributor.authorPinteus, Susete
dc.contributor.authorGaspar, Helena
dc.contributor.authorBotana, Luis M.
dc.contributor.authorAlpoim, Maria C.
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2020-08-28T11:11:09Z
dc.date.available2020-08-28T11:11:09Z
dc.date.issued2020
dc.descriptionAcknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-010145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014).
dc.description.abstractCancer is one of the major threats to human health and, due to distinct factors, it is expected that its incidence will increase in the next decades leading to an urgent need of new anticancer drugs development. Ongoing experimental and clinical observations propose that cancer cells with stem-like properties (CSCs) are involved on the development of lung cancer chemoresistance. As tumour growth and metastasis can be controlled by tumourassociated stromal cells, the main goal of this study was to access the antitumor potential of five bromoterpenes isolated from Sphaerococcus coronopifolius red alga to target CSCs originated in a co-culture system of fibroblast and lung malignant cells. Cytotoxicity of compounds (10–500 μM; 72 h) was evaluated on monocultures of several malignant and non-malignant cells lines (HBF, BEAS-2B, RenG2, SC-DRenG2) and the effects estimated by MTT assay. Co-cultures of non-malignant human bronchial fibroblasts (HBF) and malignant human bronchial epithelial cells (RenG2) were implemented and the compounds ability to selectively kill CSCs was evaluated by sphere forming assay. The interleucine-6 (IL-6) levels were also determined as cytokine is crucial for CSCs. Regarding the monocultures results bromosphaerol selectively eliminated the malignant cells. Both 12S-hydroxy-bromosphaerol and 12R-hydroxy-bromosphaerol steroisomers were cytotoxic towards non-malignant bronchial BEAS-2B cell line, IC50 of 4.29 and 4.30 μM respectively. However, none of the steroisomers induced damage in the HBFs. As to the co-cultures, 12R-hydroxy-bromosphaerol revealed the highest cytotoxicity and ability to abrogate the malignant stem cells; however its effects were IL-6 independent. The results presented here are the first evidence of the potential of these bromoterpenes to abrogate CSCs opening new research opportunities. The 12R-hydroxy-bromosphaerol revealed to be the most promising compound to be test in more complex living models.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlves, C., Serrano, E., Silva, J., Rodrigues, C., Pinteus, S., Gaspar, H., Botana, L., Alpoim, M., & Pedrosa, Rui. (2020). Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents. Biomedicine & Pharmacotherapy. 128. DOI: 10.1016/j.biopha.2020.110275.pt_PT
dc.identifier.doi10.1016/j.biopha.2020.110275pt_PT
dc.identifier.issn0753-3322
dc.identifier.urihttp://hdl.handle.net/10400.8/5112
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI- 01-0145-FEDER-029250
dc.relationPOCI-01-0145- FEDER-016791
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMarine natural productspt_PT
dc.subjectRed seaweedpt_PT
dc.subjectLung cancerpt_PT
dc.subjectCancer stem cellspt_PT
dc.subjectMicroenvironmentpt_PT
dc.subjectInterleukin-6pt_PT
dc.titleSphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-OUT%2F29250%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F103255%2F2014/PT
oaire.citation.titleBiomedicine and Pharmacotherapypt_PT
oaire.citation.volume128pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamPOR_CENTRO
person.familyNameAlves
person.familyNameSilva
person.familyNamePinteus
person.familyNameGuerreiro Galla Gaspar
person.familyNamePedrosa
person.givenNameCelso
person.givenNameJoana
person.givenNameSusete
person.givenNameHelena Margarida
person.givenNameRui
person.identifier159091
person.identifier98885
person.identifier159292
person.identifierE-6798-2012
person.identifier349272
person.identifier.ciencia-id501C-F268-2E60
person.identifier.ciencia-id6317-6F15-BA35
person.identifier.ciencia-id661E-9677-74C5
person.identifier.ciencia-id761C-044C-995B
person.identifier.ciencia-id3817-33DE-919E
person.identifier.orcid0000-0003-1581-2127
person.identifier.orcid0000-0003-1224-1699
person.identifier.orcid0000-0002-3290-3111
person.identifier.orcid0000-0002-1613-7023
person.identifier.orcid0000-0003-0970-0575
person.identifier.ridB-3366-2017
person.identifier.ridB-4815-2015
person.identifier.scopus-author-id55654969100
person.identifier.scopus-author-id56370819800
person.identifier.scopus-author-id55655328300
person.identifier.scopus-author-id7003891380
person.identifier.scopus-author-id7005010300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationba5ab7c2-7474-4ea9-9bf0-ca15fe42809a
relation.isAuthorOfPublication23e68f76-0f7a-4f39-9da8-f1a26e2e2cd7
relation.isAuthorOfPublicationdb45e9a7-9e25-4fbf-903e-9feac4a23d61
relation.isAuthorOfPublication580efc75-e17e-4380-ae50-e5bc1d0bc10d
relation.isAuthorOfPublication94496a40-57cf-472f-94e1-5db9d5da78b6
relation.isAuthorOfPublication.latestForDiscovery94496a40-57cf-472f-94e1-5db9d5da78b6
relation.isProjectOfPublication26a2e10e-ee41-49ad-9279-4aeaa596b99f
relation.isProjectOfPublication4e599bd2-3733-4520-8d96-9035a23f9e78
relation.isProjectOfPublication.latestForDiscovery26a2e10e-ee41-49ad-9279-4aeaa596b99f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Alves et al._2020_Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents.pdf
Size:
2.52 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

MARE - Artigos com peer review
ESTM - Artigos em revistas internacionais