Optimization of ultrasound-assisted extraction of bioactive compounds from Pelvetia canaliculata to sunflower oil

In this study, Pelvetia canaliculata L. macroalga, collected from the Atlantic Portuguese coast, was used as a source of bioactive compounds, mostly antioxidants, to incorporate them in sunflower oil with the aim of increasing its biological value and oxidative stability. The lyophilized alga was added to the oil, and ultrasound‐assisted extraction (UAE) was performed. Algae concentration and UAE time varied following a central composite rotatable design (CCRD) to optimize extraction conditions. The following parameters were analyzed in the oils: oxidation products, acidity, color, chlorophyll pigments, carotenoids, flavonoids, total phenolic content, antioxidant activity by DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays, and sensory analysis. Extraction conditions did not affect the acidity and the amount of oxidation products in the oil. Chlorophylls and carotenoid contents increased with algae concentration, while flavonoid extraction did not depend on algae content or UAE time. Total phenolics in the oil were highly related only to FRAP antioxidant activity. Storage experiments of supplemented oil (12.5% algae; 20 min UAE) were carried out under accelerated oxidation conditions at 60 °C/12 days. Antioxidant activity (FRAP) of supplemented oil was 6‐fold higher than the value of non‐supplemented oil. Final samples retained 40% of their initial antioxidant activity. The presence of algae extracts contributed to the increased oxidative stability of sunflower oil.

Description

Funding: This research was funded by the Research Project Ocean2Oils “Integrated approach for seaweeds application as sustainable source of functional compounds for edible oils stabilization and food processing” (FA_05_2017_013), co‐funded by Fundo Azul program through the Direção‐ Geral de Política do Mar, Portugal. Acknowledgments: The authors thank Teresa Mouga (Polytechnic of Leiria, Portugal) for the taxonomic identification of Pelvetia canaliculata L., Rita Simões (ISA, Portugal) for helping in antioxidant activity assays, the research units from Fundação para a Ciência e a Tecnologia, I.P.: LEAF (UIDP/04129/2020; UIDB/04129/2020) and MARE (UID/MAR/04292/2020), and the Erasmus Program for the grant to Marija Trifunovska.

Keywords

Algae Antioxidants Bioactive compounds Oxidative stability Pelvetia canaliculata Response surface methodology Sunflower oil Ultrasound-assisted extration

URI

http://hdl.handle.net/10400.8/6729

Citation

Sousa, G.; Trifunovska, M.; Antunes, M.; Miranda, I.; Moldão, M.; Alves, V.; Vidrih, R.; Lopes, P.A.; Aparicio, L.; Neves, M.; et al. Optimization of Ultrasound‐Assisted Extraction of Bioactive Compounds from Pelvetia canaliculata to Sunflower Oil. Foods 2021, 10, 1732. https://doi.org/10.3390/ foods10081732