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Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model

2019Journal article Open access
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dc.contributor.authorSilva, Joana
dc.contributor.authorAlves, Celso
dc.contributor.authorFreitas, Rafaela
dc.contributor.authorMartins, Alice
dc.contributor.authorPinteus, Susete
dc.contributor.authorRibeiro, Joana
dc.contributor.authorGaspar, Helena
dc.contributor.authorAlfonso, Amparo
dc.contributor.authorPedrosa, Rui
dc.date.accessioned2020-08-12T10:51:45Z
dc.date.available2020-08-12T10:51:45Z
dc.date.issued2019
dc.descriptionThis work was supported by the Portuguese Foundation for Science and Technology (FCT) through Strategic Projects UID/MAR/04292/2013 and UID/Multi/04046/2019 granted to MARE—Marine and Environmental Sciences Centre, and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery Project (PTDC/MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 -LISBOA-01-0145-FEDER-016405) and through CrossAtlantic Project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund.pt_PT
dc.descriptionAuthors are very grateful for the financial support of the Projects and Programmes described in funding section. FCT is also acknowledged for the grants attributed to JS (SFRH/BD/103255/2014), CA (SFRH/BD/97764/2013), and SP (SFRH/BD/96203/2013).pt_PT
dc.description.abstractBifurcaria bifurcata is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1–F7) obtained by normal phase chromatography from the B. bifurcata dichloromethane extract, as well as of its two major isolated diterpenes. Total phenolic content of fractions was determined by the Folin–Ciocalteu method, while antioxidant activity was evaluated by the DPPH, ORAC, and FRAP assays. Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H2O2 production, Caspase-3 activity, and by observation of DNA fragmentation. Fractions F4 and F5 exhibited the best neuroprotective and antioxidant activities, respectively. F4 fraction prevented changes in mitochondrial potential, and induced a reduction of H2O2 levels production and an increase in cell viability, suggesting that it may contain multi-target compounds acting on different pathways. Hence, this fraction was subjected to purification steps, affording the known diterpenes eleganolone and eleganonal. Both compounds exhibited antioxidant potential, being interesting candidates for further neuroprotective studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, J., Alves, C., Freitas, R., Martins, A., Pinteus, S., Ribeiro, J., Gaspar, H., Alfonso, A., & Pedrosa, R. (2019). Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson's Disease Model. Marine drugs, 17(2), 85. https://doi.org/10.3390/md17020085pt_PT
dc.identifier.doi10.3390/md17020085pt_PT
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.8/5071
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPOCI-01-0145-FEDER-029250pt_PT
dc.relationPOCI-01-0145-FEDER-016791pt_PT
dc.relationBiosystems & Integrative Sciences Institute
dc.relationNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subject6-hydroxydopaminept_PT
dc.subjectSeaweedspt_PT
dc.subjectSH-SY5Y cellspt_PT
dc.subjectNeuroprotectionpt_PT
dc.subjectMitochondrial membrane potentialpt_PT
dc.subjectCaspase-3 activitypt_PT
dc.subjectEleganolonept_PT
dc.subjectEleganonalpt_PT
dc.subjectNeurodegenerative diseasespt_PT
dc.titleAntioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosystems & Integrative Sciences Institute
oaire.awardTitleNeuroprotective effects of molecules isolated from Bifurcaria bifurcata and Codium tomentosum in an in vitro and in vivo Parkinson Disease model.
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oaire.citation.issue2pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume17pt_PT
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person.familyNameSilva
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