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Multifunctional gelatin/chitosan electrospunwound dressing dopped with Undaria pinnatifida phlorotannin-enriched extract for skin regeneration

2021Journal article Open access
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dc.contributor.authorFerreira, Carolina A. M.
dc.contributor.authorJanuário, Adriana P.
dc.contributor.authorFélix, Rafael
dc.contributor.authorAlves, Nuno
dc.contributor.authorLemos, Marco F. L.
dc.contributor.authorDias, Juliana R.
dc.date.accessioned2022-08-11T13:20:18Z
dc.date.available2022-08-11T13:20:18Z
dc.date.issued2021
dc.descriptionFunding: This work is supported by the Fundação para a Ciência e a Tecnologia (FCT) through the following Projects: UIDB/04044/2020, UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (Nº 022158) and MATIS (CENTRO-01-0145-FEDER-000014-3362) and SpinningTNT (POCI-01-02B7-FEDER-069285). The authors also wish to acknowledge the support of the European Union through EASME Blue Labs project AMALIA, Algae-to-MArket Lab IdeAs (EASME/EMFF/2016/1.2.1.4/03/SI2.750419), and project VALORMAR (Mobilizing R&TD Programs, Portugal 2020) cofounded by COMPETE (POCI-01-0247-FEDER-024517), the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro- 01-0145-FEDER-000018), ORCHESTRA (POCI-01-0247-FEDER-070155) co-funded by FEDER–Fundo Europeu de Desenvolvimento Regional da União Europeia, Portugal 2020, through COMPETE 2020–Programa Operacional Competitividade e Internacionalização and through FCT.pt_PT
dc.description.abstractThe similarities of electrospun fibers with the skin extracellular matrix (ECM) make them promising structures for advanced wound dressings. Moreover, infection and resistance in wounds are a major health concern that may be reduced with antibacterial wound dressings. In this work, a multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich extract will enhance the wound healing process. Electrospun meshes were produced by incorporating 1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical, mechanical, and biological properties were evaluated for all conditions. Results demonstrated that all developed samples presented a homogenous fiber deposition with the average diameters closer to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched extract enhances the elastic performance of the samples. Additionally, the extract incorporation made the structure stable over time since its in vitro degradation rates decreased under enzymatic medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the mesh’s antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial infection in the wound bed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, C.A.M.; Januário, A.P.; Félix, R.; Alves, N.; Lemos, M.F.L.; Dias, J.R. Multifunctional Gelatin/Chitosan ElectrospunWound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration. Pharmaceutics 2021, 13, 2152. https://doi.org/10.3390/pharmaceutics13122152pt_PT
dc.identifier.doi10.3390/pharmaceutics13122152pt_PT
dc.identifier.issn1999-4923
dc.identifier.urihttp://hdl.handle.net/10400.8/7533
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPOCI-01-0247-FEDER-070155pt_PT
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationCentre for Rapid and Sustainable Product Development
dc.relationPOCI-01-02B7-FEDER-069285pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1999-4923/13/12/2152pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectElectrospun wound dressingpt_PT
dc.subjectMultifunctional structurespt_PT
dc.subjectWound healingpt_PT
dc.subjectChitosan/ gelatin/ phlorotannin-enriched extractpt_PT
dc.subjectChronic woundspt_PT
dc.titleMultifunctional gelatin/chitosan electrospunwound dressing dopped with Undaria pinnatifida phlorotannin-enriched extract for skin regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardTitleCentre for Rapid and Sustainable Product Development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04044%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04044%2F2020/PT
oaire.citation.endPage26pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePharmaceuticspt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMarzia Ferreira
person.familyNameJanuário
person.familyNameFaria da Costa Félix
person.familyNameAlves
person.familyNameLemos
person.familyNameDias
person.givenNameCarolina Alexandra
person.givenNameAdriana P.
person.givenNameRafael Alexandre
person.givenNameNuno
person.givenNameMarco
person.givenNameJuliana
person.identifierKWTr8tQAAAAJ
person.identifier452149
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person.identifier.ciencia-id3215-5FE5-B516
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person.identifier.ciencia-idA315-5CB0-097C
person.identifier.ciencia-id311E-1559-8F6C
person.identifier.ciencia-id0612-D971-F00D
person.identifier.orcid0000-0002-6838-3744
person.identifier.orcid0000-0002-3228-922X
person.identifier.orcid0000-0003-4766-3736
person.identifier.orcid0000-0002-5016-0868
person.identifier.orcid0000-0001-9887-1864
person.identifier.orcid0000-0002-7079-1069
person.identifier.ridN-4073-2013
person.identifier.ridF-7951-2011
person.identifier.ridA-6086-2014
person.identifier.scopus-author-id57200377641
person.identifier.scopus-author-id7006403383
person.identifier.scopus-author-id7006042884
person.identifier.scopus-author-id55749822200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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