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ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis

2013-10Artigo científico Acesso restrito
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Industrial
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg08:Trabalho Digno e Crescimento Económico
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorRodrigues, Américo
dc.contributor.authorAdamo, Mattia
dc.contributor.authorCrozet, Pierre
dc.contributor.authorMargalha, Leonor
dc.contributor.authorConfraria, Ana
dc.contributor.authorMartinho, Cláudia
dc.contributor.authorElias, Alexandre
dc.contributor.authorRabissi, Agnese
dc.contributor.authorLumbreras, Victoria
dc.contributor.authorGonzález-Guzmán, Miguel
dc.contributor.authorAntoni, Regina
dc.contributor.authorRodriguez, Pedro L.
dc.contributor.authorBaena-González, Elena
dc.date.accessioned2026-03-23T15:28:11Z
dc.date.available2026-03-23T15:28:11Z
dc.date.issued2013-10
dc.description.abstractPlant survival under environmental stress requires the integration of multiple signaling pathways into a coordinated response, but the molecular mechanisms underlying this integration are poorly understood. Stress-derived energy deprivation activates the Snf1-related protein kinases1 (SnRK1s), triggering a vast transcriptional and metabolic reprogramming that restores homeostasis and promotes tolerance to adverse conditions. Here, we show that two clade A type 2C protein phosphatases (PP2Cs), established repressors of the abscisic acid (ABA) hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation. Accordingly, SnRK1 repression is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpression, sugar hypersensitivity during early seedling development. Reporter gene assays and SnRK1 target gene expression analyses further demonstrate that PP2C inhibition by ABA results in SnRK1 activation, promoting SnRK1 signaling during stress and once the energy deficit subsides. Consistent with this, SnRK1 and ABA induce largely overlapping transcriptional responses. Hence, the PP2C hub allows the coordinated activation of ABA and energy signaling, strengthening the stress response through the cooperation of two key and complementary pathways.eng
dc.description.sponsorshipWe thank Vera Nunes for plant management, Filip Rolland and Jörg Kudla for comments, and Júlia Costa for help with the scintillation counter. The 35S:SnRK1.1-2 line was provided by Martine Thomas. E.B.-G. was supported by grants from Marie Curie IRG, the EMBO Installation program, Marie Curie Actions FP7-People-2010-ITN, the Fundação para a Ciência e a Tecnologia (FCT-PTDC/AGR-AM/104939/2008), and the Portugal-Spain Bilateral Collaboration program Ações integradas (Ação E-26/10). A.C. was supported by SFRH/BPD/47280/2008, C.M. was supported by SFRH/BD/33563/2008, L.M. was supported by SFRH/BD/51627/2011, and P.C. was supported by SFRH/BPD/79255/2011. A. Rabissi was supported by a Generalitat de Catalunya PhD grant (FI-AR067443). P.L.R. was supported by the Ministerio de Ciencia e Innovación (grants BIO2011-23446 and PT2009-0155), R.A. was supported by the Junta para Ampliación de Estudios e Investigaciones Científicas-Consejo Superior de Investigaciones Cientificas fellowship, and M.G.-G. was supported by a Juan de la Cierva contract.
dc.identifier.citationAmérico Rodrigues, Mattia Adamo, Pierre Crozet, Leonor Margalha, Ana Confraria, Cláudia Martinho, Alexandre Elias, Agnese Rabissi, Victoria Lumbreras, Miguel González-Guzmán, Regina Antoni, Pedro L. Rodriguez, Elena Baena-González, ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis , The Plant Cell, Volume 25, Issue 10, October 2013, Pages 3871–3884, https://doi.org/10.1105/tpc.113.114066
dc.identifier.doi10.1105/tpc.113.114066
dc.identifier.issn1040-4651
dc.identifier.issn1532-298X
dc.identifier.urihttp://hdl.handle.net/10400.8/15947
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford University Press (OUP)
dc.relation.hasversionhttps://academic.oup.com/plcell/article-abstract/25/10/3871/6099517?redirectedFrom=fulltext
dc.relation.ispartofThe Plant Cell
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsiseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3884
oaire.citation.issue10
oaire.citation.startPage3871
oaire.citation.titleThe Plant Cell
oaire.citation.volume25
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues
person.givenNameAmérico
person.identifier.orcid0000-0002-5293-5583
person.identifier.ridF-9573-2010
person.identifier.scopus-author-id15745701600
relation.isAuthorOfPublication718ad6e8-1d3a-4ce3-9870-e4ed6732c30b
relation.isAuthorOfPublication.latestForDiscovery718ad6e8-1d3a-4ce3-9870-e4ed6732c30b

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