Logo do repositório
 
Publicação

Production of Human Milk Fat Substitutes Catalyzed by a Heterologous Rhizopus oryzae Lipase and Commercial Lipases

2014-11Artigo científico Acesso restrito
Ver registo simples
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorSimões, Tiago
dc.contributor.authorValero, Francisco
dc.contributor.authorTecelão, Carla
dc.contributor.authorFerreira-Dias, Suzana
dc.date.accessioned2026-07-20T11:58:26Z
dc.date.available2026-07-20T11:58:26Z
dc.date.issued2014-11
dc.description.abstractThis study aims to produce human milk fat substitutes by an acidolysis reaction between lard and the free fatty acids (FFA) from a fish oil concentrate rich in docosahexaenoic acid, in solvent-free media. The immobilized commercial lipases from (1) Rhizomucor miehei (Lipozyme RM IM), (2) Thermomyces lanuginosa (Lipozyme TL IM) and (3) Candida antarctica (Novozym 435) were tested as biocatalyst. Also, the heterologous Rhizopus oryzae lipase (rROL), immobilized in Accurel MP 1000, was tested as a feasible alternative to the commercial lipases. After 24 h of reaction at 50 C, similar incorporations of polyunsaturated fatty acids (c.a. 17 mol%) were attained with Novozym 435, Lipozyme RM IM and rROL. The lowest incorporation was achieved with Lipozyme TL IM (7.2 mol%). Modeling acidolysis catalyzed by rROL and optimization of reaction conditions were performed by response surface methodology, as a function of the molar ratio FFA/lard and the temperature. The highest acidolysis activity was achieved at 40 C at a molar ratio of 3:1, decreasing with both temperature and molar ratio. Operational stability studies for rROL in seven consecutive 24-h batches were carried out. After the fourth batch, the biocatalyst retained about 55 % of the original activity (half-life of 112 h).eng
dc.description.sponsorshipThis study was supported by the: (1) Program CYTED, under the scope of the ‘‘Ibero-American Network for the Extraction and Enzymatic Transformation of Functional Ingredients and Nutraceuticals from Regional Plants and Agro-Residues (ENZNUT)’’, (2) by the project CTQ2010-15131—of the Spanish Ministry of Science and Innovation, (3) by 2009-SGR-281 and Reference Network in Biotechnology (XRB) (Generalitat de Catalunya), (4) by the Strategic Project PEst-OE/AGR/UI0245/2011 of CEER, Biosystems Engineering, a research unit supported by the national funding of Fundac¸a˜o para a Cieˆncia e a Tecnologia, Portugal, and by (5) the Integrated Action Portugal-Spain E-29/11.
dc.identifier.citationSimões, T., Valero, F., Tecelão, C. and Ferreira-Dias, S. (2014), Production of Human Milk Fat Substitutes Catalyzed by a Heterologous Rhizopus oryzae Lipase and Commercial Lipases. J Am Oil Chem Soc, 91: 411-419. https://doi.org/10.1007/s11746-013-2379-9
dc.identifier.doi10.1007/s11746-013-2379-9
dc.identifier.issn0003-021X
dc.identifier.issn1558-9331
dc.identifier.urihttp://hdl.handle.net/10400.8/16618
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relation.hasversionhttps://aocs.onlinelibrary.wiley.com/journal/15589331
dc.relation.ispartofJournal of the American Oil Chemists' Society
dc.rights.uriN/A
dc.subjectAcidolysis
dc.subjectHuman milk fat substitutes
dc.subjectLard
dc.subjectOperational stability
dc.subjectPolyunsaturated fatty acids
dc.subjectRecombinant lipase
dc.subjectRhizopus oryzae
dc.subjectStructured lipids
dc.titleProduction of Human Milk Fat Substitutes Catalyzed by a Heterologous Rhizopus oryzae Lipase and Commercial Lipaseseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage419
oaire.citation.issue3
oaire.citation.startPage411
oaire.citation.titleJournal of the American Oil Chemists' Society
oaire.citation.volume91
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameSimões
person.familyNameTecelão
person.familyNameFerreira-Dias
person.givenNameTiago
person.givenNameCarla
person.givenNameSuzana
person.identifier.ciencia-id0D1D-8D1A-A883
person.identifier.ciencia-id9B1D-4AC0-1B03
person.identifier.ciencia-idAA14-7B2F-5098
person.identifier.orcid0000-0002-6107-9790
person.identifier.orcid0000-0003-2423-0495
person.identifier.orcid0000-0003-3655-8201
person.identifier.ridE-1128-2013
person.identifier.scopus-author-id16302507900
person.identifier.scopus-author-id7801371939
person.identifier.scopus-author-id6603615381
relation.isAuthorOfPublication5699372e-8c6b-40ff-b5e9-af28324980e3
relation.isAuthorOfPublication0d890808-4e69-4e6c-a631-802f1a7dce1f
relation.isAuthorOfPublication69965b8b-e2fd-4212-b9b5-904eb9b996fa
relation.isAuthorOfPublication.latestForDiscovery5699372e-8c6b-40ff-b5e9-af28324980e3

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Production of Human Milk Fat Substitutes Catalyzed by a Heterologous Rhizopus oryzae Lipase and Commercial Lipases.pdf
Tamanho:
506.32 KB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.32 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

MARE - Artigos com peer review
ESTM - Artigos em revistas internacionais