Sterol profiles in 18 macroalgae of the Portuguese coast

Lopes, Graciliana; Sousa, Carla; Bernardo, João; Andrade, Paula B.; Valentão, Patrícia; Ferreres, Federico; Mouga, Teresa

http://hdl.handle.net/10400.8/8241

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Abstract(s)

The sterol profiles of dominant macroalgae occur-ring in the western Portuguese coast were evaluated.An analytical procedure, involving alkaline hydrolysisand extraction followed by separation by reversed-phase HPLC–diode array detection (HPLC–DAD),was optimized for the study of their sterols composi-tion. The validated methodology is short in analysistime (as the compounds are determined in <20 min),sensitive, reproducible, and accurate. It was thensuccessfully applied to the determination of campes-terol, cholesterol, desmosterol, ergosterol, fucosterol,stigmasterol, andb-sitosterol in 18 species (threeChlorophyta, five Rhodophyta, and 10 Phaeophyta).The profiles obtained for the several macroalgalspecies were considerably different. C29 sterolswere predominant in Phaeophyta and Chlorophyta(71%–95% of total sterol content), while in Rhodo-phyta cholesterol content is significantly higher(34%–87%). Among the studied species,AsparagopsisarmataHarv. contained the lowest sterol amount(555 mgÆkg)1dry weight), andCystoseira tamariscifo-lia(Huds.) Papenf. the highest one (6,502 mgÆkg)1dry weight). Data obtained may be helpful inidentifying suitable marine sources of sterols, withpotential applications in the food and pharmaceutical industries.

Keywords

Chlorophyta Macroalgae Pha-eophyta Rhodophyta Sterols

URI

http://hdl.handle.net/10400.8/8241

Citation

Lopes, G., Sousa, C., Bernardo, J., Andrade, P.B., Valentão, P., Ferreres, F. and Mouga, T. (2011), STEROL PROFILES IN 18 MACROALGAE OF THE PORTUGUESE COAST. Journal of Phycology, 47: 1210-1218. https://doi.org/10.1111/j.1529-8817.2011.01028.x