Boosting antimicrobial activity of ciprofloxacin by functionalization of mesoporous silica nanoparticles

Mesoporous silica nanoparticles (MSNs) are very promising nanomaterials for treating bacterial infections when combined with pharmaceutical drugs. Herein, we report the preparation of two nanomaterials based on the immobilization of ciprofloxacin in mesoporous silica nanoparticles, either as the counter-ion of the choline derivative cation (MSN-[Ch][Cip]) or via anchoring on the surface of amino-group modified MSNs via an amide bond (MSN-Cip). Both nanomaterials were characterized by TEM, FTIR and solution 1H NMR spectroscopies, elemental analysis, XRD and N2 adsorption at 77 K in order to provide the desired structures. No cytotoxicity from the prepared mesoporous nanoparticles on 3T3 murine fibroblasts was observed. The antimicrobial activity of the nanomaterials was determined against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Klebsiella pneumoniae) bacteria and the results were promising against S. aureus. In the case of B. subtilis, both nanom aterials exhibited higher antimicrobial activity than the precursor [Ch][Cip], and in the case of K. pneumoniae they exhibited higher activity than neutral ciprofloxacin.

Description

The authors also thank Fundação para a Ciência e Tecnologia for the projects PTDC/QUI-QOR/32406/2017, PEst-C/LA0006/2013, RECI/BBBBQB/0230/2012 as well as MAR2020 (MAR-02.01.01-FEAMP-0042–INOVA4AQUA). The NMR spectrometers are part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project N◦ 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC). This work was supported by the Associate Laboratory for Green Chemistry LAQV and MARE—Marine and Environmental Sciences Centre which are financed by national funds from FCT/MCTES (UIDB/50006/2020 and UID/MAR/04292/2020, respectively)

Keywords

Ciprofloxacin Ionic liquids Mesoporous silica nanoparticles Cytotoxicity Antimicrobial activity

URI

http://hdl.handle.net/10400.8/5992

Citation

de Juan Mora, B.; Filipe, L.; Forte, A.; Santos, M.M.; Alves, C.; Teodoro, F.; Pedrosa, R.; Ribeiro Carrott, M.; Branco, L.C.; Gago, S. Boosting Antimicrobial Activity of Ciprofloxacin by Functionalization of Mesoporous Silica Nanoparticles. Pharmaceutics 2021, 13, 218. https:// doi.org/10.3390/pharmaceutics13020218