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Antiulcerogenic potential of the ethanolic extract of Ceiba speciosa (A. St.-Hil.) Ravenna evaluated by in vitro and in vivo studies

2022Journal article Open access
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dc.contributor.authorDörr, Juliana Andréa
dc.contributor.authorMajolo, Fernanda
dc.contributor.authorBortoluzzi, Luísa
dc.contributor.authorVargas, Evelin Zen de
dc.contributor.authorSilva, Joana
dc.contributor.authorPasini, Manoela
dc.contributor.authorStoll, Stefani Natali
dc.contributor.authorRosa, Rafael Lopes da
dc.contributor.authorFigueira, Mariana Moreira
dc.contributor.authorFronza, Marcio
dc.contributor.authorBeys-da-Silva, Walter O.
dc.contributor.authorMartins, Alice
dc.contributor.authorGaspar, Helena
dc.contributor.authorPedrosa, Rui
dc.contributor.authorLaufer, Stefan
dc.date.accessioned2023-08-23T10:33:04Z
dc.date.available2023-08-23T10:33:04Z
dc.date.issued2022
dc.descriptionThis work was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, and Conselho Nacional de Pesquisa (CNPq) 420381/2018-0. This work was also funded by the Portuguese Foundation for Science and Technology (FCT) through the strategic projects granted to the MARE—Marine and Environmental Sciences Center (UIDP/04292/2020; UIDB/04292/2020), the BioISI—BioSystems and Integrative Sciences Institute (UIDP/Multi/04046/2020; UIDB/04046/2020) and the Associate Laboratory ARNET (LA/P/0069/2020). FCT and CAPES cooperation agreement also support this study through project MArTics (FCT/DRI/CAPES 2019.00277.CBM). The Germany part was funded by Eberhard Karls Universität Tübingen.pt_PT
dc.description.abstractGastrointestinal diseases, such as peptic ulcers, are caused by a damage in the gastric mucosa provoked by several factors. This stomach injury is regulated by many inflammatory mediators and is commonly treated with proton-pump inhibitors, histamine H2 receptor blockers and antacids. However, various medicinal plants have demonstrated positive effects on gastric ulcer treatment, including plants of the Ceiba genus. The aim of this study was to evaluate the antiulcer and anti-inflammatory activities of the stem bark ethanolic extract of Ceiba speciosa (A. St.-Hil.) Ravenna. We performed a preliminary quantification of phenolic compounds by high-performance liquid chromatography-diode array detection (HPLC-DAD), followed by the prospection of other chemical groups through nuclear magnetic resonance (NMR) spectroscopy. A set of in vitro assays was used to evaluate the extract potential regarding its antioxidant activity (DPPH: 19.83 +- 0.34 ug/mL; TPC: 307.20 +- 6.20 mg GAE/g of extract), effects on cell viability and on the release of TNF-α in whole human blood. Additionally, in vivo assays were performed to evaluate the leukocyte accumulation and total protein quantification in carrageenan-induced air pouch, as well as the antiulcerogenic effect of the extract on an ethanol-induced ulcer in rats. The extract contains flavonoids and phenolic compounds, as well as sugars and quinic acid derivatives exhibiting potent antioxidant activity and low toxicity. The extract reduced the release of TNF-α in human blood and inhibited the activity of p38α (1.66 ug/mL), JAK3 (5.25 ug/mL), and JNK3 (8.34 ug/mL). Moreover, it reduced the leukocyte recruitment on the pouch exudate and the formation of edema, reverting the effects caused by carrageenan. The extract presented a significant prevention of ulcer formation and a higher reduction than the reference drug, Omeprazole. Therefore, C. speciosa extract has demonstrated relevant therapeutic potential for the treatment of gastric diseases, deserving the continuation of further studies to unveil the mechanisms of action of plant bioactive ingredients.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDörr, J.A.; Majolo, F.; Bortoluzzi, L.; de Vargas, E.Z.; Silva, J.; Pasini, M.; Stoll, S.N.; da Rosa, R.L.; Figueira, M.M.; Fronza, M.; et al. Antiulcerogenic Potential of the Ethanolic Extract of Ceiba speciosa (A. St.-Hil.) Ravenna Evaluated by In Vitro and In Vivo Studies. Int. J. Mol. Sci. 2022, 23, 15634. https://doi.org/ 10.3390/ijms232415634pt_PT
dc.identifier.doi10.3390/ijms232415634pt_PT
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.8/8725
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.relationBiosystems and Integrative Sciences Institute
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/24/15634pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectAnti-inflammatorypt_PT
dc.subjectCeiba genuspt_PT
dc.subjectGastrointestinal diseasespt_PT
dc.subjectInflammationpt_PT
dc.subjectPeptic ulcerpt_PT
dc.titleAntiulcerogenic potential of the ethanolic extract of Ceiba speciosa (A. St.-Hil.) Ravenna evaluated by in vitro and in vivo studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
oaire.citation.issue24pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
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project.funder.nameFundação para a Ciência e a Tecnologia
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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