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Publicação
A multimodal stimulation cell culture bioreactor for tissue engineering: A numerical modelling approach
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Orientador(es)
Resumo(s)
The use of digital twins in tissue engineering (TE) applications is of paramount importance to reduce the number of in vitro and in vivo tests. To pursue this aim, a novel multimodal bioreactor is developed, combining 3D design with numerical stimulation. This approach will facilitate the reproducibility between studies and the platforms optimisation (physical and digital) to enhance TE. The new bioreactor was specifically designed to be additive manufactured, which could not be reproduced with conventional techniques. Specifically, the design suggested allows the application of dual stimulation (electrical and mechanical) of a scaffold cell culture. For the selection of the most appropriate material for bioreactor manufacturing several materials were assessed for their cytotoxicity. Numerical modelling methods were then applied to the new bioreactor using one of the
most appropriate material (Polyethylene Terephthalate Glycol-modified (PETG)) to find the optimal
stimulation input parameters for bone TE based on two reported in vitro studies.
Descrição
This research was funded by the Fundação para a Ciência e a Tecnologia (FCT) and Centro2020 through
the following Projects: UIDP/04044/2020, PAMI—ROTEIRO/0328/2013 (No 022158) and Stimuli2BioScaffold—
Stimuli modelling for BioScaffolds: from numerical modelling to in vitro tests co-financed by COMPETE2020
under the PT2020 programme, and supported by FCT (02/SAICT/2017). Ref. POCI-01-0145-FEDER-032554;
Bone2Move—Development of “in vivo” experimental techniques and modeling methodologies for the evaluation
of 4D scaffolds on bone defect in sheep model: an integrative research approach co-financed by COMPETE2020 under the PT2020 programme, and supported by FCT (02/SAICT/2017), Project nº 31146; MATIS—MATERIALS AND SUSTAINABLE INDUSTRIAL TECHNOLOGIES (CENTRO-01-0145-FEDER-000014-3362); Instituto de Biofísica e Engenharia Biomédica (IBEB) is supported by Fundação para a Ciência e Tecnologia (FCT), Portugal, under Grant n UIDB/00645/2020. Also supported by UID/BIO/04565/2020.
Palavras-chave
Cylindrical perfusion bioreactor Multimodal stimulation Cytotoxicity study Material characterization Bone tissue engineering Finite element analysis Electrical stimulation
Contexto Educativo
Citação
Meneses, J.; C. Silva, J.; R. Fernandes, S.; Datta, A.; Castelo Ferreira, F.; Moura, C.; Amado, S.; Alves, N.; Pascoal-Faria, P. A Multimodal Stimulation Cell Culture Bioreactor for Tissue Engineering: A Numerical Modelling Approach. Polymers 2020, 12, 940. https://doi.org/10.3390/polym12040940
Editora
MDPI