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Neuroprotective effect of Luteolin-7-O-Glucoside against 6-OHDA-induced damage in undifferentiated and RA-differentiated SH-SY5Y cells

2022Journal article Open access
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dc.contributor.authorRehfeldt, Stephanie Cristine Hepp
dc.contributor.authorSilva, Joana
dc.contributor.authorPinteus, Susete
dc.contributor.authorPedrosa, Rui
dc.contributor.authorAlves, Celso
dc.contributor.authorLaufer, Stefan
dc.contributor.authorGoettert, Márcia Inês
dc.date.accessioned2022-08-22T12:52:46Z
dc.date.available2022-08-22T12:52:46Z
dc.date.issued2022
dc.descriptionFunding: This study was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, and Conselho Nacional de Pesquisa (CNPq) 420381/2018-0. This work was also supported by the Portuguese Foundation for Science and Technology(FCT) through the strategic project UID/04292/2020 granted to MARE—Marine and Environmental Sciences Centre, and UIDP/04046/2020 and UIDB/04046/2020 granted to BioISI—BioSystems and Integrative Sciences Institute, through POINT4PAC project (Oncologia de Precisão: Terapias e Tecnologias Inovadoras, SAICTPAC/0019/2015-LISBOA- 01-0145-FEDER-016405), through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER029250) and through Molecules for Health project (PTDC/BIA-BQM/28355/2017). This work was also supported by FCT and CAPES cooperation agreement through project MArTics (FCT/DRI/CAPES 2019.00277.CBM). We acknowledge support by Open Access Publishing Fund of University of Tübingen.pt_PT
dc.description.abstractLuteolin is one of the most common flavonoids present in edible plants and its potential benefits to the central nervous system include decrease of microglia activation, neuronal damage and high antioxidant properties. The aim of this research was to evaluate the neuroprotective, antioxidant and anti-inflammatory activities of luteolin-7-O-glucoside (Lut7). Undifferentiated and retinoic acid (RA)-differentiated SH-SY5Y cells were pretreated with Lut7 and incubated with 6-hydroxydopamine (6-OHDA). Cytotoxic and neuroprotective effects were determined by MTT assay. Antioxidant capacity was determined by DPPH, FRAP, and ORAC assays. ROS production, mitochondrial membrane potential (DYm), Caspase–3 activity, acetylcholinesterase inhibition (AChEI) and nuclear damage were also determined in SH-SY5Y cells. TNF- , IL-6 and IL-10 release were evaluated in LPS-induced RAW264.7 cells by ELISA. In undifferentiated SH-SY5Y cells, Lut7 increased cell viability after 24 h, while in RA-differentiated SH-SY5Y cells, Lut7 increased cell viability after 24 and 48 h. Lut7 showed a high antioxidant activity when compared with synthetic antioxidants. In undifferentiated cells, Lut7 prevented mitochondrial membrane depolarization induced by 6-OHDA treatment, decreased Caspase-3 and AChE activity, and inhibited nuclear condensation and fragmentation. In LPS-stimulated RAW264.7 cells, Lut7 treatment reduced TNF- levels and increased IL-10 levels after 3 and 24 h, respectively. In summary, the results suggest that Lut7 has neuroprotective effects, thus, further studies should be considered to validate its pharmacological potential in more complex models, aiming the treatment of neurodegenerative diseases.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRehfeldt, S.C.H.; Silva, J.; Alves, C.; Pinteus, S.; Pedrosa, R.; Laufer, S.; Goettert, M.I. Neuroprotective Effect of Luteolin-7-O-Glucoside against 6-OHDA-Induced Damage in Undifferentiated and RA-Differentiated SH-SY5Y Cells. Int. J. Mol. Sci. 2022, 23, 2914. https:// doi.org/10.3390/ijms23062914pt_PT
dc.identifier.doi10.3390/ijms23062914pt_PT
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.8/7573
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUID/04292/2020pt_PT
dc.relationBiosystems and Integrative Sciences Institute
dc.relationBiosystems and Integrative Sciences Institute
dc.relationFCT/DRI/CAPES 2019.00277.CBMpt_PT
dc.relationPOCI-01-0145-FEDER-029250pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/6/2914pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subject6-hydroxydopaminept_PT
dc.subjectApoptosispt_PT
dc.subjectMitochondrial membrane potentialpt_PT
dc.subjectNeurodegenerative diseasespt_PT
dc.subjectOxidative stresspt_PT
dc.subjectCell culture techniquespt_PT
dc.subjectNeurodegenerative disorderspt_PT
dc.subjectNeuroprotective effectpt_PT
dc.subjectBiological productspt_PT
dc.titleNeuroprotective effect of Luteolin-7-O-Glucoside against 6-OHDA-induced damage in undifferentiated and RA-differentiated SH-SY5Y cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04046%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-OUT%2F29250%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BQM%2F28355%2F2017/PT
oaire.citation.issue6pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
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oaire.fundingStream9471 - RIDTI
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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